TRMT5 Antibody (OTI1B5) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI1B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

TRMT5 Antibody (OTI1B5) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-303 of human TRMT5(NP_065861) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRMT5
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: >95%

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TRMT5 Antibody (OTI1B5) [CoraFluor™ 1]

• KIAA1393EC 2.1.1.31
• M1G-methyltransferase
• TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae)
• TRM5MGC111453
• tRNA (guanine-N(1)-)-methyltransferase
• tRNA [GM37] methyltransferase
• tRNA methyltransferase 5
• tRNA-N1G37 methyltransferase

Background

tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 (PubMed 15248782)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRMT5