Ankyrin 1 Antibody

Western Blot: Ankyrin 1 Antibody [NBP1-71805] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-Ankyrin 1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Ankyrin 1 was also immunoprecipitated by another rabbit anti-Ankyrin 1 antibody, which recognizes an upstream epitope.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Ankyrin 1 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 1831 and 1881 of human Ankyrin 1 using the numbering given in entry NP_065209.2 (GeneID 286).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ankyrin 1 Antibody

• ANK
• ANK-1
• ankyrin 1, erythrocytic
• ankyrin-1
• ankyrin-R
• Erythrocyte ankyrin
• SPH1
• SPH2

Background

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actincytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and themaintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various targetproteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed ofthree structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highlyconserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subjectto variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has alsobeen found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of allpatients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving riseto different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting fromusage of an alternate promoter have also been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ankyrin 1 Antibody (NBP1-71805)(2)

Publications using NBP1-71805

• Tessema M, Yingling CM, Picchi MA et al. ANK1 Methylation regulates expression of MicroRNA-486-5p and discriminates lung tumors by histology and smoking status. Cancer Lett. 2017 Sep 29 [PMID: 28965852] (WB, Human)
• Hall AE, Lu WT, Godfrey JD et al. The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration. Cell Death Dis. 2016 Apr 08 [PMID: 27054339] (WB, Human)

Review for Ankyrin 1 Antibody (NBP1-71805) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Wei-Ting Lu WB Human 05/20/2015

Summary

• Application: Western Blot
• Sample Tested: human a549
• Species: Human
• Lot: a1

Bioinformatics

• Gene Symbol: ANK1
• Entrez:
  • 286(Human)
• Uniprot:
  • P16157(Human)