TRF-2 Antibody (4A794.15)

Simple Western: TRF-2 Antibody (4A794.15) [NB100-56506] - Simple Western lane view shows a specific band for TRF2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis in human Jurkat cell lysate at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Staining of TRF2-bound telomeres in human HeLa cells (Courtesy of Fotiadou, et al, 2004).

Immunohistochemistry-Paraffin: TRF-2 Antibody (4A794.15) [NB100-56506] - Formalin-fixed, paraffin-embedded transitional cell carcinoma, urinary bladder, stained with TRF2 antibody (4 ug/ml), peroxidase-conjugate and DAB chromogen. Note specific nuclear staining. Tumor/normal adjacent tissue array slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Intracellular flow cytometric analysis of TRF2 in 10^6 human Jurkat cells using 0.1 ug of NB100-56506. The shaded histogram represents cells alone, blue represents isotype control and red represents NB100-56506, anti-TRF2.

Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Total protein from mouse 3T3 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TRF2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TRF-2 (4A794.15) conjugated to FITC [NB100-56506F] at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis using the Alexa Fluor (R) 647 conjugate. Staining of TRF2 in 10^6 human Jurkat cells using 10 ul (0.1 ug). The blue histogram represents isotype control and red represents anti-TRF2.

Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56506. Staining of TRF2 in 10^6 human Jurkat cells using 10 ul (0.1 ug) of this antibody. The blue histogram represents isotype control and red represents this antibody, anti-TRF2.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, CyTOF-ready
• Clone: 4A794.15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TRF-2 Antibody (4A794.15) Summary

• Immunogen: Baculovirus expressed whole length TRF2 protein was used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).
• Marker: Telomeres marker
• Specificity: The recognizes distinct doublet bands of approximate size 66 kDa corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TERF2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4 ug/ml
  • Simple Western 1:50
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • ELISA 2 ug/ml
  • Flow Cytometry 0.1 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2 ug/10^6 cells
  • Proximity Ligation Assay
  • CyTOF-ready
• Application Notes: 1. TLR2 may be detected as a single band or as a doublet in western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. However, observed molecular weights could vary depending on molecular weight standards used and gel conditions. 2. The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. 3. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Refer to the published literature for additional information. 4. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Proximity Ligation Assay reported in scientific literature (PMID 27366950)
• Theoretical MW: 55.55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  TRF-2 Lysate (NBL1-16815)

Reactivity Notes

Muntjac (deer)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for TRF-2 Antibody (4A794.15)

• 4A794 trf2
• 4A794
• 4A794.15 trf2
• 4A794.15
• anti-trf2 4A794
• anti-trf2 4A794.15
• clone 4A794
• clone 4A794.15
• Telomeric DNA-binding protein
• telomeric repeat binding factor 2
• Telomeric repeat binding protein 2
• telomeric repeat-binding factor 2
• TERF 2
• TERF2
• TRBF 2
• TRBF2
• TRF 2
• TRF2
• TRF-2
• TTAGGG repeat binding factor 2
• TTAGGG repeat-binding factor 2

Background

TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRF-2 Antibody (NB100-56506)(92)

