TYW1 Antibody

Western Blot: TYW1 Antibody [NBP2-20772] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: TYW1 Antibody [NBP2-20772] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

• Reactivity: Hu, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TYW1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human TYW1. The exact sequence is proprietary.
• Predicted Species: Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TYW1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (89%), Zebrafish (84%), Xenopus laevis (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for TYW1 Antibody

• FLJ10900
• MGC23001
• MGC60291
• Radical S-adenosyl methionine and flavodoxin domain-containing protein 1
• radical S-adenosyl methionine and flavodoxin domains 1
• RSAFD1
• tRNA wybutosine-synthesizing protein 1 homolog
• tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
• tRNA-yW synthesizing protein 1 homolog A
• tRNA-yW-synthesizing protein
• TYW1A
• YPL207W

Background

Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TYW1