TRF-2 Antibody (4A794.15) - BSA Free

Simple Western: TRF-2 Antibody (4A794.15) [NB100-56506] - Image shows a specific band for TRF2 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Analysis in human Jurkat cell lysate at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used.

Immunohistochemistry-Paraffin: TRF-2 Antibody (4A794.15) [NB100-56506] - Transitional cell carcinoma, urinary bladder, stained with TRF2 antibody (4 ug/mL), peroxidase-conjugate and DAB chromogen. Note specific nuclear staining. Tumor/normal adjacent tissue array slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Localization of candidate RNAs at telomeres. Localization of SNORD17 at telomeres in human U-2 OS cells. Cells were fixed and sequentially incubated with Abs against TRF-2 and AlexaFluor 488-conjugated anti-mouse IgG. Subsequently, the cells were hybridized with the RNA probes and subjected to fluorescence microscopy. Three different cells are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123387), licensed under a CC-BY license.

Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - Intracellular flow cytometric analysis of TRF2 in 10^6 human Jurkat cells using 0.1 ug of NB100-56506. The shaded histogram represents cells alone, blue represents isotype control and red represents NB100-56506, anti-TRF2.

Western Blot: TRF-2 Antibody (4A794.15) [NB100-56506] - Total protein from mouse 3T3 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TRF2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Localization of candidate RNAs at telomeres. Localization of NEAT1 at telomeres in human U-2 OS cells. Cells were fixed and sequentially incubated with Abs against TRF-2 and AlexaFluor 488-conjugated anti-mouse IgG. Subsequently, the cells were hybridized with the RNA probes and subjected to fluorescence microscopy. Three different cells are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123387), licensed under a CC-BY license.

Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - An intracellular stain was performed on A431 cells with TRF2 Antibody [4A794.15] NB100-56506 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Staining of TRF2-bound telomeres in human HeLa cells (Courtesy of Fotiadou, et al, 2004).

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TRF-2 (4A794.15) conjugated to FITC [NB100-56506F] at 5 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - BSA Free [NB100-56506] - ZBTB48‐dependent loss of MTFP1 phenocopies MTFP1 depletionFluorescence microscopy analysis of the structure & localization of the mitochondrial network in HeLa WT & ZBTB48 KO clones. Mitochondria are marked with the MitoTracker dye (red), & nuclei are counterstained with DAPI (blue). Scale bars represent 20 μm.The same analysis as in (A) for U2OS WT & ZBTB48 KO clones. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28500257), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TRF-2 Antibody (4A794.15) - BSA Free [NB100-56506] - TRF2 delta B delta M expression induces chromosome end-to-end fusions in all inducible cell lines(A) Immunoblots of MCF-10A, TO, SH-TO & SV-TO cell lines with & without DOX & HEK 293T. After 96 h of DOX treatment, the inducible cell lines expressed the truncated TRF2 delta B delta M protein (50 kDa); in contrast, uninduced cell lines, MCF-10A parental cell line & HEK 293T cells displayed only the endogen TRF2 protein (66 kDa). Lamin B1 was used as loading control. (B) After sustained expression of TRF2 delta B delta M for 96 h there was a significant increase in aberrant metaphases only in TO cells when compared to uninduced matched cells. (C) Nevertheless, the efficacy of the inducible system was validated by the statistical increase of cells with end-to-end fusions in all inducible cell lines. (D) Moreover, a high incidence of fusions per cell was found in all modified cell lines after sustained TRF2 depletion. Data was presented as mean + SEM. (E) Example of TO, SH-TO & SV-TO karyotypes after 96 h of TRF2 delta B delta M expression. Open arrows indicate clonal aberrations in the parental MCF-10A cell line. Insets in the karyotype show rearranged chromosomes stained with centromeric (green) & telomeric (red) PNA probes. Note the presence of telomere FISH signals at the fusion point of chromatid- or chromosome-type end-to-end fusions. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.25502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody (4A794.15) - BSA Free [NB100-56506] - ZBTB48 ZnF11 necessary to bind to telomeres. Sequence‐specific DNA pull‐downs w/ either telomeric (TTAGGG)/a control sequence (GTGAGT) for FLAG‐ZBTB48 WT, domain deletion constructs for different zinc finger & combinations of deletion constructs w/ ZnF10/11 point mutants. Domain structures indicated - right.Sequence‐specific DNA pull‐downs for FLAG‐ZBTB48 WT & ZnF11 point mutant for telomeric repeat sequences of different phyla (green) & their respective scrambled controls (blue).Protein expression analysis of ZBTB48 by WB for cell lines used. GAPDH serves as a loading control.IF stainings for exogenous FLAG‐ZBTB48 WT & point mutants for ZnF10 & ZnF11 in U2OS cells. The same analysis as in Fig 1E performed & average co‐localization frequencies shown (n = 24–37 cells).Co‐localization analysis of endogenous ZBTB48/exogenous FLAG‐ZBTB48 WT w/ TRF2 in HeLa cells by IF (IF) staining. Image illustrating co‐localization between ZBTB48/FLAG‐ZBTB48 WT (green) & TRF2 (red) as a marker for telomeres is shown w/ DAPI (blue) used as nuclear counterstain. Co‐localization events indicated by white arrows. Quantification of frequency of co‐localization events (right) done after 3D reconstruction of acquired z‐stacks (n = 30 cells).Co‐localization analysis of endogenous ZBTB48/exogenous FLAG‐ZBTB48 WT w/ TRF2 in HeLa 1.3 cells by IF (IF) staining analogous to (E) (n = 30 cells).Co‐localization analysis of endogenous ZBTB48/exogenous FLAG‐ZBTB48 WT w/ TRF2 in HT1080 super‐telomerase cells by IF (IF) staining analogous to (E) (n = 30 cells).Data information: (D–G) Scale bars = 5 μm. Error bars indicate standard deviations, & P‐values based on Student's t‐test.Source data available online for this figure. Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/28500257), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Ma, Mar
• Applications: WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IP, WB, ChIP, CyTOF-ready
• Clone: 4A794.15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRF-2 Antibody (4A794.15) - BSA Free Summary

