ch-TOG Antibody - Azide and BSA Free

Western Blot: ch-TOG Antibody [NB500-182] - Specificity of polyclonal antibodies against ch-TOGp. Total cell protein (50 mg) from human HeLa cells was immunoblotted with a rabbit polyclonal serum generated against the C-terminal 301 amino acids of the ch-TOGp.

• Reactivity: Hu, Mu, Rt, Ma
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ch-TOG Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit ch-TOG Antibody - Azide and BSA Free (NB500-182) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-ch-TOG Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A recombinant segment of the C-terminal domain of human ch-TOG [UniProt# Q14008]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CKAP5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000
• Application Notes: A band at 200 kDa can be detected by Western blot.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 200 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for ch-TOG Antibody - Azide and BSA Free

• CHTOG
• Ch-TOG
• Colonic and hepatic tumor over-expressed gene protein
• cytoskeleton associated protein 5
• cytoskeleton-associated protein 5
• EC 2.7.7.6
• EC 3.1.1.29
• KIAA0097FLJ35359
• MSPS
• TOG
• TOGp

Background

Ch-TOG (colonic and hepatic tumor overexpressed gene protein), also called cytoskeleton-associated protein 5 (CKAP5) is a member of TOG/XMAP215 family which associates with the plus end of microtubules and regulates microtubule dynamics/organization. Ch-TOG promotes cytoplasmic microtubule nucleation and elongation, and plays a major role in organizing spindle poles. The N-terminal half of Ch-TOG contains a microtubule-binding domain whereas the C-terminal half contains a KXGS motif for binding the tubulin dimers. In spindle formation, Ch-TOG protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. Moreover, Ch-TOG is essential to proper interaction of microtubules with the cell cortex for directional cell movement. Ch-TOG also implicates in translation of MBP-mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2. Ch-TOG interacts with SLAIN2 also and can be detected on centrosomes during interphase or on the spindle poles as well as microtubules during mitosis. Ch-TOG is expressed in several normal tissues including skeletal muscle, brain, heart, placenta, lung, liver, kidney, pancreas etc. and is found overexpressed in hepatomas and colonic cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ch-TOG Antibody (NB500-182)(2)

Publications using NB500-182

• Kourtidis A, Anastasiadis PZ. PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications. Cell Cycle. 2016-01-29 [PMID: 26822694] (ICC/IF, Human)
• Dionne, MA et al. ch-TOGp is required for microtubule aster formation in a mammalian mitotic extract. J Biol Chem. 275(16):12346-52. 2000-04-21 [PMID: 10766876] (ICC/IF, WB, IP, Human)

Bioinformatics

• Gene Symbol: CKAP5
• Entrez:
  • 9793(Human)
  • 75786(Mouse)
  • 311191(Rat)
• Uniprot:
  • Q14008(Human)