MAP4K5 Antibody - BSA Free

Western Blot: MAP4K5 Antibody [NBP1-31234] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE, diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: MAP4K5 Antibody [NBP1-31234] - Analysis of A431, using MAP4K5 antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: MAP4K5 Antibody [NBP1-31234] - SAS xenograft, using KHS antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: MAP4K5 Antibody [NBP1-31234] - Immunoprecipitation of KHS protein from 293T whole cell extracts using 5 ug of KHS antibody. Western blot analysis was performed using KHS antibody.EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MAP4K5 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human KHS. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Mouse (94%), Rat (94%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP4K5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MAP4K5 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• GCKR
• germinal center kinase-related
• KHS1
• KHSMEKKK 5
• Kinase homologous to SPS1/STE20
• MAP4K5
• MAPK/ERK kinase kinase kinase 5
• MAPKKKK5
• MEK kinase kinase 5
• mitogen-activated protein kinase kinase kinase kinase 5

Background

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MAP4K5
• Entrez:
  • 11183(Human)
• Uniprot:
  • Q9Y4K4()