• Description: The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.
• Immunogen: This TRF-2 Antibody (4A794.15) was developed against Baculovirus expressed whole length TRF2 protein, used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).
• Marker: Telomeres marker
• Specificity: The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TERF2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • CyTOF-ready
  • ELISA 2 ug/ml
  • Flow (Intracellular)
  • Flow Cytometry 0.1 ug/10^6 cells
  • Immunoblotting reported in scientific literature (PMID 23708666)
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 5 ug/ml
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 2 ug/10^6 cells
  • Proximity Ligation Assay reported in scientific literature (PMID 27366950)
  • Simple Western 1:50
  • Western Blot 2-4 ug/ml
• Application Notes: TRF-2 may be detected as a single band or as a doublet in Western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. Observed molecular weights could vary depending on molecular weight standards used and gel conditions.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 80 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 59.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for TRF-2 Antibody (4A794.15) - BSA Free

• 4A794 trf2
• 4A794
• 4A794.15 trf2
• 4A794.15
• anti-trf2 4A794
• anti-trf2 4A794.15
• clone 4A794
• clone 4A794.15
• Telomeric DNA-binding protein
• telomeric repeat binding factor 2
• Telomeric repeat binding protein 2
• telomeric repeat-binding factor 2
• TERF2
• TRBF2
• TRF2
• TRF-2
• TTAGGG repeat binding factor 2
• TTAGGG repeat-binding factor 2

Background

Originally discovered as part of shelterin complex, telomeric repeat-binding factor 2 (TRF2, also called TERF2 or TRB2) is a ubiquitously expressed nuclear protein (55-60 kDa) involved in telomere homeostasis. TRF2 contains an N-terminal GAR domain, a central TRFH dimerization domain, and a C-terminal SAND/MYB-type DNA binding domain. Trf2 RNA has 10 exons and alternative splicing in rodents produces a truncated form, TRF2-S, which lacks the DNA binding domain and nuclear localization signal (NLS) (1).

Both TRF2 and TRF1 bind to telomeric double stranded 5'-TTAGGG-3' DNA repeats, then recruit RAP1, TIN2, TPP1, and POT1 for the assembly of the shelterin complex. The telomeric association of TRF2 is greatly increased in the S phase of the cell cycle (2). Loss of TRF2 leads to telomere shortening, the DNA damage response, chromosomal instability, and replicative senescence. Interestingly, the contribution of TRF2 to telomere shortening via a telomerase-independent mechanism has also been reported (3). In conjunction with the exonuclease, Apollo, TRF2 protects telomeres during replication and negatively regulates the accumulation of DNA topoisomerase (TOP1, TOP2A and TOP2B).

TRF2 has been implicated in cancer, shown to be a major oncogene in telomerase-deficient mice. A link to Werner syndrome, a premature aging disease caused by the loss of WRN, has been reported based on TRF2 recruitment of WRN for processing of telomeric DNA (4). TRF2 expression is increased during human embryonic stem cell differentiation and has been shown to interact with Repressor Element-1 Silencing Transcription Factor (REST), protecting it from proteasomal degradation (5).

References

1. Grammatikakis, I., Zhang, P., Mattson, M. P., & Gorospe, M. (2016). The long and the short of TRF2 in neurogenesis. Cell cycle (Georgetown, Tex.), 15(22), 3026-3032. PMID: 27565210

2. Li, F., Kim, H., Ji, Z., Zhang, T., Chen, B., Ge, Y., Hu, Y., Feng, X., Han, X., Xu, H., Zhang, Y., Yu, H., Liu, D., Ma, W., & Songyang, Z. (2018). The BUB3-BUB1 Complex Promotes Telomere DNA Replication. Molecular cell, 70(3), 395-407. PMID: 29727616

3. Ancelin, K., Brunori, M., Bauwens, S., Koering, C. E., Brun, C., Ricoul, M., Pommier, J. P., Sabatier, L., & Gilson, E. (2002). Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2. Molecular and cellular biology, 22(10), 3474-3487. PMID: 11971978

4. Machwe A, Xiao L, & Orren DK. (2004) TRF2 recruits the Werner syndrome (WRN) exonuclease for processing of telomeric DNA. Oncogene. 23(1):149-56. PMID: 14712220.

5. Diotti, R., & Loayza, D. (2011). Shelterin complex and associated factors at human telomeres. Nucleus (Austin, Tex.), 2(2), 119-135. PMID: 21738835

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRF-2 Antibody (NB100-56506)(116)

Publications using NB100-56506

• Yajun Wang, Wei Zhu, Yumi Jang, Joshua A Sommers, Gong Yi, Chandrakala Puligilla, Deborah L Croteau, Yibin Yang, Mihoko Kai, Yie Liu The RNA-binding motif protein 14 regulates telomere integrity at the interface of TERRA and telomeric R-loops Nucleic Acids Research 2023-12-11 [PMID: 37930826]
• Hou J, Yun Y, Jeon B et al. Ginsenoside F1-Mediated Telomere Preservation Delays Cellular Senescence Int J Mol Sci 2023-09-19 [PMID: 37762556] (Western Blot)
• Zhang J, Zhang F, Porter KI et al. Telomere dysfunction in Tert knockout mice delays BrafV600E -induced melanoma development International journal of cancer 2023-09-20 [PMID: 37727982] (IHC-P, Mouse)
  
  Details:
  Dilution: 1:300
• Kliszczak M, Moralli D, Jankowska JD et al. Loss of FAM111B protease mutated in hereditary fibrosing poikiloderma negatively regulates telomere length Frontiers in cell and developmental biology 2023-06-05 [PMID: 37342232] (ICC/IF, Human)
• Jacome Burbano MS, Robin JD, Bauwens S et al. Non-canonical telomere protection role of FOXO3a of human skeletal muscle cells regulated by the TRF2-redox axis Communications biology 2023-05-25 [PMID: 37231173] (Western Blot)
  
  Details:
  Dilution:1:200
• Eguchi A, Torres-Bigio SI, Koleckar K, Birnbaum F TRF2 rescues pathogenic phenotypes in Duchenne muscular dystrophy cardiomyocytes derived from human iPSCs bioRxiv 2022-01-01 [PMID: 36719921] (WB)
• Dinami R, Pompili L, Petti E et al. MiR-182-3p targets TRF2 and impairs tumor growth of triple-negative breast cancer EMBO molecular medicine 2022-11-25 [PMID: 36426578] (IHC-P, Human)
• Mendez-Bermudez A, Lototska L, Pousse M et al. Selective pericentromeric heterochromatin dismantling caused by TP53 activation during senescence Nucleic acids research 2022-07-22 [PMID: 35819196] (ICC, Human)
• Zhang L, Geng X, Wang F et al. 53BP1 regulates heterochromatin through liquid phase separation Nature communications 2022-01-18 [PMID: 35042897] (Western Blot)
• Yu EY, Zahid SS, Aloe S Et al. Reciprocal impacts of telomerase activity and ADRN/MES differentiation state in neuroblastoma tumor biology Communications biology 2021-11-19 [PMID: 34799676] (WB, Human)
• Mohanty Bk, Karam Ja, Howley Bv Et Al. Heterogeneous nuclear ribonucleoprotein E1 binds polycytosine DNA and monitors genome integrity Life science alliance 2021-09-01 [PMID: 34272328] (Chemotaxis)
• Smith S Persistent telomere cohesion protects aged cells from premature senescence Nat Commun 2020-07-05 [PMID: 32620872]
• Chen YL, Sheu JJ, Sun CK et al. MicroRNA-214 modulates the senescence of vascular smooth muscle cells in carotid artery stenosis Mol. Med. 2020-05-14 [PMID: 32410577] (WB, Rat)
• Episkopou H, Diman A, Claude E et al. TSPYL5 Depletion Induces Specific Death of ALT Cells through USP7-Dependent Proteasomal Degradation of POT1 Molecular Cell 2019-07-01 [PMID: 31278054] (ICC/IF)
• Kim J, Sun C, Tran AD et al. The macroH2A1.2 histone variant links ATRX loss to alternative telomere lengthening Nat. Struct. Mol. Biol. 2019-03-01 [PMID: 30833786] (ICC/IF, Chemotaxis, Human)
• Kong X, Cruz G M S et al. Biphasic recruitment of TRF2 to DNA damage sites promotes non-sister chromatid homologous recombination repair. J Cell Sci 2018-05-12 [PMID: 30404833] (ICC/IF, Human)
• Fujiwara C, Muramatsu Y, Nishii M et al. Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells. Sci Rep. 2018-10-04 [PMID: 30287851] (WB, Human)
• Bernal A, Molto-Abad M, Dominguez D, Tusell L. Acute telomere deprotection prevents ongoing BFB cycles and rampant instability in p16INK4a-deficient epithelial cells Oncotarget 2018-06-05 [PMID: 29930757] (WB, Human)
• Li M, Yang X, Lu X et al. APE1 deficiency promotes cellular senescence and premature aging features Nucleic Acids Res. 2018-05-10 [PMID: 29750271] (ICC/IF, Human)
• Panneer Selvam S, Roth BM, Nganga R et al. Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3 J. Biol. Chem. 2018-05-10 [PMID: 29748384] (Human)
• Mendez-Bermudez A, Lototska L et al. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2. Mol Cell 2018-03-05 [PMID: 29727617] (ICC/IF, WB, Human)
• Athmane N Comparing methods for visualising genomic loci in live mammalian cells Anal Chem 2017-11-10 [PMID: 29120617]
• Jahn A, Rane G, Paszkowski-Rogacz M et al. ZBTB48 is both a vertebrate telomere-binding protein and a transcriptional activator. EMBO Rep. 2017-05-12 [PMID: 28500257] (ICC/IF, Human)
• Jeitany M, Bakhos-Douaihy D, Silvestre DC et al. Opposite effects of GCN5 and PCAF knockdowns on the alternative mechanism of telomere maintenance. Oncotarget 2017-04-18 [PMID: 28412741] (ICC/IF, PLA, Human)
• Fouquerel E, Lormand J, Bose A et al. Oxidative guanine base damage regulates human telomerase activity. Nat. Struct. Mol. Biol. 2016-12-01 [PMID: 27820808] (ICC/IF, Human)
• Bisht K, Smith EM, Tesmer VM et al. Structural and functional consequences of a disease mutation in the telomere protein TPP1 Proc Natl Acad Sci U S A. 2016-11-14 [PMID: 27807141] (Chemotaxis, Human)
• Konishi A, Izumi T, Shimizu S TRF2 Protein Interacts with Core Histones to Stabilize Chromosome Ends Journal of Biological Chemistry 2016-09-01 [PMID: 27514743] (WB, IP, ICC/IF, Mouse, Human)
• Picco V, Coste I, Giraud-Panis MJ et al. ERK1/2/MAPK pathway-dependent regulation of the telomeric factor TRF2 Oncotarget. 2016-07-19 [PMID: 27366950] (PLA, Human)
• Shukla S, Schmidt JC, Goldfarb KC et al. Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects. Nat Struct Mol Biol 2016-04-01 [PMID: 26950371]
• Kim YH, Kim KT, Lee SJ et al. Image-aided Suicide Gene Therapy Utilizing Multifunctional hTERT-targeting Adenovirus for Clinical Translation in Hepatocellular Carcinoma. Theranostics. 2016-01-06 [PMID: 26909111] (ICC/IF, Human)
• Hasegawa D, Okabe S, Okamoto K et al. G-quadruplex ligand-induced DNA damage response coupled with telomere dysfunction and replication stress in glioma stem cells Biochem. Biophys. Res. Commun. 2016-02-26 [PMID: 26845351] (ICC/IF, Human)
• Altieri P, Barisione C, Lazzarini E et al. Testosterone Antagonizes Doxorubicin-Induced Senescence of Cardiomyocytes. J Am Heart Assoc. 2016-01-09 [PMID: 26746999] (WB, Mouse, Rat)
  
  Details:
  TRF-2 antibody clone 4A794.15 was used for WB analysis in experiments involving C57BL/6 murine primary neonatal cardiomyocytes and rat embryonic cardiac cell line H9c2.
• Xi L, Schmidt JC, Zaug AJ et al. A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biol. 2015-11-10 [PMID: 26553065] (ICC/IF, Human)
• Saquilabon Cruz GM, Kong X, Silva BA et al. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites Nucleic Acids Res 2016-02-18 [PMID: 26424850] (ICC/IF, Marsupial)
  
  Details:
  Potorous tridactylis (Long-nosed Potoroo)
• Clynes D, Jelinska C, Xella B et al. Suppression of the alternative lengthening of telomere pathway by the chromatin remodelling factor ATRX Nat Commun. 2015-07-06 [PMID: 26143912] (ICC/IF, Human)
  
  Details:
  TRF2 antibody clone 4A794.15 (Imgenex IMG-124A) was used for ICC-IF in experiments involving U2OS(ATRX) cells and the immunostaining assay implicated fixation in 4% paraformaldehyde, prepermeabilization with ice cold 0.5% Triton X-100 for 5?min, use of primary antibody at 1:200 dilution, detection using Alexa conjugated secondary antibodies (Figure 1d, Figure 3, Figure 5, Supplementary Figure 1d). ICC-IF was performed on HeLa cells also which were exposed or not to Pyridostatin/PDS, a highly selective G4-interacting ligand (Supplementary Figure 4b).
• Burla R, Carcuro M, Raffa GD et al. AKTIP/Ft1, a New Shelterin-Interacting Factor Required for Telomere Maintenance PLoS Genet. 2015-06-01 [PMID: 26110528] (WB, Human)
• Kocak H. Functional Analysis of ACD Mutations in Dyskeratosis Congenita Thesis. 2014-01-01 (Chemotaxis, Human)
• Wiltshire T RECQL4; Linking DNA Replication and Bone Tumourigenesis Thesis
• Panneer Selvam S, De Palma RM, Oaks JJ et al. Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation Sci Signal. 2015-06-17 [PMID: 26082434]
• Fujita T, Yuno M, Okuzaki D et al. Identification of Non-Coding RNAs Associated with Telomeres Using a Combination of enChIP and RNA Sequencing PLoS ONE 2015-04-16 [PMID: 25874893] (ICC/IF, Human)
• Schertzer M, Jouravleva K, Perderiset M et al. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA Nucleic Acids Res. 2015-01-27 [PMID: 25628358] (ICC/IF, Human)
• Salvati E, Rizzo A, Iachettini S et al. A basal level of DNA damage and telomere deprotection increases the sensitivity of cancer cells to G-quadruplex interactive compounds Nucleic Acids Res. 2015-01-23 [PMID: 25618850] (WB, Chemotaxis, Human)
  
  Details:
  TRF2 antibody used for WB on lysates of BJ-hTERT fibroblasts cells expressing shGFP, shP53, shRb or BJ-EHLT (Figure 5G), and on BJ-hTERT fibroblasts transfected with siRNA-GFP and siRNA-TRF2 (Figure 6A). WB also performed on lysates of cell lines namely - BJ, BJ-hTERT, BJ-EHLT, BJ-EHLT/ Ras (Figure S5c). The antibody also used for ChIP assay on BJ-hTERT cells treated or not with 1 or 5 uM of RHPS4, G-quadruplex/G4 ligand for 24 hours (Figure 2F).
• Freund A, Zhong Fl, Venteicher As et al. Proteostatic Control of Telomerase Function through TRiC-Mediated Folding of TCAB1. Cell. [PMID: 25467444] (ICC/IF, Human)
  
  Details:
  Citation using the Azide Free form of this antibody.
• Kocak H, Ballew BJ, Bisht K et al. Hoyeraal-Hreidarsson syndrome caused by a germline mutation in the TEL patch of the telomere protein TPP1. Genes Dev. 2014-10-01 [PMID: 25233904] (Chemotaxis, Human)
• Acharya S, Kaul Z, Gocha AS et al. Association of BLM and BRCA1 during Telomere Maintenance in ALT Cells. PLoS ONE. 2014-08-02 [PMID: 25084169] (WB, Human)
  
  Details:
  TRF2 antibody used for WB on experiments involving MG63 (osteosarcoma, ATCC), HeLa (cervical adenocarcinoma, ATCC), U2OS (osteosarcoma, ATCC) and Saos-2 (osteosarcoma, ATCC) cells. WB data images not shown in paper.
• Bai Y, Lathia JD, Zhang P et al. Molecular targeting of TRF2 suppresses the growth and tumorigenesis of glioblastoma stem cells. Glia. 2014-06-07 [PMID: 24909307] (WB, Human)
  
  Details:
  Figs 6, S1: Primary glioblastoma cells (GSCs) and GSCs lentivirus-infected with TRF2-shRNA. TRF2 was detected as a double at ~65 kDa. The specificity of the TRF2 mAb was validated in TRF2-shRNA lentivirus-infected GSCs by WB (Figs 6, S1).
• Edwards DN, Orren DK, Machwe A et al. Strand exchange of telomeric DNA catalyzed by the Werner syndrome protein (WRN) is specifically stimulated by TRF2. Nucleic Acids Res. 2014-08-01 [PMID: 24880691] (WB, Human)
  
  Details:
  Fig 7, TRF2 displacement assays using recombinant TRF2. The specificity of the TRF2 mAb was validated with TRF2 recombinant protein by WB in Fig 7.
• Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS One 2014-05-24 [PMID: 24854039] (WB, Mouse)
• Ohishi T, Muramatsu Y, Yoshida H, Seimiya h. TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation. Mol. Cell. Biol. 2014-04-21 [PMID: 24752893] (ICC/IF, Human)
  
  Details:
  HeLa, The TRF2 mAb was used at 5 ug/ml.
• Stimpson K, Sullivan L, Kuo M et al. Nucleolar organization, ribosomal DNA array stability, and acrocentric chromosome integrity are linked to telomere function Plos One 2014-03-24 [PMID: 24662969] (ICC/IF, Human)
• Popuri V, Hsu J, Khadka P et al. Human RECQL1 participates in telomere maintenance. Nucleic Acids Res. 2014-03-12 [PMID: 24623817] (WB, IP, Human)
  
  Details:
  U2OS cells, Figs 1A-D, S1E. The specificity of the TRF2 was validated with TRF2 siRNA in U2OS cells by IP/WB (Figs 1C, S1E). The TRF2 mAb co-IPapprox. d RECQL1, likewise a RECQL1 antibody co-IPapprox. d TRF2 which was recognized by the TRF2 mAb in WB.
• Munch Sandra, Weidtkamp-Peters Stefanie, Klement Karolin et al. The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence. Mol Cell Biol. 2014-05-01 [PMID: 24615016]
• Queisser Angela, Heeg Steffen, Thaler Michaela et al. Inhibition of telomerase induces alternative lengthening of telomeres during human esophageal carcinogenesis. Cancer Genet. 2013-11-01 [PMID: 24331919] (Human)
• Nakashima M, Nandakumar J, Sullivan KD et al. Inhibition of Telomerase Recruitment and Cancer Cell Death. J Biol Chem 2013-11-15 [PMID: 24097987] (ICC/IF, Human)
• Saha Bidisha, Zitnik Galynn, Johnson Simon et al. DNA damage accumulation and TRF2 degradation in atypical Werner syndrome fibroblasts with LMNA mutations. Front Genet. 2013-01-01 [PMID: 23847654] (WB)
• Kamranvar SA, Chen X, Masucci MG. Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus. Oncogene. 2013-05-27 [PMID: 23708666]
• Kliszczak M, Moralli D, Jankowska J et al. Loss of FAM111B protease mutated in hereditary fibrosing poikiloderma syndrome negatively regulates telomere length bioRxiv 2023-01-23
• Zhou J, Richardson M, Reddy V et al. Structural and functional association of androgen receptor with telomeres in prostate cancer cells. Aging (Albany NY). 2013-01-01 [PMID: 23363843]
• Gocha AR, Nuovo G, Iwenofu OH et al. Human sarcomas are mosaic for telomerase-dependent and telomerase-independent telomere maintenance mechanisms: implications for telomere-based therapies. Am J Pathol. 2013-01-01 [PMID: 23260199]
• Nandakumar J, Bell CF, Weidenfeld I et al. The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity. Nature. 2012-12-13 [PMID: 23103865]
• Orlotti NI, Cimino-Reale G, Borghini E et al. Autophagy acts as a safeguard mechanism against G-quadruplex ligand-mediated DNA damage. Autophagy. 2012-08-01 [PMID: 22627293]
• Suram A, Kaplunov J, Patel PL et al. Oncogene-induced telomere dysfunction enforces cellular senescence in human cancer precursor lesions. EMBO J. 2012-06-29 [PMID: 22569128]
• Galati A, Magdinier F, Colasanti V et al. TRF2 controls telomeric nucleosome organization in a cell cycle phase-dependent manner. PLoS One. 2012-01-01 [PMID: 22536324]
• Bhanot M, Smith S. TIN2 stability is regulated by the E3 ligase Siah2. Mol Cell Biol. 2012-01-01 [PMID: 22064479]
• Ghosh AK, Rossi ML, Singh DK et al. RECQL4, the protein mutated in Rothmund-Thomson syndrome, functions in telomere maintenance. J Biol Chem. 2012-01-02 [PMID: 22039056]
• Abreu Eladio, Terns Rebecca M, Terns Michael P. Visualization of human telomerase localization by fluorescence microscopy techniques. Methods Mol Biol. 2011-01-01 [PMID: 21461817] (Human)
• Kamranvar SA, Masucci MG. The Epstein-Barr virus nuclear antigen-1 promotes telomere dysfunction via induction of oxidative stress. Leukemia. 2011-06-01 [PMID: 21394098]
  
  Details:
  WB: TRF2 (IMG-124), Tankyrase (IMG-146), POT1 (IMG-5343A), and Tin2 (IMG-282). EBNA1 positive or negative Ramos and BJAB cell lines, Fig 4A.
• Rodier F, Munoz DP, Teachenor R et al. DNA-SCARS: distinct nuclear structures that sustain damage-induced senescence growth arrest and inflammatory cytokine secretion. J Cell Sci. 2011-01-01 [PMID: 21118958]
• Stimpson Kaitlin M, Song Ihn Young, Jauch Anna et al. Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes. PLoS Genet. 2010-08-01 [PMID: 20711355] (Human)
• Benson Erica K, Lee Sam W, Aaronson Stuart A. Role of progerin-induced telomere dysfunction in HGPS premature cellular senescence. J Cell Sci. 2010-08-01 [PMID: 20605919] (Human)
• Brugat Thibaut, Nguyen-Khac Florence, Grelier Aurore et al. Telomere dysfunction-induced foci arise with the onset of telomeric deletions and complex chromosomal aberrations in resistant chronic lymphocytic leukemia cells. Blood. 2010-07-15 [PMID: 20424183] (ICC/IF, Human)
• Abreu E, Aritonovska E, Reichenbach P et al. TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo. Mol Cell Biol. 2010-06-01 [PMID: 20404094] (ICC/IF)
• Yoshitake Kazutoshi, Aoyagi Hideyuki, Fujiwara Haruhiko. Creation of a novel telomere-cutting endonuclease based on the EN domain of telomere-specific non-long terminal repeat retrotransposon, TRAS1. Mob DNA. 2010-01-01 [PMID: 20359340] (WB)
• Tempera I, Deng Z, Atanasiu C et al. Regulation of Epstein-Barr virus OriP replication by poly(ADP-ribose) polymerase 1. J Virol. 2010-05-01 [PMID: 20219917]
• Kim S, Richardson M, Chinnakannu K, et al. Androgen receptor interacts with telomeric proteins in prostate cancer cells Journal of Biological Chemistry 2010-04-02 [PMID: 20110352] (IP, WB, IF)
• Cesare AJ, Kaul Z, Cohen SB et al. Spontaneous occurrence of telomeric DNA damage response in the absence of chromosome fusions. Nat Struct Mol Biol. 2009-12-01 [PMID: 19935685]
• Brouwer Anneke K, Schimmel Joost, Wiegant Joop C A G et al. Telomeric DNA mediates de novo PML body formation. Mol Biol Cell. 2009-11-01 [PMID: 19793919]
• Draskovic I, Arnoult N, Steiner V et al. Probing PML body function in ALT cells reveals spatiotemporal requirements for telomere recombination. Proc Natl Acad Sci U S A. [PMID: 19717459] (Human)
• Caslini C, Connelly JA, Serna A et al. MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription. Mol Cell Biol. 2009-08-01 [PMID: 19528237] (Chemotaxis, IF, WB)
• Huda N, Tanaka H, Mendonca MS, Gilley D. DNA damage-induced phosphorylation of TRF2 is required for the fast pathway of DNA double-strand break repair. Mol Cell Biol. 2009-07-01 [PMID: 19398584] (WB)
• Bhattacharyya Saumitri, Keirsey Jeremy, Russell Beatriz et al. Telomerase-associated protein 1, HSP90, and topoisomerase IIalpha associate directly with the BLM helicase in immortalized cells using ALT and modulate its helicase activity using telomeric DNA substrates. J Biol Chem. 2009-05-29 [PMID: 19329795] (WB, Human)
• Tatsumi Y, Ezura K, Yoshida K et al. Involvement of human ORC and TRF2 in pre-replication complex assembly at telomeres. Genes Cells. 2008-10-01 [PMID: 18761675] (WB)
  
  Details:
  HeLa cells were used with TRF2 IMG-124A (clone 4A794.15) mAb in the below figures. IF: Fig 3A WB: Figs 3E, 4A, 4B, 5A (shTRF2 retroviral infected HeLa), 5, 6A ChIP: Figs 3B, 3C, 3F, 5D, 5F, 5H, 6B, 6C. Note: The TRF2 antibody was shRNA validated in Fig 5A
• Huang FC, Chang CC, Lou PJ et al. G-quadruplex stabilizer 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide induces accelerated senescence and inhibits tumorigenic properties in cancer cells. Mol Cancer Res. 2008-06-01 [PMID: 18515756] (ICC/IF, Human)
• Kim Sahn-Ho, Davalos Albert R, Heo Seok-Jin et al. Telomere dysfunction and cell survival: roles for distinct TIN2-containing complexes. J Cell Biol. 2008-05-05 [PMID: 18443218] (IP)
• Muramatsu Y, Tahara H, Ono T et al. Telomere elongation by a mutant tankyrase 1 without TRF1 poly(ADP-ribosyl)ation. Exp Cell Res. 2008-03-10 [PMID: 18221737] (WB, Human)
• Zhang YW, Zhang ZX, Miao ZH, Ding J. The telomeric protein TRF2 is critical for the protection of A549 cells from both telomere erosion and DNA double-strand breaks driven by salvicine. Mol Pharmacol. 2008-03-01 [PMID: 18025071] (Chemotaxis)
• Cristofari Gael, Adolf Emem, Reichenbach Patrick et al. Human telomerase RNA accumulation in Cajal bodies facilitates telomerase recruitment to telomeres and telomere elongation. Mol Cell. 2007-09-21 [PMID: 17889662] (ICC/IF, Human)
• Jiang WQ, Zhong ZH, Henson JD et al. Identification of candidate alternative lengthening of telomeres genes by methionine restriction and RNA interference. Oncogene. 2007-07-12 [PMID: 17297460]
  