Publications using NB100-56506

• Jahn A, Rane G, Paszkowski-Rogacz M et al. ZBTB48 is both a vertebrate telomere-binding protein and a transcriptional activator. EMBO Rep. May 12 2017 [PMID: 28500257] (ICC/IF, Human)
• Jeitany M, Bakhos-Douaihy D, Silvestre DC et al. Opposite effects of GCN5 and PCAF knockdowns on the alternative mechanism of telomere maintenance. Oncotarget Apr 18 2017 [PMID: 28412741] (ICC/IF, PLA, Human)
• Fouquerel E, Lormand J, Bose A et al. Oxidative guanine base damage regulates human telomerase activity. Nat. Struct. Mol. Biol. Dec 1 2016 [PMID: 27820808] (ICC/IF, Human)
• Konishi A, Izumi T, Shimizu S. TRF2 Interacts with Core Histones to Stabilize Chromosome Ends J. Biol. Chem. Aug 11 2016 [PMID: 27514743] (WP, IP, ICC/IF, Mouse, Human)
• Kocak H. Functional Analysis of ACD Mutations in Dyskeratosis Congenita Thesis. 2014 (ChIP, Human)
• Picco V, Coste I, Giraud-Panis MJ et al. ERK1/2/MAPK pathway-dependent regulation of the telomeric factor TRF2 Oncotarget. Jul 19 2016 [PMID: 27366950] (PLA, Human)
• Shukla S, Schmidt JC, Goldfarb KC et al. Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects. Nat Struct Mol Biol 2016 Apr [PMID: 26950371]
• Kim YH, Kim KT, Lee SJ et al. Image-aided Suicide Gene Therapy Utilizing Multifunctional hTERT-targeting Adenovirus for Clinical Translation in Hepatocellular Carcinoma. Theranostics. 2016 Jan 06 [PMID: 26909111] (ICC/IF, Human)
• Hasegawa D, Okabe S, Okamoto K et al. G-quadruplex ligand-induced DNA damage response coupled with telomere dysfunction and replication stress in glioma stem cells Biochem. Biophys. Res. Commun. Feb 26 2016 [PMID: 26845351] (ICC/IF, Human)
• Altieri P, Barisione C, Lazzarini E et al. Testosterone Antagonizes Doxorubicin-Induced Senescence of Cardiomyocytes. J Am Heart Assoc. 2016 Jan 09 [PMID: 26746999] (WB, Mouse, Rat)
  
  Details:
  TRF-2 antibody clone 4A794.15 was used for WB analysis in experiments involving C57BL/6 murine primary neonatal cardiomyocytes and rat embryonic cardiac cell line H9c2.
• Xi L, Schmidt JC, Zaug AJ et al. A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biol. 2015 Nov 10 [PMID: 26553065] (ICC/IF, Human)
• Saquilabon Cruz GM, Kong X, Silva BA et al. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites. Nucleic Acids Res 2016 Feb 18 [PMID: 26424850]
• Clynes D, Jelinska C, Xella B et al. Suppression of the alternative lengthening of telomere pathway by the chromatin remodelling factor ATRX Nat Commun. 2015 Jul 06 [PMID: 26143912] (ICC/IF, Human)
  
  Details:
  TRF2 antibody clone 4A794.15 (Imgenex IMG-124A) was used for ICC-IF in experiments involving U2OS(ATRX) cells and the immunostaining assay implicated fixation in 4% paraformaldehyde, prepermeabilization with ice cold 0.5% Triton X-100 for 5?min, use of primary antibody at 1:200 dilution, detection using Alexa conjugated secondary antibodies (Figure 1d, Figure 3, Figure 5, Supplementary Figure 1d). ICC-IF was performed on HeLa cells also which were exposed or not to Pyridostatin/PDS, a highly selective G4-interacting ligand (Supplementary Figure 4b).
• Burla R, Carcuro M, Raffa GD et al. AKTIP/Ft1, a New Shelterin-Interacting Factor Required for Telomere Maintenance PLoS Genet. 2015 Jun 01 [PMID: 26110528] (WB, Human)
• Panneer Selvam S, De Palma RM, Oaks JJ et al. Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation Sci Signal. 2015 Jun 17 [PMID: 26082434]
• Fujita T, Yuno M, Okuzaki D et al. Identification of Non-Coding RNAs Associated with Telomeres Using a Combination of enChIP and RNA Sequencing PLoS ONE 2015 Apr 16 [PMID: 25874893] (ICC/IF, Human)
• Schertzer M, Jouravleva K, Perderiset M et al. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA Nucleic Acids Res. 2015 Jan 27 [PMID: 25628358] (ICC/IF, Human)
• Salvati E, Rizzo A, Iachettini S et al. A basal level of DNA damage and telomere deprotection increases the sensitivity of cancer cells to G-quadruplex interactive compounds Nucleic Acids Res. 2015 Jan 23 [PMID: 25618850] (WB, ChIP, Human)
  
  Details:
  TRF2 antibody used for WB on lysates of BJ-hTERT fibroblasts cells expressing shGFP, shP53, shRb or BJ-EHLT (Figure 5G), and on BJ-hTERT fibroblasts transfected with siRNA-GFP and siRNA-TRF2 (Figure 6A). WB also performed on lysates of cell lines namely - BJ, BJ-hTERT, BJ-EHLT, BJ-EHLT/ Ras (Figure S5c). The antibody also used for ChIP assay on BJ-hTERT cells treated or not with 1 or 5 uM of RHPS4, G-quadruplex/G4 ligand for 24 hours (Figure 2F).
• Freund A, Zhong Fl, Venteicher As et al. Proteostatic Control of Telomerase Function through TRiC-Mediated Folding of TCAB1 Cell et al. 2014 Nov 20 [PMID: 25467444] (ICC/IF, Human)
  
  Details:
  TRF2 antibody used for ICC-IF at 1.7 ug/ml concentration on cells transfected some siRnAs (siDKC1, siTCAB1, siCCT1, siCCT2, siCCT4) and HA-TERT and TERC plasmids (Figure 3A)
• Kocak H, Ballew BJ, Bisht K et al. Hoyeraal-Hreidarsson syndrome caused by a germline mutation in the TEL patch of the telomere protein TPP1. Genes Dev 2014 Oct 1 [PMID: 25233904] (ChIP, Human)
• Acharya S, Kaul Z, Gocha AS et al. Association of BLM and BRCA1 during Telomere Maintenance in ALT Cells. PLoS ONE. 2014 Aug 02 [PMID: 25084169] (WB, Human)
  
  Details:
  TRF2 antibody used for WB on experiments involving MG63 (osteosarcoma, ATCC), HeLa (cervical adenocarcinoma, ATCC), U2OS (osteosarcoma, ATCC) and Saos-2 (osteosarcoma, ATCC) cells. WB data images not shown in paper.
• Bai Y, Lathia JD, Zhang P et al. Molecular targeting of TRF2 suppresses the growth and tumorigenesis of glioblastoma stem cells. Glia. 2014 Jun 07 [PMID: 24909307] (WB, Human)
  
  Details:
  Figs 6, S1: Primary glioblastoma cells (GSCs) and GSCs lentivirus-infected with TRF2-shRNA. TRF2 was detected as a double at ~65 kDa. The specificity of the TRF2 mAb was validated in TRF2-shRNA lentivirus-infected GSCs by WB (Figs 6, S1).
• Edwards DN, Orren DK, Machwe A et al. Strand exchange of telomeric DNA catalyzed by the Werner syndrome protein (WRN) is specifically stimulated by TRF2. Nucleic Acids Res. 2014 Aug 01 [PMID: 24880691] (WB, Human)
  
  Details:
  Fig 7, TRF2 displacement assays using recombinant TRF2. The specificity of the TRF2 mAb was validated with TRF2 recombinant protein by WB in Fig 7.
• Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE. 2014 May 23 [PMID: 24854039] (WB, Mouse)
  
  Details:
  Liver, Fig 8.
• Ohishi T, Muramatsu Y, Yoshida H, Seimiya h. TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation. Mol. Cell. Biol. 2014 Apr 21 [PMID: 24752893] (ICC/IF, Human)
  
  Details:
  HeLa, The TRF2 mAb was used at 5 ug/ml.
• Stimpson KM, Sullivan LL, Kuo ME, Sullivan BA. Nucleolar organization, ribosomal DNA array stability, and acrocentric chromosome integrity are linked to telomere function. PLoS ONE 2014 Mar 25 [PMID: 24662969] (ICC/IF, Human)
  
  Details:
  HT1080 cells, Fig S1. the specificity of the TRF2 Ab was validated in TRF2-shRNA transfected HT1080 cells by IF (Fig S1).
• Popuri V, Hsu J, Khadka P et al. Human RECQL1 participates in telomere maintenance. Nucleic Acids Res. 2014 Mar 12 [PMID: 24623817] (WB, IP, Human)
  
