TRIM25 Antibody


Western Blot: TRIM25 Antibody [NBP2-20710] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP2-20710] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
1 mg/ml

Order Details

TRIM25 Antibody Summary

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 243 and 491 (Q14258) of TRIM25
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7).
0.01% Thimerosal
1 mg/ml
Immunogen affinity purified


Application Notes
suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
71 kDa

Reactivity Notes


Alternate Names for TRIM25 Antibody

  • EC
  • EC 6.3.2.n3
  • EFPZ147
  • Estrogen-responsive finger protein
  • RING finger protein 147
  • RNF147E3 ubiquitin/ISG15 ligase TRIM25
  • tripartite motif containing 25
  • tripartite motif-containing 25
  • Tripartite motif-containing protein 25
  • Ubiquitin/ISG15-conjugating enzyme TRIM25
  • zinc finger protein 147 (estrogen-responsive finger protein)
  • Zinc finger protein 147
  • zinc finger protein-147
  • ZNF147tripartite motif protein TRIM25


The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


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Contact Information

Product PDFs


Gene Symbol TRIM25

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