TRIM27 Antibody - BSA Free

Western Blot: TRIM27 Antibody [NBP2-20711] - TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody diluted at a dilution of 1:500.

Immunocytochemistry/Immunofluorescence: TRIM27 Antibody [NBP2-20711] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TRIM27 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Western Blot: TRIM27 Antibody [NBP2-20711] - TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody diluted by 1:500.

Western Blot: TRIM27 Antibody [NBP2-20711] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody diluted at 1:500.

Immunoprecipitation: TRIM27 Antibody [NBP2-20711] - Immunoprecipitation of TRIM27 protein from Jurkat whole cell extracts using 5 ug of TRIM27 antibody. Western blot analysis was performed using TRIM27 antibody.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRIM27 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRIM27. The exact sequence is proprietary.
• Localization: Nucleus (By similarity); Cytoplasm (By similarity)
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM27
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TRIM27 Antibody - BSA Free

• Ret finger proteinRING finger protein 76
• RFPtripartite motif protein TRIM27
• RNF76RFP transforming protein
• tripartite motif containing 27
• tripartite motif-containing 27
• Tripartite motif-containing protein 27
• zinc finger protein RFP

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM27 Antibody (NBP2-20711)(1)

Publication using NBP2-20711

• Kahle JJ, Gulbahce N, Shaw CA et al. Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia. Hum Mol Genet 2011-02-01 [PMID: 21078624] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: TRIM27
• Uniprot:
  • P14373(Human)