TROP-2 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

TROP-2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
  Ser82-Thr268
  Accession # Q8BGV3
• Specificity: Detects mouse TROP-2 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) TROP-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TACSTD2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TROP-2 Antibody [Biotin]

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

TROP-2 is alternatively known as tumor-associated calcium signal transducer 2 and lymphocyte antigen 97.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (BAF1122)(4)

Publications using BAF1122

• JD Barros-Sil, DE Linn, I Steiner, G Guo, A Ali, H Pakula, G Ashton, I Peset, M Brown, NW Clarke, RT Bronson, GC Yuan, SH Orkin, Z Li, E Baena Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer Cell Rep, 2018-12-18;25(12):3504-3518.e6. 2018-12-18 [PMID: 30566873] (Flow Cytometry, Mouse)
• Stoyanova T, Goldstein A, Cai H, Drake J, Huang J, Witte O Regulated proteolysis of Trop2 drives epithelial hyperplasia and stem cell self-renewal via beta-catenin signaling. Genes Dev, 2012-10-15;26(20):2271-85. 2012-10-15 [PMID: 23070813] (Immunoprecipitation, Western Blot, Mouse)
• Rita U. Lukacs, Andrew S. Goldstein, Devon A. Lawson, Donghui Cheng, Owen N. Witte Isolation, cultivation, and characterization of adult murine prostate stem cells Nature Protocols 2010-04-03 [PMID: 20360765]
• Goldstein AS, Lawson DA, Cheng D, Sun W, Garraway IP, Witte ON Trop2 identifies a subpopulation of murine and human prostate basal cells with stem cell characteristics. Proc. Natl. Acad. Sci. U.S.A., 2008-12-16;105(52):20882-7. 2008-12-16 [PMID: 19088204] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • Q8BGV3()