LCMT2 Antibody - BSA Free

Western Blot: LCMT2 Antibody [NBP1-76585] - Rat brain tissue lysate with LCMT2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry: LCMT2 Antibody [NBP1-76585] - Immunohistochemistry of TYW4 in mouse brain tissue with TYW4 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: LCMT2 Antibody - BSA Free [NBP1-76585] - Immunofluorescence of TYW1 in mouse brain tissue with TYW1 antibody at 20 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LCMT2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LCMT2 Antibody - BSA Free (NBP1-76585) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human TYW4. The immunogen is located within the first 50 amino acids of TYW4. Amino Acid Squence: VQNTNDSSALSKRSLA
• Specificity: TYW4 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW4 are known to exist; this antibody will detect only the long isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LCMT2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  LCMT2 Peptide (NBP1-76585PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for LCMT2 Antibody - BSA Free

• EC 2.1.1.-
• FLJ51321
• KIAA0547tRNA-Wybutosine-synthesizing protein 4
• leucine carboxyl methyltransferase 2
• MGC9534
• p21WAF1/CIP1 promoter-interacting protein
• PPM2
• tRNA wybutosine-synthesizing protein 4 homolog
• tRNA yW-synthesizing protein 4 homolog
• TYW4tRNA-yW synthesizing protein 4

Background

TYW4 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW4 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LCMT2
• Uniprot:
  • NP_055608(Human)