Ankyrin 1 Antibody

Images

 
Independent Antibodies: Western Blot: Ankyrin 1 Antibody [NBP1-71805] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Ankyrin 1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1831 and 1881 of human Ankyrin 1 using the numbering given in entry NP_065209.2 (GeneID 286).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE
Reviewed Applications
Read 1 Review rated 4
using
NBP1-71805 in the following applications:

Publications
Read Publications using
NBP1-71805 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ankyrin 1 Antibody

  • ANK
  • ANK-1
  • ankyrin 1, erythrocytic
  • ankyrin-1
  • ankyrin-R
  • Erythrocyte ankyrin
  • SPH1
  • SPH2

Background

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actincytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and themaintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various targetproteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed ofthree structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highlyconserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subjectto variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has alsobeen found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of allpatients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving riseto different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting fromusage of an alternate promoter have also been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ankyrin 1 Antibody (NBP1-71805)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(2)
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Human
(2)
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Review for Ankyrin 1 Antibody (NBP1-71805) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-71805:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Ankyrin 1 NBP1-71805
Enlarge
4
reviewed by:
Wei-Ting Lu
WB Human 05/20/2015
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Summary

ApplicationWestern Blot
Sample Testedhuman a549
SpeciesHuman
Lota1

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Wei-Ting Lu
05/20/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ANK1
Entrez
Uniprot