PINCH1/LIMS1 Antibody (591604)

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Western blot shows lysates of A549 human lung carcinoma cell line, WI‑38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
591604
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

PINCH1/LIMS1 Antibody (591604) Summary

Immunogen
E. coli-derived recombinant human PINCH1
Ala2-Gly108
Accession # P48059
Specificity
Detects human, mouse, and rat PINCH1 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LIMS1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB6257 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PINCH1/LIMS1 Antibody (591604)

  • LIM and senescent cell antigen-like domains 1
  • LIM and senescent cell antigen-like-containing domain protein 1
  • LIMS1
  • PINCH1
  • PINCH-1
  • PINCHPINCH1Particularly interesting new Cys-His protein 1
  • Renal carcinoma antigen NY-REN-48

Background

Particularly interesting new Cys-His protein 1 (PINCH1), also known as LIMS1 (LIM and senescent cell antigen-like-containing domain protein 1), is a 37‑40 kDa, widely expressed LIM-only protein. Human PINCH1 is 325 amino acids (aa) in length and contains a tandem array of five LIM domains. Within the region used as an immunogen, human PINCH1 shares 100% and 99% aa sequence identity with mouse and rat PINCH1, respectively. PINCH1 is an effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PINCH1/LIMS1 Antibody (MAB6257)(1)

We have publications tested in 1 confirmed species: Zebrafish.

We have publications tested in 1 application: WB.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LIMS1
Entrez
Uniprot