TROP-2 Antibody [Biotin]

TROP‑2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Goat Anti-Human TROP‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF650) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

TROP-2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TROP-2
  Thr88-Thr274
  Accession # P09758
• Specificity: Detects human TROP-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human E‑Cadherin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TACSTD2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TROP-2 Antibody [Biotin]

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (BAF650)(3)

Publications using BAF650

• Liu, S;Hawley, SJ;Kunder, CA;Hsu, EC;Shen, M;Westphalen, L;Auman, H;Newcomb, LF;Lin, DW;Nelson, PS;Feng, Z;Tretiakova, MS;True, LD;Vakar-Lopez, F;Carroll, PR;Simko, J;Gleave, ME;Troyer, DA;McKenney, JK;Brooks, JD;Liss, MA;Stoyanova, T; High expression of Trop2 is associated with aggressive localized prostate cancer and is a candidate urinary biomarker Scientific reports 2024-01-04 [PMID: 38177207] (ELISA Detection, Western Blot, IHC, Human)
• H Lee, M Choi, J Lim, M Jo, JY Han, TM Kim, Y Cho Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers Theranostics, 2018-01-01;8(2):505-517. 2018-01-01 [PMID: 29290823] (Functional Assay)
• Goldstein AS, Lawson DA, Cheng D, Sun W, Garraway IP, Witte ON Trop2 identifies a subpopulation of murine and human prostate basal cells with stem cell characteristics. Proc. Natl. Acad. Sci. U.S.A., 2008-12-16;105(52):20882-7. 2008-12-16 [PMID: 19088204] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • P09758()