TRF-2 Antibody (4A794.15)

Images

 
Simple Western: TRF-2 Antibody (4A794.15) [NB100-56506] - Simple Western lane view shows a specific band for TRF2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more
Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis in human Jurkat cell lysate at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Staining of TRF2-bound telomeres in human HeLa cells (Courtesy of Fotiadou, et al, 2004).
Immunohistochemistry-Paraffin: TRF-2 Antibody (4A794.15) [NB100-56506] - Formalin-fixed, paraffin-embedded transitional cell carcinoma, urinary bladder, stained with TRF2 antibody (4 ug/ml), peroxidase-conjugate and DAB ...read more
Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Intracellular flow cytometric analysis of TRF2 in 10^6 human Jurkat cells using 0.1 ug of NB100-56506. The shaded histogram represents cells alone, blue ...read more
Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Total protein from mouse 3T3 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis using the Alexa Fluor (R) 647 conjugate. Staining of TRF2 in 10^6 human Jurkat cells using 10 ul (0.1 ug). The blue histogram represents isotype control ...read more
Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56506. Staining of TRF2 in 10^6 human Jurkat cells using 10 ul (0.1 ug) of this antibody. The blue ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, CyTOF-ready
Clone
4A794.15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

TRF-2 Antibody (4A794.15) Summary

Immunogen
Baculovirus expressed whole length TRF2 protein was used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).
Marker
Telomeres marker
Specificity
The recognizes distinct doublet bands of approximate size 66 kDa corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TERF2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Simple Western 1:50
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • ELISA 2 ug/ml
  • Flow Cytometry 0.1 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2 ug/10^6 cells
  • Proximity Ligation Assay
  • CyTOF-ready
Application Notes
1. TLR2 may be detected as a single band or as a doublet in western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. However, observed molecular weights could vary depending on molecular weight standards used and gel conditions. 2. The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. 3. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Refer to the published literature for additional information. 4. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Proximity Ligation Assay reported in scientific literature (PMID 27366950)
Theoretical MW
55.55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
TRF-2 Lysate (NBL1-16815)
Reviewed Applications
Read 1 Review rated 4
using
NB100-56506 in the following applications:

Publications
Read Publications using
NB100-56506 in the following applications:

  • 6 publications
  • 28 publications
  • IF
    1 publication
  • 1 publication
  • IP
    4 publications
  • PLA
    2 publications
  • WB
    27 publications
  • 1 publication
  • WP
    1 publication

Reactivity Notes

Muntjac (deer)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TRF-2 Antibody (4A794.15)

  • 4A794 trf2
  • 4A794
  • 4A794.15 trf2
  • 4A794.15
  • anti-trf2 4A794
  • anti-trf2 4A794.15
  • clone 4A794
  • clone 4A794.15
  • Telomeric DNA-binding protein
  • telomeric repeat binding factor 2
  • Telomeric repeat binding protein 2
  • telomeric repeat-binding factor 2
  • TERF 2
  • TERF2
  • TRBF 2
  • TRBF2
  • TRF 2
  • TRF2
  • TRF-2
  • TTAGGG repeat binding factor 2
  • TTAGGG repeat-binding factor 2

Background

TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-56506
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, CyTOF-ready
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Publications for TRF-2 Antibody (NB100-56506)(92)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Muntjac (deer).

We have publications tested in 9 applications: ChIP, ICC/IF, IF, IHC-P, IP, PLA, WB, WB,IF,IP, WP.


