TRIB1 Antibody (OTI8C8) - Azide and BSA Free

Images

 
Western Blot: TRIB1 Antibody (OTI8C8) - Azide and BSA Free [NBP2-74596] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB1.
Immunohistochemistry: TRIB1 Antibody (OTI8C8) - Azide and BSA Free [NBP2-74596] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: TRIB1 Antibody (OTI8C8) - Azide and BSA Free [NBP2-74596] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI8C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

TRIB1 Antibody (OTI8C8) - Azide and BSA Free Summary

Description
Novus Biologicals Mouse TRIB1 Antibody (OTI8C8) - Azide and BSA Free (NBP2-46205) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
Full length human recombinant protein of human TRIB1 (NP_079471) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TRIB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TRIB1 Antibody (OTI8C8) - Azide and BSA Free

  • C8FWG-protein-coupled receptor induced protein
  • GIG-2
  • GIG2G-protein-coupled receptor-induced protein 2
  • G-protein-coupled receptor-induced gene 2 protein
  • SKIP1
  • TRB-1
  • TRB1phosphoprotein regulated by mitogenic pathways
  • tribbles homolog 1 (Drosophila)
  • tribbles homolog 1
  • tribbles-like protein 1

Background

Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIB1