TRIM27 Antibody

Western Blot: TRIM27 Antibody [NBP2-20712] - Sample (30 ug of whole cell lysate) A: NT2D1 B: SK-N-SH 7.5% SDS PAGE diluted at 1:1000

Immunohistochemistry-Paraffin: TRIM27 Antibody [NBP2-20712] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRIM27 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRIM27. The exact sequence is proprietary.
• Localization: Nucleus (By similarity); Cytoplasm (By similarity)
• Predicted Species: Mouse (100%), Rat (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM27
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRIM27 Antibody

• Ret finger proteinRING finger protein 76
• RFPtripartite motif protein TRIM27
• RNF76RFP transforming protein
• tripartite motif containing 27
• tripartite motif-containing 27
• Tripartite motif-containing protein 27
• zinc finger protein RFP

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TRIM27
• Uniprot:
  • P14373(Human)