TRIOBP Antibody

Images

 
Western Blot: TRIOBP Antibody [NBP2-20726] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: TRIOBP Antibody [NBP2-20726] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: TRIOBP Antibody [NBP2-20726] - Human lung cancer diluted at 1:500
Western Blot: TRIOBP Antibody [NBP2-20726] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: TRIOBP Antibody [NBP2-20726] - Immunohistochemical analysis of paraffin-embedded MDA-MB-157 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TRIOBP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Tara. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm; cytoskeleton
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIOBP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
261 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIOBP Antibody

  • FLJ39315
  • TARA
  • TRIO and F-actin binding protein
  • TRIO and F-actin-binding protein

Background

This gene encodes a protein with an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. The protein interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility and cell growth. The protein also associates with F-actin and stabilizes F-actin structures. Mutations in this gene have been associated with a form of autosomal recessive nonsyndromic deafness. Multiple alternatively spliced transcript variants that would encode different isoforms have been found for this gene, however some transcripts may be subject to nonsense-mediated decay (NMD). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIOBP
Uniprot