  Details:
  U2OS cells, Figs 1A-D, S1E. The specificity of the TRF2 was validated with TRF2 siRNA in U2OS cells by IP/WB (Figs 1C, S1E). The TRF2 mAb co-IPapprox. d RECQL1, likewise a RECQL1 antibody co-IPapprox. d TRF2 which was recognized by the TRF2 mAb in WB.
• Munch Sandra, Weidtkamp-Peters Stefanie, Klement Karolin et al. The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence. Mol Cell Biol. 2014 May [PMID: 24615016]
• Queisser Angela, Heeg Steffen, Thaler Michaela et al. Inhibition of telomerase induces alternative lengthening of telomeres during human esophageal carcinogenesis. Cancer Genet. 2013 Nov [PMID: 24331919] (Human)
• Nakashima M, Nandakumar J, Sullivan KD et al. Inhibition of Telomerase Recruitment and Cancer Cell Death. J Biol Chem 2013 Nov 15 [PMID: 24097987] (ICC/IF, Human)
• Saha Bidisha, Zitnik Galynn, Johnson Simon et al. DNA damage accumulation and TRF2 degradation in atypical Werner syndrome fibroblasts with LMNA mutations. Front Genet. 2013 [PMID: 23847654] (WB)
• Kamranvar SA, Chen X, Masucci MG. Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus. Oncogene. 2013 May 27 [PMID: 23708666]
• Zhou J, Richardson M, Reddy V et al. Structural and functional association of androgen receptor with telomeres in prostate cancer cells. Aging (Albany NY). 2013 Jan [PMID: 23363843]
• Gocha AR, Nuovo G, Iwenofu OH, Groden J. Human sarcomas are mosaic for telomerase-dependent and telomerase-independent telomere maintenance mechanisms: implications for telomere-based therapies. Am J Pathol. 2013 Jan [PMID: 23260199]
• Nandakumar J, Bell CF, Weidenfeld I et al. The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity. Nature. 2012 Dec 13 [PMID: 23103865]
• Orlotti NI, Cimino-Reale G, Borghini E et al. Autophagy acts as a safeguard mechanism against G-quadruplex ligand-mediated DNA damage. Autophagy. 2012 Aug [PMID: 22627293]
• Suram A, Kaplunov J, Patel PL et al. Oncogene-induced telomere dysfunction enforces cellular senescence in human cancer precursor lesions. EMBO J. 2012 Jun 29 [PMID: 22569128]
• Galati A, Magdinier F, Colasanti V et al. TRF2 controls telomeric nucleosome organization in a cell cycle phase-dependent manner. PLoS One. 2012 [PMID: 22536324]
• Bhanot M, Smith S. TIN2 stability is regulated by the E3 ligase Siah2. Mol Cell Biol. 2012 Jan [PMID: 22064479]
• Ghosh AK, Rossi ML, Singh DK et al. RECQL4, the protein mutated in Rothmund-Thomson syndrome, functions in telomere maintenance. J Biol Chem. 2012 Jan 2 [PMID: 22039056]
• Abreu Eladio, Terns Rebecca M, Terns Michael P. Visualization of human telomerase localization by fluorescence microscopy techniques. Methods Mol Biol. 2011 [PMID: 21461817] (Human)
• Kamranvar SA, Masucci MG. The Epstein-Barr virus nuclear antigen-1 promotes telomere dysfunction via induction of oxidative stress. Leukemia. 2011 Jun [PMID: 21394098]
• Rodier F, Munoz DP, Teachenor R et al. DNA-SCARS: distinct nuclear structures that sustain damage-induced senescence growth arrest and inflammatory cytokine secretion. J Cell Sci. 2011 Jan 1 [PMID: 21118958]
• Stimpson Kaitlin M, Song Ihn Young, Jauch Anna et al. Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes. PLoS Genet. 2010 Aug [PMID: 20711355] (Human)
• Brugat Thibaut, Nguyen-Khac Florence, Grelier Aurore et al. Telomere dysfunction-induced foci arise with the onset of telomeric deletions and complex chromosomal aberrations in resistant chronic lymphocytic leukemia cells. Blood. 2010 Jul 15 [PMID: 20424183] (ICC/IF, Human)
• Abreu E, Aritonovska E, Reichenbach P et al. TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo. Mol Cell Biol. 2010 Jun [PMID: 20404094] (ICC/IF)
  
  Details:
  IF, HeLa and TPP1 depleted super telomerase HeLa cells, Fig 6.
• Yoshitake Kazutoshi, Aoyagi Hideyuki, Fujiwara Haruhiko. Creation of a novel telomere-cutting endonuclease based on the EN domain of telomere-specific non-long terminal repeat retrotransposon, TRAS1. Mob DNA. 2010 [PMID: 20359340] (WB)
• Tempera I, Deng Z, Atanasiu C et al. Regulation of Epstein-Barr virus OriP replication by poly(ADP-ribose) polymerase 1. J Virol. 2010 May [PMID: 20219917]
  
  Details:
  TRF2 (IMG-124A) and Tankyrase (IMG-146A) were both detected using western blot, Raji nuclear extracts, Fig 5A.
• Gocha AR, Acharya S, Groden J. WRN Loss Induces Switching of Telomerase-Independent Mechanisms of Telomere Elongation PLoS ONE (ICC/IF, Human)
  
  Details:
  This publication used the Alexa Fluor (R) 647 conjugated form of this antibody (Cat# NBP2-24823).
• Benson Erica K, Lee Sam W, Aaronson Stuart A. Role of progerin-induced telomere dysfunction in HGPS premature cellular senescence. J Cell Sci. 2010 Aug 1 [PMID: 20605919] (Human)
• Kim SH, Richardson M, Chinnakannu K et al. Androgen receptor interacts with telomeric proteins in prostate cancer cells. J Biol Chem. 2010 Apr 2 [PMID: 20110352] (WB,IF,IP)
  
  Details:
  Product cited: TRF2 (IMG-124A). 1. IF, Fig 1B: LNCaP cells treated with Casodex. 2. WB, Fig 2C: LNCaP cells treated with Casodex. 3. WB, Fig 4A: LNCaP, PPC-1, PC-3, and normal prostate epithelial cells. 4. IP, Fig 4B: LNCaP cells, detection of TRF2 follow
• Cesare AJ, Kaul Z, Cohen SB et al. Spontaneous occurrence of telomeric DNA damage response in the absence of chromosome fusions. Nat Struct Mol Biol. 2009 Dec [PMID: 19935685]
• Brouwer Anneke K, Schimmel Joost, Wiegant Joop C A G et al. Telomeric DNA mediates de novo PML body formation. Mol Biol Cell. 2009 Nov [PMID: 19793919]
• Draskovic Irena, Arnoult Nausica, Steiner Villier et al. Probing PML body function in ALT cells reveals spatiotemporal requirements for telomere recombination. Proc Natl Acad Sci U S A. 2009 Sep 15 [PMID: 19717459] (Human)
• Caslini C, Connelly JA, Serna A et al. MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription. Mol Cell Biol. 2009 Aug [PMID: 19528237] (WB, ChIP, IF)
  
  Details:
  Antibodies cited: TRF2 mAb (IMG-124a) and TRF2 pAb (IMG-148a) for ChIP, IF and WB. Cell llnes utilized: acute lymphoblastic leukemia (ALL) RS4;11 and ALL-PO cell lines, and the monoblastic, pre-B, and T-lineage leukemia cell lines U937, Nalm6, and Jurkat,
• Huda N, Tanaka H, Mendonca MS, Gilley D. DNA damage-induced phosphorylation of TRF2 is required for the fast pathway of DNA double-strand break repair. Mol Cell Biol. 2009 Jul [PMID: 19398584] (WB)
  
  Details:
  WB: TRF2 WT and TRF2 mutant (T188) transfected Saos and MCF7 cells (Figs 1 and 3). The TRF2 mAb recognized endogenous, WT and mutant (T188) TRF2.Note: The specificity of the antibody was validated with recombinantly expressed TRF2 by WB (Figs 1, 3).
• Bhattacharyya Saumitri, Keirsey Jeremy, Russell Beatriz et al. Telomerase-associated protein 1, HSP90, and topoisomerase IIalpha associate directly with the BLM helicase in immortalized cells using ALT and modulate its helicase activity using telomeric DNA substrates. J Biol Chem. 2009 May 29 [PMID: 19329795] (WB, Human)
• Tatsumi Y, Ezura K, Yoshida K et al. Involvement of human ORC and TRF2 in pre-replication complex assembly at telomeres. Genes Cells. 2008 Oct [PMID: 18761675] (WB)
  
  Details:
  HeLa cells were used with TRF2 IMG-124A (clone 4A794.15) mAb in the below figures. IF: Fig 3A WB: Figs 3E, 4A, 4B, 5A (shTRF2 retroviral infected HeLa), 5, 6A ChIP: Figs 3B, 3C, 3F, 5D, 5F, 5H, 6B, 6C. Note: The TRF2 antibody was shRNA validated in Fig 5A
• Huang FC, Chang CC, Lou PJ et al. G-quadruplex stabilizer 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide induces accelerated senescence and inhibits tumorigenic properties in cancer cells. Mol Cancer Res. 2008 Jun [PMID: 18515756] (ICC/IF, Human)
  
  Details:
  IF (human H1299 cancer cells), Fig. 5D.
• Kim Sahn-Ho, Davalos Albert R, Heo Seok-Jin et al. Telomere dysfunction and cell survival: roles for distinct TIN2-containing complexes. J Cell Biol. 2008 May 5 [PMID: 18443218] (IP)
• Muramatsu Y, Tahara H, Ono T et al. Telomere elongation by a mutant tankyrase 1 without TRF1 poly(ADP-ribosyl)ation. Exp Cell Res. 2008 Mar 10 [PMID: 18221737] (WB, Human)
  
  Details:
  WB; human HTC75 cells, Fig 4A.
• Zhang Y, Z Zhang, Z Miao, J Ding. Mol Pharmacol 73:824-832. 2008 [PMID: 18025071] (ChIP)
  
  Details:
  A459 human alveolar basal epithelial cancer cell line: IF [Figs 2A (top left) and, 5B] ChIP (Fig. 2C), WB [Figs. 2A (top right, bottom left, and right), 2B (bottom left), 3F (The TRF2 antibody was SiRNA validated using TRF2 SiRNA and mock-transfected A549
• Cristofari Gael, Adolf Emem, Reichenbach Patrick et al. Human telomerase RNA accumulation in Cajal bodies facilitates telomerase recruitment to telomeres and telomere elongation. Mol Cell. 2007 Sep 21 [PMID: 17889662] (ICC/IF, Human)
• Jiang WQ, Zhong ZH, Henson JD et al. Identification of candidate alternative lengthening of telomeres genes by methionine restriction and RNA interference. Oncogene. 2007 Jul 12 [PMID: 17297460]
• Barcaroli D, Dinsdale D, Neale M H et al. FLASH is an essential component of Cajal bodies. Proc Natl Acad Sci U S A. 2006 Oct 3 [PMID: 17003126]
• Barcaroli D, Bongiorno-Borbone L, Terrinoni A et al. FLASH is required for histone transcription and S-phase progression. Proc Natl Acad Sci U S A. 2006 Oct 3 [PMID: 17003125]
• Raz V, Carlotti F, Vermolen BJ et al. Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells. J Cell Sci. 2006 Oct 15 [PMID: 17003109]
• Bellon Marcia, Datta Abhik, Brown Megan et al. Increased expression of telomere length regulating factors TRF1, TRF2 and TIN2 in patients with adult T-cell leukemia. Int J Cancer. 2006 Nov 1 [PMID: 16786598]
• Biroccio A, Rizzo A, Elli R et al. TRF2 inhibition triggers apoptosis and reduces tumourigenicity of human melanoma cells. Eur J Cancer. 2006 Aug [PMID: 16750909] (WB)
  
  Details:
  WB: Fig 1A (M14 and JR5 human melanoma cells infected with TRF2, TRF2 mutant, and control retroviruses). The mutant TRF2 retrovirus lacked the N and C terminal domains of TRF2 and is referred to as TRF2deltaBdeltaC. The IMG-124A TRF2 mAb recognized endoge
• Luciani JJ, Depetris D, Usson Y et al. PML nuclear bodies are highly organised DNA-protein structures with a function in heterochromatin remodelling at the G2 phase. J Cell Sci. 2006 Jun 15 [PMID: 16735446]
• Freibaum BD, Counter CM. hSnm1B is a novel telomere-associated protein. J Biol Chem. 2006 Jun 2 [PMID: 16606622] (WB)
  
