Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis in human Jurkat cell lysate at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Staining of TRF2-bound telomeres in human HeLa cells (Courtesy of Fotiadou, et al, 2004).
Immunohistochemistry-Paraffin: TRF-2 Antibody (4A794.15) [NB100-56506] - Formalin-fixed, paraffin-embedded transitional cell carcinoma, urinary bladder, stained with TRF2 antibody (4 ug/ml), peroxidase-conjugate and DAB ...read more
Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis using the Alexa Fluor (R) 647 conjugate. Staining of TRF2 in 10^6 human Jurkat cells using 10 ul (0.1 ug). The blue histogram represents isotype control ...read more
Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Intracellular flow cytometric analysis of TRF2 in 10^6 human Jurkat cells using 0.1 ug of NB100-56506. The shaded histogram represents cells alone, blue ...read more
Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56506. Staining of TRF2 in 10^6 human Jurkat cells using 10 ul (0.1 ug) of this antibody. The blue ...read more
Simple Western: TRF-2 Antibody (4A794.15) [NB100-56506] - Simple Western lane view shows a specific band for TRF2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
1. TLR2 may be detected as a single band or as a doublet in western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. However, observed molecular weights could vary depending on molecular weight standards used and gel conditions. 2. The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. 3. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Refer to the published literature for additional information. 4. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain. In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-56506 in the following applications:
TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
TRF2: Telomere Maintenance and Protection TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an... Read full blog post.