QTRT1 Antibody - BSA Free

Western Blot: QTRT1 Antibody [NBP2-20030] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: QTRT1 Antibody [NBP2-20030] - QTRT1 antibody detects QTRT1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: QTRT1 protein stained by QTRT1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: QTRT1 Antibody [NBP2-20030] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: QTRT1 Antibody [NBP2-20030] - Immunohistochemical analysis of paraffin-embedded Muscle, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

QTRT1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit QTRT1 Antibody - BSA Free (NBP2-20030) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human QTRT1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: QTRT1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (83%), Zebrafish (81%), Xenopus tropicalis (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for QTRT1 Antibody - BSA Free

• EC 2.4.2.29
• Guanine insertion enzyme
• queuine tRNA-ribosyltransferase 1
• queuine tRNA-ribosyltransferase
• TGTFP3235
• TGUT
• tRNA-guanine transglycosylase

Background

tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: QTRT1
• Uniprot:
  • Q9BXR0(Human)