  Details:
  1. Immunofluorescence, TRF2 transiently transfected 293T cells, Fig 1. 2. WB, TRF2 transfected 293T cells were IP'ed with GFP antibody and results were western blotted, Fig 3A. Note: TRF2 was transfected validated with immunofluorescence (Fig 1) and WB (F
• Tahara H, Shin-Ya K, Seimiya H et al. G-Quadruplex stabilization by telomestatin induces TRF2 protein dissociation from telomeres and anaphase bridge formation accompanied by loss of the 3' telomeric overhang in cancer cells. Oncogene. 2006 Mar 23 [PMID: 16302000] (WB, ChIP, Human)
  
  Details:
  1. WB [MRC-5-hTERT (immortalized human fibroblasts), 90p (breast epithelial cells), HeLa, SiHa (human cervical carcinoma cell line), MCF-7], Fig 4a..2. ChIP (SiHa, MCF-7, HeLa, 90p), Fig 4b-d..3. IF (SiHa), Fig 5.
• Tanaka H, Mendonca MS, Bradshaw PS et al. DNA damage-induced phosphorylation of the human telomere-associated protein TRF2. Proc Natl Acad Sci U S A. 2005 Oct 25 [PMID: 16223874]
• Johnson JE, Varkonyi RJ, Schwalm J et al. Multiple mechanisms of telomere maintenance exist in liposarcomas. Clin Cancer Res. 2005 Aug 1 [PMID: 16061847] (ICC/IF)
• Nils Hartmanna Harry Scherthan. Experimental Cell Research 306 (1): 64-74. 2005 [PMID: 15878333] (ICC/IF, Muntjac (deer))
• Kaminker P, Plachot C, Kim SH et al. Higher-order nuclear organization in growth arrest of human mammary epithelial cells: a novel role for telomere-associated protein TIN2. J Cell Sci. 2005 Mar 15 [PMID: 15741234]
• Tarlochan Nijjar, Ekaterina Bassett, James Garbe, Yasuhiro Takenaka, Martha R Stampfer, David Gilley Paul Yaswen. Oncogene 24: 3369-3376. 2005 [PMID: 15735711] (ICC/IF)
  
  Details:
  Products cited: 1. TRF2 (IMG-124A): WB, numerous breast cancer cell lines, Fig 1. 2. RAP1 (IMG-272): WB, HMEC cells, Fig 2.
• Bradshaw PS, Stavropoulos DJ, Meyn MS. Human telomeric protein TRF2 associates with genomic double-strand breaks as an early response to DNA damage. Nat Genet. 2005 Feb [PMID: 15665826] (WB)
  
  Details:
  IF: Fig 1 (irradiated SV40-transformed human fibroblasts), Fig S1 (irradiated primary human fibroblasts), Fig 5a (irradiated glioblastoma cells) WB: Fig 6 (primary human fibroblasts virally overexpressing TRF2). The antibody recognized both endogenous TRF
• Seimiya H, Muramatsu Y, Ohishi T, Tsuruo T. Tankyrase 1 as a target for telomere-directed molecular cancer therapeutics. Cancer Cell. 2005 Jan [PMID: 15652747]
• Ahn B, Harrigan JA, Indig FE et al. Regulation of WRN helicase activity in human base excision repair. J Biol Chem. 2004 Dec 17 [PMID: 15385537] (ICC/IF)
  
