TRF-2 Antibody (4A794.15) - BSA Free

Images

 
Simple Western: TRF-2 Antibody (4A794.15) [NB100-56506] - Image shows a specific band for TRF2 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. ...read more
Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis in human Jurkat cell lysate at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used.
Immunohistochemistry-Paraffin: TRF-2 Antibody (4A794.15) [NB100-56506] - Transitional cell carcinoma, urinary bladder, stained with TRF2 antibody (4 ug/mL), peroxidase-conjugate and DAB chromogen. Note specific nuclear ...read more
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Localization of candidate RNAs at telomeres. Localization of SNORD17 at telomeres in human U-2 OS cells. Cells were fixed and ...read more
Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Intracellular flow cytometric analysis of TRF2 in 10^6 human Jurkat cells using 0.1 ug of NB100-56506. The shaded histogram represents cells alone, blue ...read more
Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Total protein from mouse 3T3 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Localization of candidate RNAs at telomeres. Localization of NEAT1 at telomeres in human U-2 OS cells. Cells were fixed and sequentially ...read more
Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - An intracellular stain was performed on A431 cells with TRF2 Antibody [4A794.15] NB100-56506 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Staining of TRF2-bound telomeres in human HeLa cells (Courtesy of Fotiadou, et al, 2004).
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ma, MarSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, CyTOF-ready
Clone
4A794.15
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

TRF-2 Antibody (4A794.15) - BSA Free Summary

Description
The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.
Immunogen
This TRF-2 Antibody (4A794.15) was developed against Baculovirus expressed whole length TRF2 protein, used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).
Marker
Telomeres marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TERF2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • CyTOF-ready
  • ELISA 2 ug/ml
  • Flow Cytometry 0.1 ug/10^6 cells
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2 ug/10^6 cells
  • Proximity Ligation Assay
  • Simple Western 1:50
  • Western Blot 2-4 ug/ml
Application Notes
TRF-2 may be detected as a single band or as a doublet in Western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. Observed molecular weights could vary depending on molecular weight standards used and gel conditions.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Proximity Ligation Assay reported in scientific literature (PMID 27366950). Use in immunoblotting reported in scientific literature (PMID: 23708666).
Theoretical MW
59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-56506 in the following applications:

Publications
Read Publications using
NB100-56506 in the following applications:

  • 9 publications
  • 35 publications
  • IF
    2 publications
  • 1 publication
  • IP
    5 publications
  • PLA
    2 publications
  • WB
    35 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TRF-2 Antibody (4A794.15) - BSA Free

  • 4A794 trf2
  • 4A794
  • 4A794.15 trf2
  • 4A794.15
  • anti-trf2 4A794
  • anti-trf2 4A794.15
  • clone 4A794
  • clone 4A794.15
  • Telomeric DNA-binding protein
  • telomeric repeat binding factor 2
  • Telomeric repeat binding protein 2
  • telomeric repeat-binding factor 2
  • TERF2
  • TRBF2
  • TRF2
  • TRF-2
  • TTAGGG repeat binding factor 2
  • TTAGGG repeat-binding factor 2

Background

Originally discovered as part of shelterin complex, telomeric repeat-binding factor 2 (TRF2, also called TERF2 or TRB2) is a ubiquitously expressed nuclear protein (55-60 kDa) involved in telomere homeostasis. TRF2 contains an N-terminal GAR domain, a central TRFH dimerization domain, and a C-terminal SAND/MYB-type DNA binding domain. Trf2 RNA has 10 exons and alternative splicing in rodents produces a truncated form, TRF2-S, which lacks the DNA binding domain and nuclear localization signal (NLS) (1).

Both TRF2 and TRF1 bind to telomeric double stranded 5'-TTAGGG-3' DNA repeats, then recruit RAP1, TIN2, TPP1, and POT1 for the assembly of the shelterin complex. The telomeric association of TRF2 is greatly increased in the S phase of the cell cycle (2). Loss of TRF2 leads to telomere shortening, the DNA damage response, chromosomal instability, and replicative senescence. Interestingly, the contribution of TRF2 to telomere shortening via a telomerase-independent mechanism has also been reported (3). In conjunction with the exonuclease, Apollo, TRF2 protects telomeres during replication and negatively regulates the accumulation of DNA topoisomerase (TOP1, TOP2A and TOP2B).

TRF2 has been implicated in cancer, shown to be a major oncogene in telomerase-deficient mice. A link to Werner syndrome, a premature aging disease caused by the loss of WRN, has been reported based on TRF2 recruitment of WRN for processing of telomeric DNA (4). TRF2 expression is increased during human embryonic stem cell differentiation and has been shown to interact with Repressor Element-1 Silencing Transcription Factor (REST), protecting it from proteasomal degradation (5).

