CEP68 Antibody - BSA Free

Western Blot: CEP68 Antibody [NBP1-82988] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Immunocytochemistry/Immunofluorescence: CEP68 Antibody [NBP1-82988] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus, microtubule organizing center & cell junctions.

Immunohistochemistry-Paraffin: CEP68 Antibody [NBP1-82988] - Staining of human fallopian tube.

Immunohistochemistry-Paraffin: CEP68 Antibody [NBP1-82988] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: CEP68 Antibody [NBP1-82988] - Staining of human fallopian tube, kidney, liver and lymph node using Anti-CEP68 antibody NBP1-82988 (A) shows similar protein distribution across tissues to independent antibody NBP1-82989 (B).

Immunohistochemistry-Paraffin: CEP68 Antibody [NBP1-82988] - Staining of human lymph node.

Immunohistochemistry-Paraffin: CEP68 Antibody [NBP1-82988] - Staining of human kidney.

Immunohistochemistry-Paraffin: CEP68 Antibody [NBP1-82988] - Staining of human liver.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CEP68 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CEP68 Antibody - BSA Free (NBP1-82988) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SGLLKTRPSLPARLDRWPFSDPDVEGQLPRKGGEQGKESLVQCVKTFCCQLEELICWLYNVADVTDHGTAARSNLTSLKSSLQLYRQFKKDIDEHQSLTESVLQKGEILLQCLLENTPV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CEP68
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CEP68 Protein (NBP1-82988PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CEP68 Antibody - BSA Free

• centrosomal protein 68kDa
• centrosomal protein of 68 kDa
• Cep68
• FLJ36750
• KIAA0582FLJ25920

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CEP68 Antibody (NBP1-82988) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Jerrold Turner ICC Mouse 08/16/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: Adult small intestine
• Species: Mouse
• Lot: R36873

Comments

• Comments: We tested the antibody on smears (cytology-like preps) of mouse small intestinal epithelial cells fixed with 2% paraformaldehyde/lysine/periodate (PLP; McLean and Nakane. J Histochem Cytochem, 1974) or-20°C methanol. After permeabilizing with 0.1% saponin (PLP-fixed only) and blocking with 5% donkey serum (in PBS), slides were incubated with antibodies at the dilutions indicated below and a rat monoclonal anti-ZO-1 (tight junction protein) antibody (clone R40.76) for 2 hours at room temperature. After washing, species-specific secondary antibodies (cross-adsorbed) produced in goat were applied. ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: CEP68