TROP-2 Antibody [Unconjugated]

mIMCD-3 mouse epithelial cell line was stained with Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

TROP-2 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using Goat Anti-Mouse TROP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TROP‑2 was detected in immersion fixed paraffin-embedded sections of mouse skin using Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Gene expression analysis of Pten mutant prostates.(A) i) Heatmap demonstrating that control samples (C1-C5) show similar expression patterns to each other, as do mutant samples (M1-M5). ii) Genes chosen for validation are indicated in heatmap derived from the average of control and mutant samples. Red indicates genes that are upregulated in mutants (116 genes), relative to controls, and green indicates genes that are downregulated in the mutants relative to controls (91 genes). (B) Quantitative RTPCR validation of indicated androgen-dependent genes differentially expressed in mutants and controls (Clu p = 0.001, Trop2 p = 0.002, Nkx3.1 p = 0.02, and Pbsn p = 0.003). mRNA accumulation was normalized to GAPDH. (C) Differential antibody staining of CLU, TROP2, and PBSN at P10 validated microarray results. (D) Whole mount in situ hybridization of Nkx3.1 shows a decrease in expression in P10 mutant prostates. The anterior prostate (AP), dorsal lateral prostate (DL) and urethra (UR) are indicated. Red arrows indicate presence of stain in the controls and absence in the mutant. “*” denotes statistical significance. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0129470), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pten deletion does not lead to an accumulation of progenitor cells.Antibody staining on sections of P0 and P7 prostates with antibodies to CLU and TROP2 shows no accumulation of these proteins at earlier stages of development in controls (A, B, C, D). Increased expression of these markers is seen in the mutant (E, F, G, H) relative to controls from P7. Red arrowheads indicate buds. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0129470), licensed under a CC-BY license. Not internally tested by R&D Systems.

Gene expression analysis of Pten mutant prostates.(A) i) Heatmap demonstrating that control samples (C1-C5) show similar expression patterns to each other, as do mutant samples (M1-M5). ii) Genes chosen for validation are indicated in heatmap derived from the average of control and mutant samples. Red indicates genes that are upregulated in mutants (116 genes), relative to controls, and green indicates genes that are downregulated in the mutants relative to controls (91 genes). (B) Quantitative RTPCR validation of indicated androgen-dependent genes differentially expressed in mutants and controls (Clu p = 0.001, Trop2 p = 0.002, Nkx3.1 p = 0.02, and Pbsn p = 0.003). mRNA accumulation was normalized to GAPDH. (C) Differential antibody staining of CLU, TROP2, and PBSN at P10 validated microarray results. (D) Whole mount in situ hybridization of Nkx3.1 shows a decrease in expression in P10 mutant prostates. The anterior prostate (AP), dorsal lateral prostate (DL) and urethra (UR) are indicated. Red arrows indicate presence of stain in the controls and absence in the mutant. “*” denotes statistical significance. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0129470), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TROP-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
  Ser82-Thr268
  Accession # Q8BGV3
• Specificity: Detects mouse TROP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant human TROP‑2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TACSTD2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TROP-2 Antibody [Unconjugated]

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

TROP-2, also named tumor-associated calcium signal transducer 2 (TACSTD2), GA733 tumor associated antigen, and epithelial glycoprotein-1 (EGP-1), is a type I transmembrane protein highly expressed in carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family.  The other family member is GA7332, also called EpCAM, TROP1, 171A, gp40 and KSA.  TROP2 can transduce an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (AF1122)(28)

