TRPC1 Antibody

Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).

Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human prostate. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).

Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human cortex. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRPC1 Antibody Summary

• Immunogen: Peptide with sequence C-NLKQKRDENYQK, from the internal region of the protein sequence according to NP_003295.1; NP_001238774.1.
• Epitope: C-NLKQKRDENYQK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRPC1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection Limit 1:32000

Reactivity Notes

Preditced to cross react with Mouse, Canine and Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Antigen Affinity-purified

Alternate Names for TRPC1 Antibody

• HTRP-1
• MGC133334
• MGC133335
• transient receptor potential canonical 1
• transient receptor potential cation channel, subfamily C, member 1
• transient receptor potential channel 1
• Transient receptor protein 1
• TRP1
• TRP-1
• TRP1short transient receptor potential channel 1
• TRPC1

Background

The TRP1 (TRPC1) gene encodes for a short transient receptor potential channel 1 protein which creates non-selective channels permeable to calcium and other cations. The long isoform is 793 amino acids long at around 91 kDA while the short isoform is 759 amino acids at approximately 87 kDA. These proteins are believed to be operated by a phosphatidylinositol messenger system. The TRP1 gene interacts with MX1, CAV1, ITPR3, HOMER1, and BMPR2 genes in pathways for CREB transcription, intracellular calcium signaling, developmental biology and pancreatic secretion. The TRP1 gene has been studied in various diseases such as kidney disease, breast cancer, neuroblastoma, prostatius, retinitis, basal cell carcinoma, muscular dystrophy, gingivitis, hypoxia, cerebritis, neurodegeneration, pyloric stenosis, and bipolar disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPC1