Trypsin 1/PRSS1 Antibody [Unconjugated]

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Trypsin 1/PRSS1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Trypsin 1/PRSS1 Monoclonal Antibody (Catalog # MAB3848) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Trypsin 1/PRSS1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trypsin 1/PRSS1
Ala16-Ser247
Accession # P07477
Specificity
Detects human Trypsin 1/PRSS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Trypsin 2 and rhTrypsin 3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PRSS1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF3848 in the following applications:

Publications
Read Publication using
AF3848 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trypsin 1/PRSS1 Antibody [Unconjugated]

  • Beta-trypsin
  • Cationic trypsinogen
  • digestive zymogen
  • DKFZp779A0837
  • EC 3.4.21
  • EC 3.4.21.4
  • FLJ57296
  • MGC120175
  • MGC149362
  • nonfunctional trypsin 1
  • protease, serine, 1 (trypsin 1)
  • PRSS1
  • PTRYI
  • Serine protease 1
  • TRP1
  • TRY1beta-trypsin
  • TRY4
  • Trygn16
  • TRYP1
  • Trypsin 1
  • Trypsin I
  • trypsin-1
  • Trypsinogen 1
  • trypsinogen A

Background

Human Trypsin 1, encoded by the PRSS1 gene, is also known as Cationic Trypsinogen (1). Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes (2). Mutations in the PRSS1 gene can cause hereditary pancreatitis (3).

  1. Emi, M. et al. (1986) Gene 41:305.
  2. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1483, Academic Press, San Diego.
  3. Teich, N. et al. (2006) Hum. Mutat. 27:721.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Trypsin 1/PRSS1 Antibody (AF3848)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-Fr.


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(1)
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Reviews for Trypsin 1/PRSS1 Antibody (AF3848) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Human and Rat.

Reviews using AF3848:
Filter by Applications
IHC-Fr
(1)
WB
(1)
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Mouse
(1)
Human and Rat
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Trypsin 1/PRSS1 AF3848
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reviewed by:
Scott Messenger
IHC-Fr Mouse 12/15/2017
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse pancreas in OCT
SpeciesMouse
Lotzhv021705a
Western Blot Trypsin 1/PRSS1 AF3848
Enlarge
reviewed by:
Scott Messenger
WB Human and Rat 05/19/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuamn acini,rat acini,AR42J rat acinar-like cell
SpeciesHuman and Rat

Comments

Commentswhole cell lysates 50 ug. 12% gel. 1/1000 dilution of primary antibody

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Recent Reviews

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1
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Scott Messenger
12/15/2017
Application: IHC-Fr
Species: Mouse

Scott Messenger
05/19/2017
Application: WB
Species: Human and Rat

Bioinformatics

Gene Symbol PRSS1
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