MAP4K5 Antibody

Images

 
Western Blot: MAP4K5 Antibody [NBP1-31234] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: MAP4K5 Antibody [NBP1-31234] - Analysis of A431, using MAP4K5 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MAP4K5 Antibody [NBP1-31234] - SAS xenograft, using KHS antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: MAP4K5 Antibody [NBP1-31234] - Immunoprecipitation of KHS protein from 293T whole cell extracts using 5 ug of KHS antibody. Western blot analysis was performed using KHS antibody.EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MAP4K5 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human KHS. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Mouse (94%), Rat (94%), Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP4K5
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for MAP4K5 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • GCKR
  • germinal center kinase-related
  • KHS1
  • KHSMEKKK 5
  • Kinase homologous to SPS1/STE20
  • MAP4K5
  • MAPK/ERK kinase kinase kinase 5
  • MAPKKKK5
  • MEK kinase kinase 5
  • mitogen-activated protein kinase kinase kinase kinase 5

Background

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP4K5
Entrez
Uniprot