TRIM25 Antibody - BSA Free

Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis the level of TRIM25 and GRP78 in HCT116 and Huh7 cells treated with TM (5 ug/ml) or TG (1 uM) for 12 h. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP2-20710] - HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: TRIM25 protein stained by TRIM25 antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um

Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP2-20710] - Human colon carcinoma, using TRIM25 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: TRIM25 Antibody [NBP2-20710] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody diluted at 1:500.

Western Blot: TRIM25 Antibody [NBP2-20710] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunoprecipitation: TRIM25 Antibody [NBP2-20710] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TRIM25 protein by 2 ug of TRIM25 antibody 7.5% SDS-PAGE The immunoprecipitated TRIM25 protein was detected by TRIM25 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Western Blot: TRIM25 Antibody [NBP2-20710] - TRIM25 functions as a feedback mechanism that responds to ER stress in tumor cells. Western blot analysis of UPR signaling, including phospho-JNK/JNK and phospho-eIF2alpha/eIF2alpha in HCT116 cells after stable knockdown of TRIM25 in HCT116 cells. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-019-14190-2) licensed under a CC-BY license.

Immunohistochemistry: TRIM25 Antibody [NBP2-20710] - Knockdown of TRIM25 in HCC suppresses tumor growth in nude mice.a–c Huh7 cells stably expressing the indicated shRNAs (shNC, shTRIM25-1 & shTRIM25-2) were subcutaneously injected in nude mice respectively. Shown are average tumor volumes over time (n = 8) (a) & representative image (b) & weights (c) of tumors at day 25. d Kaplan–Meier analysis of overall survival percentage in nude mice inoculated with Huh7-shNC or Huh7-shTRIM25. The statistical significance was assessed using two-sided log-rank test according to mice with depletion of control or TRIM25. e, f Representative images (e) of IHC staining & the relative IHC scores (n = 8) (f) of TRIM25, Ki67, Keap1 & Nrf2 in HCC tissues of mice inoculated with Huh7-shNC or Huh7-shTRIM25-1&-2. Scale bar, 50 μm. g, h Representative IHC staining images (g) & statistical data (h) of TRIM25, Keap1 & Nrf2 expression in HCC tissues (TRIM25 low expression group, n = 45 & TRIM25 high expression group, n = 45). Scale bar, 50 μm. For a, c, f, & h, data represent the mean ± SEM. Statistical significance was assessed using two-tailed Student’s t-tests. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31953436), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRIM25 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRIM25. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM25
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRIM25 Antibody - BSA Free

• EC 6.3.2.19
• EC 6.3.2.n3
• EFP
• EFPZ147
• Estrogen-responsive finger protein
• RING finger protein 147
• RNF147
• RNF147E3 ubiquitin/ISG15 ligase TRIM25
• TRIM25
• tripartite motif containing 25
• tripartite motif-containing 25
• Tripartite motif-containing protein 25
• Ubiquitin/ISG15-conjugating enzyme TRIM25
• zinc finger protein 147 (estrogen-responsive finger protein)
• Zinc finger protein 147
• zinc finger protein-147
• ZNF147
• ZNF147tripartite motif protein TRIM25

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TRIM25 Antibody (NBP2-20710)(1)

Publication using NBP2-20710

• Liu Y, Tao S, Liao L et al. TRIM25 promotes the cell survival and growth of hepatocellular carcinoma through targeting Keap1-Nrf2 pathway Nat Commun 2020-01-17 [PMID: 31953436]

Bioinformatics

• Gene Symbol: TRIM25
• Uniprot:
  • Q14258(Human)