TRIM25 Antibody

Western Blot: TRIM25 Antibody [NBP1-00113] - Detection of Human TRIM25 on HeLa whole cell lysate using NBP1-00113. TRIM25 was also immunoprecipitated by rabbit anti-TRIM25 antibodies NBP1-00114 and NBP1-00115.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TRIM25 Antibody Summary

• Description: Novus Biologicals Rabbit TRIM25 Antibody (NBP1-00113) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human tripartite motif-containing 25 using the numbering given in entry NP_005073.2 (GeneID 7706).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM25
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000-1:10000
• Control:

  TRIM25 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM25 Antibody

• EC 6.3.2.19
• EC 6.3.2.n3
• EFP
• EFPZ147
• Estrogen-responsive finger protein
• RING finger protein 147
• RNF147
• RNF147E3 ubiquitin/ISG15 ligase TRIM25
• TRIM25
• tripartite motif containing 25
• tripartite motif-containing 25
• Tripartite motif-containing protein 25
• Ubiquitin/ISG15-conjugating enzyme TRIM25
• zinc finger protein 147 (estrogen-responsive finger protein)
• Zinc finger protein 147
• zinc finger protein-147
• ZNF147
• ZNF147tripartite motif protein TRIM25

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM25
• Entrez:
  • 7706(Human)
• Uniprot:
  • NP_005073.2(Human)