References

1. Grammatikakis, I., Zhang, P., Mattson, M. P., & Gorospe, M. (2016). The long and the short of TRF2 in neurogenesis. Cell cycle (Georgetown, Tex.), 15(22), 3026-3032. PMID: 27565210

2. Li, F., Kim, H., Ji, Z., Zhang, T., Chen, B., Ge, Y., Hu, Y., Feng, X., Han, X., Xu, H., Zhang, Y., Yu, H., Liu, D., Ma, W., & Songyang, Z. (2018). The BUB3-BUB1 Complex Promotes Telomere DNA Replication. Molecular cell, 70(3), 395-407. PMID: 29727616

3. Ancelin, K., Brunori, M., Bauwens, S., Koering, C. E., Brun, C., Ricoul, M., Pommier, J. P., Sabatier, L., & Gilson, E. (2002). Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2. Molecular and cellular biology, 22(10), 3474-3487. PMID: 11971978

4. Machwe A, Xiao L, & Orren DK. (2004) TRF2 recruits the Werner syndrome (WRN) exonuclease for processing of telomeric DNA. Oncogene. 23(1):149-56. PMID: 14712220.

5. Diotti, R., & Loayza, D. (2011). Shelterin complex and associated factors at human telomeres. Nucleus (Austin, Tex.), 2(2), 119-135. PMID: 21738835

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRF-2 Antibody (NB100-56506)(107)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Marsupial, Muntjac (deer).

We have publications tested in 7 applications: ChIP, ICC/IF, IF, IHC-P, IP, PLA, WB.


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Showing Publications 1 - 10 of 107. Show All 107 Publications.
Publications using NB100-56506 Applications Species
Yu EY, Zahid SS, Aloe S Et al. Reciprocal impacts of telomerase activity and ADRN/MES differentiation state in neuroblastoma tumor biology Communications biology Nov 19 2021 [PMID: 34799676] (WB, Human) WB Human
Mohanty Bk, Karam Ja, Howley Bv Et Al. Heterogeneous nuclear ribonucleoprotein E1 binds polycytosine DNA and monitors genome integrity Life science alliance Sep 1 2021 [PMID: 34272328] (ChIP) ChIP
Chen YL, Sheu JJ, Sun CK et al. MicroRNA-214 modulates the senescence of vascular smooth muscle cells in carotid artery stenosis Mol. Med. May 14 2020 [PMID: 32410577] (WB, Rat) WB Rat
Episkopou H, Diman A, Claude E et al. TSPYL5 Depletion Induces Specific Death of ALT Cells through USP7-Dependent Proteasomal Degradation of POT1 Molecular Cell Jul 1 2019 [PMID: 31278054] (ICC/IF) ICC/IF
Kim J, Sun C, Tran AD et al. The macroH2A1.2 histone variant links ATRX loss to alternative telomere lengthening Nat. Struct. Mol. Biol. Mar 1 2019 [PMID: 30833786] (ChIP, ICC/IF, Human) ChIP, ICC/IF Human
Kong X, Cruz G M S et al. Biphasic recruitment of TRF2 to DNA damage sites promotes non-sister chromatid homologous recombination repair. J Cell Sci May 12 2018 [PMID: 30404833] (ICC/IF, Human) ICC/IF Human
Fujiwara C, Muramatsu Y, Nishii M et al. Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells. Sci Rep. Oct 4 2018 [PMID: 30287851] (WB, Human) WB Human
Bernal A, Molto-Abad M, Dominguez D, Tusell L. Acute telomere deprotection prevents ongoing BFB cycles and rampant instability in p16INK4a-deficient epithelial cells Oncotarget Jun 5 2018 [PMID: 29930757] (WB, Human) WB Human
Li M, Yang X, Lu X et al. APE1 deficiency promotes cellular senescence and premature aging features Nucleic Acids Res. May 10 2018 [PMID: 29750271] (ICC/IF, Human) ICC/IF Human
Panneer Selvam S, Roth BM, Nganga R et al. Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3 J. Biol. Chem. May 10 2018 [PMID: 29748384] (Human) Human
Show All 107 Publications.

Review for TRF-2 Antibody (NB100-56506) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56506:
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Western Blot TRF-2 NB100-56506
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reviewed by:
Verified Customer
Western Blot Human 09/08/2017
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Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman
LotAB070705A-4

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TRF-2 Antibody (NB100-56506). (Showing 1 - 4 of 4 FAQs).

  1. Are the TRF-2 antibodies validated in Simple Western?
    • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
  2. Does TRF-2 antibodies comes in lyophilized form?
    • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
  3. What the theoretical molecular weight for TRF-2 antibodies?
    • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.
  4. What is the immunogen sequence of this TRF-2 antibody?
    • The whole length of Baculovirus expressed TRF-2 protein.

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Blogs on TRF-2.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

TRF2: Telomere Maintenance and Protection
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an...  Read full blog post.

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Verified Customer
09/08/2017
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol TERF2
Entrez
Uniprot