LCMT2 Antibody

Images

 
Western Blot: LCMT2 Antibody [NBP1-76585] - Rat brain tissue lysate with LCMT2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: LCMT2 Antibody [NBP1-76585] - Immunofluorescence of LCMT2 in mouse brain tissue with LCMT2 antibody at 20 ug/mL.
Immunohistochemistry: LCMT2 Antibody [NBP1-76585] - Immunohistochemistry of TYW4 in mouse brain tissue with TYW4 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LCMT2 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human TYW4. The immunogen is located within the first 50 amino acids of TYW4.
Specificity
TYW4 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW4 are known to exist; this antibody will detect only the long isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LCMT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
LCMT2 Peptide (NBP1-76585PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LCMT2 Antibody

  • EC 2.1.1.-
  • FLJ51321
  • KIAA0547tRNA-Wybutosine-synthesizing protein 4
  • leucine carboxyl methyltransferase 2
  • MGC9534
  • p21WAF1/CIP1 promoter-interacting protein
  • PPM2
  • tRNA wybutosine-synthesizing protein 4 homolog
  • tRNA yW-synthesizing protein 4 homolog
  • TYW4tRNA-yW synthesizing protein 4

Background

TYW4 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW4 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LCMT2
Uniprot