TRIOBP Antibody - BSA Free

Immunohistochemistry-Paraffin: TRIOBP Antibody [NBP2-20726] - Human lung cancer diluted at 1:500

Immunocytochemistry/Immunofluorescence: TRIOBP Antibody [NBP2-20726] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Western Blot: TRIOBP Antibody [NBP2-20726] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIOBP antibody [C2C3-2], C-term (NBP2-20726) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: TRIOBP Antibody [NBP2-20726] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIOBP antibody [C2C3-2], C-term (NBP2-20726) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRIOBP Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRIOBP Antibody - BSA Free (NBP2-20726) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRIOBP. The exact sequence is proprietary.
• Localization: Nucleus; Cytoplasm; cytoskeleton
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIOBP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 261 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRIOBP Antibody - BSA Free

• FLJ39315
• TARA
• TRIO and F-actin binding protein
• TRIO and F-actin-binding protein

Background

This gene encodes a protein with an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. The protein interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility and cell growth. The protein also associates with F-actin and stabilizes F-actin structures. Mutations in this gene have been associated with a form of autosomal recessive nonsyndromic deafness. Multiple alternatively spliced transcript variants that would encode different isoforms have been found for this gene, however some transcripts may be subject to nonsense-mediated decay (NMD). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TRIOBP
• Uniprot:
  • Q9H2D6(Human)