  Details:
  Immunofluorescense, U-2 osteosarcoma cells, Fig 4.
• Houghtaling BR, Cuttonaro L, Chang W, Smith S. A dynamic molecular link between the telomere length regulator TRF1 and the chromosome end protector TRF2. Curr Biol. 2004 Sep 21 [PMID: 15380063] (IP, WB, Human)
• Kim SH, Beausejour C, Davalos AR et al. TIN2 mediates functions of TRF2 at human telomeres. J Biol Chem. 2004 Oct 15 [PMID: 15292264] (ICC/IF, WB, Human)
• Fotiadou P, Henegariu O, Sweasy JB. DNA polymerase beta interacts with TRF2 and induces telomere dysfunction in a murine mammary cell line. Cancer Res. 2004 Jun 1 [PMID: 15172990] (ICC/IF, WB, Mouse)
• Seimiya H, Muramatsu Y, Smith S, Tsuruo T. Functional subdomain in the ankyrin domain of tankyrase 1 required for poly(ADP-ribosyl)ation of TRF1 and telomere elongation. Mol Cell Biol. 2004 Mar [PMID: 14966275]
• Deng Z, Atanasiu C, Burg JS et al. Telomere repeat binding factors TRF1, TRF2, and hRAP1 modulate replication of Epstein-Barr virus OriP. J Virol. 2003 Nov [PMID: 14581536] (WB, Human)
  
  Details:
  Human Raji cells (WB, Fig 2), (ChIP, Figs 3B and 4B); The TRF2 antibody was siRNA validated by WB of of cell extracts derived from siRNA-transfected/vector-transfected cells (Fig 6B).
• Dimitrios J Stavropoulos, Paul S Bradshaw et al. Hum. Mol. Genet., 11: 3135-3144. 2002 [PMID: 12444098] (WB, Human)
• Opresko PL, von Kobbe C, Laine JP et al. Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases. J Biol Chem. 2002 Oct 25 [PMID: 12181313] (WB, ICC/IF)
  
  Details:
  1. WB, HeLa nuclear extracts, Fig 1D. 2. Immunofluoresence, U-2 OS nucleus, Fig 2A.
• Deng Z, Lezina L, Chen CJ et al. Telomeric proteins regulate episomal maintenance of Epstein-Barr virus origin of plasmid replication. Mol Cell. 2002 Mar [PMID: 11931758]
• Broch D Cook, Jasmin N Dynek, William Chang, Grigoriy Shostak, Susan Smith. Mol. Cell. Biol., 22: 332-342. 2002 [PMID: 11739745] (WB, ICC/IF, Human)
• Johnson FB, Marciniak RA, McVey M et al. The Saccharomyces cerevisiae WRN homolog Sgs1p participates in telomere maintenance in cells lacking telomerase. EMBO J. 2001 Feb 15 [PMID: 11179234] (ICC/IF, Human)
• Scherthan H, Jerratsch M, Li B et al. Mammalian meiotic telomeres: protein composition and redistribution in relation to nuclear pores. Mol Biol Cell. 2000 Dec [PMID: 11102517] (IHC-P, Human)
  
  Details:
  IHC (frozen), human testis, Fig 4A.
• Okabe J, Eguchi A, Masago A et al. TRF1 is a critical trans-acting factor required for de novo telomere formation in human cells. Hum Mol Genet. 2000 Nov 1 [PMID: 11063723] (WB)
  
  Details:
  WB: Fig 4B (human cell lines [HeLa, 293, Raji, Saos-2 and others), Fig 4C (human primary fibroblasts and PBMC). Endogenous TRF2 was detected as a single or doublet band with apparent sizes of 65 and 69 kDa. The differences in these bands were not characte

Review for TRF-2 Antibody (NB100-56506) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Fernanda gutierrez rodrigues WB Human 09/08/2017

Summary

• Application: Western Blot
• Sample Tested: 293T lysate
• Species: Human
• Lot: AB070705A-4

FAQs for TRF-2 Antibody (NB100-56506). (Showing 1 - 4 of 4 FAQs).

1. Are the TRF-2 antibodies validated in Simple Western?
   • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
2. Does TRF-2 antibodies comes in lyophilized form?
   • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
3. What the theoretical molecular weight for TRF-2 antibodies?
   • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.
4. What is the immunogen sequence of this TRF-2 antibody?
   • The whole length of Baculovirus expressed TRF-2 protein.

Bioinformatics

• Gene Symbol: TERF2
• Entrez:
  • 7014(Human)
  • 21750(Mouse)
  • 361403(Rat)
• Uniprot:
  • Q15554()