TRMT12 Antibody

Images

 
Western Blot: TRMT12 Antibody [NBP1-76583] - Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry: TRMT12 Antibody [NBP1-76583] - Immunocytochemistry of TYW2 in K562 cells with TYW2 antibody at 10 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TRMT12 Antibody Summary

Immunogen
Antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2. The immunogen is located within the first 50 amino acids of TYW2.
Specificity
TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRMT12
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry 10 ug/ml
Application Notes
TYW2 antibody can be used for detection of TYW2 by Western blot at 0.5 - 1 mg/mL.
Control Peptide
TRMT12 Peptide (NBP1-76583PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRMT12 Antibody

  • Alpha-amino-alpha-carboxypropyl transferase TYW2
  • EC 2.1.1
  • EC 2.1.1.32
  • EC 2.5.1.-
  • FLJ20772
  • homolog of yeast tRNA methyltransferase
  • TRM12
  • tRNA methyltranferase 12 homolog (S. cerevisiae)
  • tRNA methyltranferase 12 homolog
  • tRNA methyltransferase 12 homolog (S. cerevisiae)
  • tRNA-yW synthesizing protein 2
  • tRNA-yW-synthesizing protein 2
  • TYW2tRNA wybutosine-synthesizing protein 2 homolog

Background

TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRMT12
Uniprot