Publications using AF1122

• D Li, Y Zhan, N Wang, F Tang, CJ Lee, G Bayshtok, AR Moore, EWP Wong, MR Pachai, Y Xie, J Sher, JL Zhao, M Khudoynaza, A Gopalan, J Chan, E Khurana, P Shepherd, NM Navone, P Chi, Y Chen ETV4 mediates dosage-dependent prostate tumor initiation and cooperates with p53 loss to generate prostate cancer Science Advances, 2023-04-05;9(14):eadc9446. 2023-04-05 [PMID: 37018402] (IHC, Mouse)
• Jiří Švec, Monika Šťastná, Lucie Janečková, Dušan Hrčkulák, Martina Vojtěchová, Jakub Onhajzer et al. TROP2 Represents a Negative Prognostic Factor in Colorectal Adenocarcinoma and Its Expression Is Associated with Features of Epithelial–Mesenchymal Transition and Invasiveness Cancers (Basel) 8/26/2022 [PMID: 36077674]
• J Silva, F Alkan, S Ramalho, G Snieckute, S Prekovic, AK Garcia, S Hernández-, R van der Ka, D Barnum, L Hoekman, M Altelaar, W Zwart, SJE Suijkerbui, S Bekker-Jen, WJ Faller Ribosome impairment regulates intestinal stem cell identity via ZAK? activation Nature Communications, 2022-08-02;13(1):4492. 2022-08-02 [PMID: 35918345] (IHC, Mouse, Transgenic Mouse)
• MA Abu El Maa, J Terzic, C Keime, D Rovito, R Lutzing, D Yanushko, M Parisotto, E Grelet, IJ Namer, V Lindner, G Laverny, D Metzger Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression Science Advances, 2022-07-22;8(29):eabo2295. 2022-07-22 [PMID: 35867798] (IHC, Mouse)
• Emma Laporte, Florian Hermans, Silke De Vriendt, Annelies Vennekens, Diether Lambrechts, Charlotte Nys et al. Decoding the activated stem cell phenotype of the neonatally maturing pituitary eLife 6/14/2022 [PMID: 35699412]
• Su-Hyung Lee, Bogun Jang, Jimin Min, Ela W. Contreras-Panta, Kimberly S. Presentation, Alberto G. Delgado et al. Up-regulation of Aquaporin 5 Defines Spasmolytic Polypeptide-Expressing Metaplasia and Progression to Incomplete Intestinal Metaplasia Cellular and Molecular Gastroenterology and Hepatology 1/1/2022 [PMID: 34455107]
• Mohamed A. Abu el Maaty, Elise Grelet, Céline Keime, Anna-Isavella Rerra, Justine Gantzer, Camille Emprou et al. Single-cell analyses unravel cell type–specific responses to a vitamin D analog in prostatic precancerous lesions Science Advances 7/30/2021 [PMID: 34330705]
• Renaud Mevel, Ivana Steiner, Susan Mason, Laura CA Galbraith, Rahima Patel, Muhammad ZH Fadlullah et al. RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development eLife 10/7/2020 [PMID: 33025905]
• Katherine M. Riera, Bogun Jang, Jimin Min, Joseph T. Roland, Qing Yang, William T. Fesmire et al. Trop2 is upregulated in the transition to dysplasia in the metaplastic gastric mucosa The Journal of Pathology 7/1/2020 [PMID: 32432338]
• CJ Aros, MK Paul, CJ Pantoja, B Bisht, LK Meneses, P Vijayaraj, JM Sandlin, B France, JA Tse, MW Chen, DW Shia, TM Rickabaugh, R Damoiseaux, BN Gomperts High-Throughput Drug Screening Identifies a Potent Wnt Inhibitor that Promotes Airway Basal Stem Cell Homeostasis Cell Rep, 2020-02-18;30(7):2055-2064.e5. 2020-02-18 [PMID: 32075752] (Flow Cytometry, Mouse)
• Wang Y, Chiang IL, Ohara TE et al. Long-Term Culture Captures Injury-Repair Cycles of Colonic Stem Cells Cell 11/14/2019 [PMID: 31708126]
• K Mahiddine, A Blaisdell, S Ma, A Créquer-Gr, CA Lowell, A Erlebacher Relief of tumor hypoxia unleashes the tumoricidal potential of neutrophils J. Clin. Invest., 2020-01-02;0(0):. 2020-01-02 [PMID: 31600172] (IHC-P, Mouse)
• Preston D. Crowell, Jonathan J. Fox, Takao Hashimoto, Johnny A. Diaz, Héctor I. Navarro, Gervaise H. Henry et al. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate Cell Reports 8/6/2019 [PMID: 31390564]
