TRF-2 Antibody (689524) [Alexa Fluor® 350]

• Applications: WB
• Clone: 689524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

TRF-2 Antibody (689524) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human TRF2
  Gln78-Thr238 (predicted)
  Accession # Q15554
• Specificity: Detects human TRF2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TRF1 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

TRF2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55-60 kDa, ubiquitously expressed nuclear protein that participates in telomere homeostasis. TRF-2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway. It also collaborates with TRF1 to promote normal telomere length. Human TRF2 is 500 amino acids (aa) in length. It contains an N-terminal Arg-rich region (aa 13-30), a dimerization domain (aa 46-112), an NLS (aa 329-333), and a DNA binding HTH myb-type domain (aa 442-499). There is one potential alternate start site 42 aa upstream of the standard start site, and one splice form that shows a 13 aa substitution for aa 239-500. Over aa 78-238, human TRF2 shares 96% aa identity with mouse TRF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TRF-2 Antibody (FAB5635U). (Showing 1 - 3 of 3 FAQ).

1. Are the TRF-2 antibodies validated in Simple Western?
   • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
2. Does TRF-2 antibodies comes in lyophilized form?
   • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
3. What the theoretical molecular weight for TRF-2 antibodies?
   • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.