TRIM25 Antibody (5B5B12) - BSA Free

Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded rectum cancer tissues using TRIM25 mouse mAb with DAB staining.

Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)

Western Blot: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.

Immunohistochemistry: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of paraffin-embedded bladder cancer tissues using TRIM25 mouse mAb with DAB staining.

ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA: TRIM25 Antibody (5B5B12) [NBP2-61814] - Analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 5B5B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRIM25 Antibody (5B5B12) - BSA Free Summary

• Description: Novus Biologicals Mouse TRIM25 Antibody (5B5B12) - BSA Free (NBP2-61814) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-TRIM25 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human TRIM25 (AA: 211-360) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM25
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 68.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for TRIM25 Antibody (5B5B12) - BSA Free

• EC 6.3.2.19
• EC 6.3.2.n3
• EFP
• EFPZ147
• Estrogen-responsive finger protein
• RING finger protein 147
• RNF147
• RNF147E3 ubiquitin/ISG15 ligase TRIM25
• TRIM25
• tripartite motif containing 25
• tripartite motif-containing 25
• Tripartite motif-containing protein 25
• Ubiquitin/ISG15-conjugating enzyme TRIM25
• zinc finger protein 147 (estrogen-responsive finger protein)
• Zinc finger protein 147
• zinc finger protein-147
• ZNF147
• ZNF147tripartite motif protein TRIM25

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM25 Antibody (NBP2-61814)(1)

Publication using NBP2-61814

• King KE, Ghosh P, Wozniak AL. et Al. TRIM25 dictates selective miRNA loading into extracellular vesicles during inflammation Sci Rep 2023-12-22 [PMID: 38135735]

Bioinformatics

• Gene Symbol: TRIM25