PINCH1/LIMS1 Antibody (591604)

Western blot shows lysates of A549 human lung carcinoma cell line, WI-38 human lung fibroblast cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PINCH1 Monoclonal Antibody (Catalog # MAB6257) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PINCH1 at approximately 37-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 591604
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PINCH1/LIMS1 Antibody (591604) Summary

• Immunogen: E. coli-derived recombinant human PINCH1
  Ala2-Gly108
  Accession # P48059
• Specificity: Detects human, mouse, and rat PINCH1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIMS1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PINCH1/LIMS1 Antibody (591604)

• LIM and senescent cell antigen-like domains 1
• LIM and senescent cell antigen-like-containing domain protein 1
• LIMS1
• PINCH1
• PINCH-1
• PINCHPINCH1Particularly interesting new Cys-His protein 1
• Renal carcinoma antigen NY-REN-48

Background

Particularly interesting new Cys-His protein 1 (PINCH1), also known as LIMS1 (LIM and senescent cell antigen-like-containing domain protein 1), is a 37‑40 kDa, widely expressed LIM-only protein. Human PINCH1 is 325 amino acids (aa) in length and contains a tandem array of five LIM domains. Within the region used as an immunogen, human PINCH1 shares 100% and 99% aa sequence identity with mouse and rat PINCH1, respectively. PINCH1 is an effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PINCH1/LIMS1 Antibody (MAB6257)(1)

Publication using MAB6257

• Meder B, Huttner IG, Sedaghat-Hamedani F, Just S, Dahme T, Frese KS, Vogel B, Kohler D, Kloos W, Rudloff J, Marquart S, Katus HA, Rottbauer W PINCH proteins regulate cardiac contractility by modulating integrin-linked kinase-protein kinase B signaling. Mol. Cell. Biol., 2011-06-13;31(16):3424-35. 2011-06-13 [PMID: 21670146] (Western Blot, Zebrafish)

Bioinformatics

• Gene Symbol: LIMS1
• Uniprot:
  • P48059(Human)