Filter By Application
ChIP
(6)
ICC/IF
(28)
IF
(1)
IHC-P
(1)
IP
(4)
PLA
(2)
WB
(27)
WB,IF,IP
(1)
WP
(1)
All Applications
Filter By Species
Human
(42)
Mouse
(4)
Rat
(1)
Muntjac (deer)
(1)
All Species
Showing Publications 1 - 10 of 92. Show All 92 Publications.
Publications using NB100-56506 Applications Species
Jahn A, Rane G, Paszkowski-Rogacz M et al. ZBTB48 is both a vertebrate telomere-binding protein and a transcriptional activator. EMBO Rep. May 12 2017 [PMID: 28500257] (ICC/IF, Human) ICC/IF Human
Jeitany M, Bakhos-Douaihy D, Silvestre DC et al. Opposite effects of GCN5 and PCAF knockdowns on the alternative mechanism of telomere maintenance. Oncotarget Apr 18 2017 [PMID: 28412741] (ICC/IF, PLA, Human) ICC/IF, PLA Human
Fouquerel E, Lormand J, Bose A et al. Oxidative guanine base damage regulates human telomerase activity. Nat. Struct. Mol. Biol. Dec 1 2016 [PMID: 27820808] (ICC/IF, Human) ICC/IF Human
Konishi A, Izumi T, Shimizu S. TRF2 Interacts with Core Histones to Stabilize Chromosome Ends J. Biol. Chem. Aug 11 2016 [PMID: 27514743] (WP, IP, ICC/IF, Mouse, Human) WP, IP, ICC/IF Mouse, Human
Picco V, Coste I, Giraud-Panis MJ et al. ERK1/2/MAPK pathway-dependent regulation of the telomeric factor TRF2 Oncotarget. Jul 19 2016 [PMID: 27366950] (PLA, Human) PLA Human
Kim YH, Kim KT, Lee SJ et al. Image-aided Suicide Gene Therapy Utilizing Multifunctional hTERT-targeting Adenovirus for Clinical Translation in Hepatocellular Carcinoma. Theranostics. 2016 Jan 06 [PMID: 26909111] (ICC/IF, Human) ICC/IF Human
Hasegawa D, Okabe S, Okamoto K et al. G-quadruplex ligand-induced DNA damage response coupled with telomere dysfunction and replication stress in glioma stem cells Biochem. Biophys. Res. Commun. Feb 26 2016 [PMID: 26845351] (ICC/IF, Human) ICC/IF Human
Kocak H. Functional Analysis of ACD Mutations in Dyskeratosis Congenita Thesis. 2014 (ChIP, Human) ChIP Human
Shukla S, Schmidt JC, Goldfarb KC et al. Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects. Nat Struct Mol Biol 2016 Apr [PMID: 26950371]
Altieri P, Barisione C, Lazzarini E et al. Testosterone Antagonizes Doxorubicin-Induced Senescence of Cardiomyocytes. J Am Heart Assoc. 2016 Jan 09 [PMID: 26746999] (WB, Mouse, Rat)

Details:
TRF-2 antibody clone 4A794.15 was used for WB analysis in experiments involving C57BL/6 murine primary neonatal cardiomyocytes and rat embryonic cardiac cell line H9c2.
WB Mouse, Rat
Show All 92 Publications.

Review for TRF-2 Antibody (NB100-56506) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56506:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot TRF-2 NB100-56506
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reviewed by:
Fernanda gutierrez rodrigues
WB Human 09/08/2017
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Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman
LotAB070705A-4

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TRF-2 Antibody (NB100-56506). (Showing 1 - 4 of 4 FAQs).

  1. Are the TRF-2 antibodies validated in Simple Western?
    • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
  2. Does TRF-2 antibodies comes in lyophilized form?
    • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
  3. What the theoretical molecular weight for TRF-2 antibodies?
    • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.
  4. What is the immunogen sequence of this TRF-2 antibody?
    • The whole length of Baculovirus expressed TRF-2 protein.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56506AF405
Alexa Fluor 488 Labeled NB100-56506AF488
Alexa Fluor 647 Labeled NB100-56506AF647
Alexa Fluor 700 Labeled NB100-56506AF700
Allophycocyanin Labeled NB100-56506APC
Biotin Labeled NB100-56506B
DyLight 350 Labeled NB100-56506UV
DyLight 405 Labeled NB100-56506V
DyLight 488 Labeled NB100-56506G
DyLight 550 Labeled NB100-56506R
DyLight 650 Labeled NB100-56506C
DyLight 680 Labeled NB100-56506FR
DyLight 755 Labeled NB100-56506IR
HRP Labeled NB100-56506H
PE Labeled NB100-56506PE
PerCP Labeled NB100-56506PCP

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Blogs on TRF-2.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

TRF2: Telomere Maintenance and Protection
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an...  Read full blog post.

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Recent Reviews

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Fernanda gutierrez rodrigues
09/08/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol TERF2
Entrez
Uniprot