ch-TOG Antibody

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Western Blot: ch-TOG Antibody [NB500-182] - Specificity of polyclonal antibodies against ch-TOGp. Total cell protein (50 mg) from human HeLa cells was immunoblotted with a rabbit polyclonal serum generated against the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ch-TOG Antibody Summary

Immunogen
A recombinant segment of the C-terminal domain of human ch-TOG [UniProt# Q14008]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CKAP5
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
This ch-TOG antibody may be used for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot. A band at 200 kDa can be detected by Western blot.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB500-182 in the following applications:

  • 2 publications
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Human, mammal, mouse, rat

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for ch-TOG Antibody

  • CHTOG
  • Ch-TOG
  • Colonic and hepatic tumor over-expressed gene protein
  • cytoskeleton associated protein 5
  • cytoskeleton-associated protein 5
  • EC 2.7.7.6
  • EC 3.1.1.29
  • KIAA0097FLJ35359
  • MSPS
  • TOG
  • TOGp

Background

Ch-TOG (colonic and hepatic tumor overexpressed gene protein), also called cytoskeleton-associated protein 5 (CKAP5) is a member of TOG/XMAP215 family which associates with the plus end of microtubules and regulates microtubule dynamics/organization. Ch-TOG promotes cytoplasmic microtubule nucleation and elongation, and plays a major role in organizing spindle poles. The N-terminal half of Ch-TOG contains a microtubule-binding domain whereas the C-terminal half contains a KXGS motif for binding the tubulin dimers. In spindle formation, Ch-TOG protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. Moreover, Ch-TOG is essential to proper interaction of microtubules with the cell cortex for directional cell movement. Ch-TOG also implicates in translation of MBP-mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2. Ch-TOG interacts with SLAIN2 also and can be detected on centrosomes during interphase or on the spindle poles as well as microtubules during mitosis. Ch-TOG is expressed in several normal tissues including skeletal muscle, brain, heart, placenta, lung, liver, kidney, pancreas etc. and is found overexpressed in hepatomas and colonic cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ch-TOG Antibody (NB500-182)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(2)
IP
(1)
WB
(1)
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Human
(2)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CKAP5
Entrez
Uniprot