Home » Ankyrin 1 » Ankyrin 1 Antibodies » Ankyrin 1 Antibody

Ankyrin 1 Antibody

 (2 Publications)
 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: Ankyrin 1 Antibody [NBP1-71805] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-Ankyrin 1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Ankyrin 1 was also immunoprecipitated by another rabbit anti-Ankyrin 1 antibody, which recognizes an upstream epitope.
Independent Antibodies: Western Blot: Ankyrin 1 Antibody [NBP1-71805] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

Order Details

100% Guarantee on every product

Ankyrin 1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1831 and 1881 of human Ankyrin 1 using the numbering given in entry NP_065209.2 (GeneID 286).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE
Reviewed Applications
Read 1 Review rated 4
using
NBP1-71805 in the following applications:
Publications
Read Publications using
NBP1-71805 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ankyrin 1 Antibody

  • ANK
  • ANK-1
  • ankyrin 1, erythrocytic
  • ankyrin-1
  • ankyrin-R
  • Erythrocyte ankyrin
  • SPH1
  • SPH2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

ENPP-1 Antibody
NBP2-27561
Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Staining of paraffin embedded Human Thyroid Gland at dilution of 8ug/ml. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: ENPP-1 Antibody [NBP2-27561] - Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.
(7 Publications)
Species: Ch, Hu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
ANKH Antibody
NBP2-92843
Western Blot: ANKH Antibody [NBP2-92843] - Analysis of extracts of various cell lines, using ANKH .Exposure time: 60s.
Immunohistochemistry-Paraffin: ANKH Antibody [NBP2-92843] - Paraffin-embedded human prostate using ANKH .
Species: Hu
Applications: IHC, IHC-P, WB
OBSCN Antibody
NBP1-88818
Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: OBSCN Antibody [NBP1-88818] - Analysis in human skeletal muscle and liver tissues. Corresponding OBSCN RNA-seq data are presented for the same tissues.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Osteopontin/OPN
Antibody [Unconjugate
AF808
Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and secreted molecule. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Osteopontin/OPN (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808). The ND50 is typically 1‑3 µg/mL.
Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(8 Reviews)
(92 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, IHC, Neut, WB
NIK/MAP3K14
Antibody
NBP2-23603
Immunocytochemistry/Immunofluorescence: NIK/MAP3K14 Antibody [NBP2-23603] - MAP3K14 antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: NIK/MAP3K14 Antibody [NBP2-23603] - Analysis of MAP3K14 in mouse small intestine.
Western Blot: NIK/MAP3K14 Antibody [NBP2-23603] - Detection of MAP3K14 in U2OS cell lysate.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Inorganic
Pyrophosphatase/PPA1
NBP1-31348
Immunocytochemistry/Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-31348] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-31348] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:500 dilution.
Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody [NBP1-31348] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 D: Molt-4 12% SDS PAGE; antibody diluted at 1:10000.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Band 3 Antibody
NBP2-49409
Immunohistochemistry: Band 3 Antibody [NBP2-49409] - Staining of human spleen shows strong positivity in erythrocytes.
Immunohistochemistry-Paraffin: Band 3 Antibody [NBP2-49409] - Staining of human bone marrow shows high expression.
Immunohistochemistry-Paraffin: Band 3 Antibody [NBP2-49409] - Staining in human bone marrow and cerebral cortex tissues using anti-SLC4A1 antibody. Corresponding SLC4A1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
Ankyrin Brain
Antibody
NBP2-33863
Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
TPD52 Antibody
NBP2-38952
Immunohistochemistry-Paraffin: TPD52 Antibody [NBP2-38952] - Staining of human prostate shows high expression.
Immunohistochemistry-Paraffin: TPD52 Antibody [NBP2-38952] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: TPD52 Antibody [NBP2-38952] - Staining in human prostate and skeletal muscle tissues using anti-TPD52 antibody. Corresponding TPD52 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Pancreatic
Polypeptide/PP Antibo
NB100-1793
Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Pancreas with antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunocytochemistry/Immunofluorescence: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Histologic comparison of wild type and Seriola dumerili mouse pancreata. Seriola dumerili Ins2 mice (bottom) have normal islet morphology and cyto-architecture compared to littermates with endogenous mouse Ins 1 and Ins 2 (top); pancreatic polypeptide (green; B,E). Scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-40768-3) licensed under a CC-BY licence.
(3 Reviews)
(16 Publications)
Species: Hu, Pm, Mu, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
Polycystin-1/PKD1
Antibody [Unconjugate ...
AF6270
Polycystin‑1/PKD1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human Polycystin‑1/PKD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6270) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membranes, cytoplasm and endoplasmic reticula. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Species: Hu
Applications: ICC
Titin Antibody
NBP1-88071
Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Analysis in human skeletal muscle and liver tissues. Corresponding TTN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human fallopian tube shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Titin Antibody [NBP1-88071] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
(3 Publications)
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
Ankyrin 3 Antibody
NBP3-05549
Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Ankyrin 3 Antibody, CPCA-ANK3, at a dilution of 1:2,000 in green, and costained with mouse mAb to RBFOX3/NeuN at a dilution of 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The Ankyrin 3 antibody stains the axonal initial segments, while the RBFOX3/NeuN antibody reveals perikarya and nuclei of neurons.
Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.
Species: Bv, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, WB
Alkaline
Phosphatase/ALPL Anti ...
AF2910
Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF2910) at 2 µg/mL overnight at 4 °C. Tissue was stained red and counterstained with DAPI (blue). Image courtesy of Paul J. Simmons, Ph.D., Director, Center for Stem Cell Research, The University of Texas Health Science Center at Houston, Houston, Texas, USA. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
(2 Reviews)
(18 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, IP, WB
gamma-Synuclein
Antibody (JM90-32)
NBP2-66861
Flow Cytometry: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of Hela cells with gamma Synuclein antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Immunocytochemistry/Immunofluorescence: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Staining gamma Synuclein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: gamma-Synuclein Antibody (JM90-32) [NBP2-66861] - Analysis of paraffin-embedded human breast cancer tissue using anti-gamma Synuclein antibody. Counter stained with hematoxylin.
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TRPA1 Antibody
NB110-40763
Immunocytochemistry/Immunofluorescence: TRPA1 Antibody [NB110-40763] - Analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells.
Immunohistochemistry: TRPA1 Antibody [NB110-40763] - Staining TRPA1 in mouse intestine.
Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
(2 Reviews)
(45 Publications)
Species: Gp, Hu, Mu, Rt, Ze
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NM23-H1 Antibody
(1D7)
H00004830-M02
ELISA: NM23-H1 Antibody (1D7) [H00004830-M02] - Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.
Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 10 ug/ml.
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
EPB41 Antibody
(3D9)
H00002035-M01
Western Blot: EPB41 Antibody (3D9) [H00002035-M01] - Analysis of EPB41 expression in transfected 293T cell line by EPB41 monoclonal antibody (M01), clone 3D9.Lane 1: EPB41 transfected lysate(81 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: EPB41 Antibody (3D9) [H00002035-M01] - Analysis of monoclonal antibody to EPB41 on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: EPB41 Antibody (3D9) [H00002035-M01] - Analysis of monoclonal antibody to EPB41 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB

Publications for Ankyrin 1 Antibody (NBP1-71805)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
All Applications
Filter By Species
Human
(2)
All Species

Review for Ankyrin 1 Antibody (NBP1-71805) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-71805:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Ankyrin 1 NBP1-71805
Enlarge 		4
reviewed by:
Verified Customer
 WB Human 05/20/2015
View

Summary

ApplicationWestern Blot
Sample Testedhuman a549
SpeciesHuman
Lota1

Blocking

Blocking Details5% milk

Primary Anitbody

Dilution Ratio1/1000 in 5% milk 4C overnight

Secondary Antibody

Secondary DescriptionLi-Cor Donkey anti-rabbit IR800CW
Secondary Concentration1/15000

Details

Detection NotesLi-Cor odyssey scanner. 2 bands visible.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ankyrin 1 Antibody (NBP1-71805) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional Ankyrin 1 Products

Bioinformatics Tool for Ankyrin 1 Antibody (NBP1-71805)

Discover related pathways, diseases and genes to Ankyrin 1 Antibody (NBP1-71805). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ankyrin 1 Antibody (NBP1-71805)

Discover more about diseases related to Ankyrin 1 Antibody (NBP1-71805).
 

Pathways for Ankyrin 1 Antibody (NBP1-71805)

View related products by pathway.

PTMs for Ankyrin 1 Antibody (NBP1-71805)

Learn more about PTMs related to Ankyrin 1 Antibody (NBP1-71805).

Blogs on Ankyrin 1

There are no specific blogs for Ankyrin 1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0
Western Blot Ankyrin 1 NBP1-71805
Enlarge
 Verified Customer
05/20/2015
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol ANK1
Entrez
Uniprot