Reactivity | Hu, Mu, Rt, Ma, MaSpecies Glossary |
Applications | WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, CyTOF-ready |
Clone | 4A794.15 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 1 mg/ml |
Description | The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain. |
Immunogen | Baculovirus expressed whole length TRF2 protein was used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643). |
Marker | Telomeres marker |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | TERF2 |
Purity | Protein G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | TRF-2 may be detected as a single band or as a doublet in Western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. Observed molecular weights could vary depending on molecular weight standards used and gel conditions. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Proximity Ligation Assay reported in scientific literature (PMID 27366950). Use in immunoblotting reported in scientific literature (PMID: 23708666). |
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Theoretical MW | 55.55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.05% Sodium Azide |
Concentration | 1 mg/ml |
Purity | Protein G purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Fernanda gutierrez rodrigues |
WB | Human | 09/08/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NB100-56506AF350 | |
Alexa Fluor 405 | NB100-56506AF405 | |
Alexa Fluor 488 | NB100-56506AF488 | |
Alexa Fluor 532 | NB100-56506AF532 | |
Alexa Fluor 594 | NB100-56506AF594 | |
Alexa Fluor 647 | NB100-56506AF647 | |
Alexa Fluor 700 | NB100-56506AF700 | |
Alexa Fluor 750 | NB100-56506AF750 | |
Allophycocyanin | NB100-56506APC | |
Biotin | NB100-56506B | |
DyLight 350 | NB100-56506UV | |
DyLight 405 | NB100-56506V | |
DyLight 488 | NB100-56506G | |
DyLight 550 | NB100-56506R | |
DyLight 594 | NB100-56506DL594 | |
DyLight 650 | NB100-56506C | |
DyLight 680 | NB100-56506FR | |
DyLight 755 | NB100-56506IR | |
FITC | NB100-56506F | |
HRP | NB100-56506H | |
Janelia Fluor 549 | NB100-56506JF549 | |
Janelia Fluor 646 | NB100-56506JF646 | |
PE | NB100-56506PE | |
PerCP | NB100-56506PCP |
Diseases for TRF-2 Antibody (NB100-56506)Discover more about diseases related to TRF-2 Antibody (NB100-56506).
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PTMs for TRF-2 Antibody (NB100-56506)Learn more about PTMs related to TRF-2 Antibody (NB100-56506).
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Application Highlight: Recent uses of TERF2 in immunofluorescence (IF) Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion. TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th... Read full blog post. |
TRF2: Telomere Maintenance and Protection TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an... Read full blog post. |
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Fernanda gutierrez rodrigues 09/08/2017 |
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Application: | WB | |
Species: | Human |