TRIM27 Antibody

Images

 
Western Blot: TRIM27 Antibody [NBP2-20711] - Expression in transfected 293T cell line by TRIM27 polyclonal antibody. A: Non-transfected lysate. B: TRIM27 transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: TRIM27 Antibody [NBP2-20711] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TRIM27 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.66 mg/ml

Order Details

TRIM27 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TRIM27. The exact sequence is proprietary.
Localization
Nucleus (By similarity); Cytoplasm (By similarity)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM27
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-20711 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.66 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM27 Antibody

  • Ret finger proteinRING finger protein 76
  • RFPtripartite motif protein TRIM27
  • RNF76RFP transforming protein
  • tripartite motif containing 27
  • tripartite motif-containing 27
  • Tripartite motif-containing protein 27
  • zinc finger protein RFP

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRIM27 Antibody (NBP2-20711)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIM27
Uniprot