• Kieran M Short, Alexander N Combes, Valerie Lisnyak, James G Lefevre, Lynelle K Jones, Melissa H Little et al. Branching morphogenesis in the developing kidney is not impacted by nephron formation or integration eLife 7/31/2018 [PMID: 30063208]
• TJ Lynch, PJ Anderson, PG Rotti, SR Tyler, AK Crooke, SH Choi, DT Montoro, CL Silverman, W Shahin, R Zhao, CW Jensen-Cod, A Adamcakova, TIA Evans, W Xie, Y Zhang, H Mou, BP Herring, PS Thorne, J Rajagopal, C Yeaman, KR Parekh, JF Engelhardt Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium Cell Stem Cell, 2018-04-12;0(0):. 2018-04-12 [PMID: 29656941] (ICC, Mouse)
• S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration Cell Stem Cell, 2017-12-14;22(1):35-49.e7. 2017-12-14 [PMID: 29249464] (IHC, Mouse)
• James G Lefevre, Kieran M. Short, Timothy O. Lamberton, Odyssé Michos, Daniel Graf, Ian M. Smyth et al. Branching morphogenesis in the developing kidney is governed by rules that pattern the ureteric tree Development 4/28/2017 [PMID: 29038307]
• James G Lefevre, Kieran M. Short, Timothy O. Lamberton, Odyssé Michos, Daniel Graf, Ian M. Smyth et al. Branching morphogenesis in the developing kidney is governed by rules that pattern the ureteric tree Development 2017-04-28 [PMID: 29038307] (Immunohistochemistry, Transgenic Mouse)
• Maternal Fat Feeding Augments Offspring Nephron Endowment in Mice PLoS ONE, 2016-08-22;11(8):e0161578. 2016-08-22 [PMID: 27547968] (IHC-Fr, Mouse)
• Lokody I, Francis J, Gardiner J, Erler J, Swain A Pten Regulates Epithelial Cytodifferentiation during Prostate Development. PLoS ONE, 2015-06-15;10(6):e0129470. 2015-06-15 [PMID: 26076167] (IHC-P, Mouse)
• Short K, Combes A, Lefevre J, Ju A, Georgas K, Lamberton T, Cairncross O, Rumballe B, McMahon A, Hamilton N, Smyth I, Little M Global quantification of tissue dynamics in the developing mouse kidney. Dev Cell, 2014-04-28;29(2):188-202. 2014-04-28 [PMID: 24780737] (IHC, Mouse)
• Sekiya S, Suzuki A Hepatocytes, rather than cholangiocytes, can be the major source of primitive ductules in the chronically injured mouse liver. Am J Pathol, 2014-03-01;184(5):1468-78. 2014-03-01 [PMID: 24594350] (IHC, Mouse)
• Taguchi , Keiko, Hirano , Ikuo, Itoh , Tohru, Tanaka , Minoru, Miyajima , Atsushi, Suzuki , Akira, Motohashi , Hozumi, Yamamoto , Masayuki Nrf2 enhances cholangiocyte expansion in Pten-deficient livers. Mol Cell Biol, 2013-12-30;34(5):900-13. 2013-12-30 [PMID: 24379438] (IHC, Mouse)
• JIANYE YANG, ZHAOHUI ZHU, HONGFEI WANG, FEIFEI LI, XINLING DU, RUNLIN Z. MA Trop2 regulates the proliferation and differentiation of murine compact-bone derived MSCs International Journal of Oncology 9/1/2013 [PMID: 23783522]
• Jianbo Wang, Kaihua Zhang, Dorota Grabowska, Aimin Li, Yiyu Dong, Ryan Day et al. Loss of Trop2 Promotes Carcinogenesis and Features of Epithelial to Mesenchymal Transition in Squamous Cell Carcinoma Molecular Cancer Research 12/1/2011 [PMID: 21970857]
• Jianbo Wang, Kaihua Zhang, Dorota Grabowska, Aimin Li, Yiyu Dong, Ryan Day et al. Loss of Trop2 Promotes Carcinogenesis and Features of Epithelial to Mesenchymal Transition in Squamous Cell Carcinoma Molecular Cancer Research 2011-12-01 [PMID: 21970857] (Western Blot, Mouse)
• Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells. J. Invest. Dermatol., 2011-06-16;131(10):2049-57. 2011-06-16 [PMID: 21677668] (Flow Cytometry, IHC, Mouse)
• Rafael Cubas, Sheng Zhang, Min Li, Changyi Chen, Qizhi Yao Trop2 expression contributes to tumor pathogenesis by activating the ERK MAPK pathway Molecular Cancer 12/1/2010 [PMID: 20858281]

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • Q8BGV3()