TROP-2 Antibody [Allophycocyanin]

mIMCD-3 mouse epithelial cell line was stained with Goat Anti-Mouse TROP-2 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1122A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

TROP-2 Antibody [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
  Ser82-Thr268
  Accession # Q8BGV3
• Specificity: Detects mouse TROP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human TROP-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TACSTD2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TROP-2 Antibody [Allophycocyanin]

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

TROP-2, also named tumor-associated calcium signal transducer 2 (TACSTD2), GA733 tumor associated antigen, and epithelial glycoprotein-1 (EGP-1), is a type I transmembrane protein highly expressed in carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family.  The other family member is GA7332, also called EpCAM, TROP1, 171A, gp40 and KSA.  TROP2 can transduce an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (FAB1122A)(4)

Publications using FAB1122A

• Jiří Švec, Monika Šťastná, Lucie Janečková, Dušan Hrčkulák, Martina Vojtěchová, Jakub Onhajzer et al. TROP2 Represents a Negative Prognostic Factor in Colorectal Adenocarcinoma and Its Expression Is Associated with Features of Epithelial–Mesenchymal Transition and Invasiveness Cancers (Basel) 8/26/2022 [PMID: 36077674]
• Laura Crowley, Francesco Cambuli, Luis Aparicio, Maho Shibata, Brian D Robinson, Shouhong Xuan et al. A single-cell atlas of the mouse and human prostate reveals heterogeneity and conservation of epithelial progenitors eLife 9/11/2020 [PMID: 32915138]
• Preston D. Crowell, Jonathan J. Fox, Takao Hashimoto, Johnny A. Diaz, Héctor I. Navarro, Gervaise H. Henry et al. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate Cell Reports 8/6/2019 [PMID: 31390564]
• Stoyanova T, Goldstein A, Cai H, Drake J, Huang J, Witte O Regulated proteolysis of Trop2 drives epithelial hyperplasia and stem cell self-renewal via beta-catenin signaling. Genes Dev, 2012-10-15;26(20):2271-85. 2012-10-15 [PMID: 23070813] (ICC, Mouse)

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • Q8BGV3()