  Details:
  Tin/Tin2 (clone 59B288.1, IMG-282) and TRF2 (clone 4A794.15, IMG-124A) in IIICF/c human Li-Fraumeni syndrome spontaneously immortalized fibroblast cells treated with TIN2, TRF2 or control siRNA: WB (Fig 4a), IF (Figs 4d and 5). Additionally, the TRF2 mAb was also used for IF in Figs 3C, 6D Notes: The specificity of the Tin2 and TRF2 mAbs were siRNA validated by WB in Fig 4a and by IF in Figs 4d and 5. For IF, cells cultured in chamber slides were fixed for 15 min in 2% parafomaldehyde at room temperature and then permeabilized with methanol/acetone (1:1) at 20 degrees C for 15 min. They were incubated with the Tin2 or TRF2 mAb overnight at 4 degrees and then with secondary antibodies for 40 min at room temperature. In some cases, DAPI was included with the secondary antibody to visualize DNA.
• Barcaroli D, Dinsdale D, Neale M H et al. FLASH is an essential component of Cajal bodies. Proc Natl Acad Sci U S A. 2006-10-03 [PMID: 17003126]
• Barcaroli D, Bongiorno-Borbone L, Terrinoni A et al. FLASH is required for histone transcription and S-phase progression. Proc Natl Acad Sci U S A. 2006-10-03 [PMID: 17003125]
• Raz V, Carlotti F, Vermolen BJ et al. Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells. J Cell Sci. 2006-10-15 [PMID: 17003109]
• Bellon Marcia, Datta Abhik, Brown Megan et al. Increased expression of telomere length regulating factors TRF1, TRF2 and TIN2 in patients with adult T-cell leukemia. Int J Cancer. 2006-11-01 [PMID: 16786598]
• Biroccio A, Rizzo A, Elli R et al. TRF2 inhibition triggers apoptosis and reduces tumourigenicity of human melanoma cells. Eur J Cancer. 2006-08-01 [PMID: 16750909] (WB)
• Luciani JJ, Depetris D, Usson Y et al. PML nuclear bodies are highly organised DNA-protein structures with a function in heterochromatin remodelling at the G2 phase. J Cell Sci. 2006-06-15 [PMID: 16735446]
• Freibaum BD, Counter CM. hSnm1B is a novel telomere-associated protein. J Biol Chem. 2006-06-02 [PMID: 16606622] (WB)
• Tahara H, Shin-Ya K, Seimiya H et al. G-Quadruplex stabilization by telomestatin induces TRF2 protein dissociation from telomeres and anaphase bridge formation accompanied by loss of the 3' telomeric overhang in cancer cells. Oncogene. 2006-03-23 [PMID: 16302000] (WB, Chemotaxis, Human)
  
  Details:
  1. WB [MRC-5-hTERT (immortalized human fibroblasts), 90p (breast epithelial cells), HeLa, SiHa (human cervical carcinoma cell line), MCF-7], Fig 4a..2. ChIP (SiHa, MCF-7, HeLa, 90p), Fig 4b-d..3. IF (SiHa), Fig 5.
• Tanaka H, Mendonca MS, Bradshaw PS et al. DNA damage-induced phosphorylation of the human telomere-associated protein TRF2. Proc Natl Acad Sci U S A. 2005-10-25 [PMID: 16223874]
• Johnson JE, Varkonyi RJ, Schwalm J et al. Multiple mechanisms of telomere maintenance exist in liposarcomas. Clin Cancer Res. 2005-08-01 [PMID: 16061847] (ICC/IF)
• Hartmann N, Scherthan H. Characterization of the telomere complex, TERF1 and TERF2 genes in muntjac species with fusion karyotypes. Exp Cell Res. 2005-05-15 [PMID: 15878333] (ICC/IF, Muntjac (deer))
• Kaminker P, Plachot C, Kim SH et al. Higher-order nuclear organization in growth arrest of human mammary epithelial cells: a novel role for telomere-associated protein TIN2. J Cell Sci. 2005-03-15 [PMID: 15741234]
• Nijjar Tarlochan, Bassett Ekaterina, Garbe James et al. Accumulation and altered localization of telomere-associated protein TRF2 in immortally transformed and tumor-derived human breast cells. Oncogene. 2005-05-05 [PMID: 15735711] (ICC/IF)
• Bradshaw PS, Stavropoulos DJ, Meyn MS. Human telomeric protein TRF2 associates with genomic double-strand breaks as an early response to DNA damage. Nat Genet. 2005-02-01 [PMID: 15665826] (WB)
• Seimiya H, Muramatsu Y, Ohishi T, Tsuruo T. Tankyrase 1 as a target for telomere-directed molecular cancer therapeutics. Cancer Cell 2005-01-01 [PMID: 15652747]
• Ahn B, Harrigan JA, Indig FE et al. Regulation of WRN helicase activity in human base excision repair. J Biol Chem. 2004-12-17 [PMID: 15385537] (ICC/IF)
• Houghtaling BR, Cuttonaro L, Chang W, Smith S. A dynamic molecular link between the telomere length regulator TRF1 and the chromosome end protector TRF2. Curr Biol. 2004-09-21 [PMID: 15380063] (WB, IP, Human)
• Kim SH, Beausejour C, Davalos AR et al. TIN2 mediates functions of TRF2 at human telomeres. J Biol Chem. 2004-10-15 [PMID: 15292264] (ICC/IF, WB, Human)
• Fotiadou P, Henegariu O, Sweasy JB. DNA polymerase beta interacts with TRF2 and induces telomere dysfunction in a murine mammary cell line. Cancer Res. 2004-06-01 [PMID: 15172990] (ICC/IF, WB, Mouse)
• Seimiya H, Muramatsu Y, Smith S, Tsuruo T. Functional subdomain in the ankyrin domain of tankyrase 1 required for poly(ADP-ribosyl)ation of TRF1 and telomere elongation. Mol Cell Biol. 2004-03-01 [PMID: 14966275]
• Deng Z, Atanasiu C, Burg JS et al. Telomere repeat binding factors TRF1, TRF2, and hRAP1 modulate replication of Epstein-Barr virus OriP. J Virol. 2003-11-01 [PMID: 14581536] (WB, Human)
• Stavropoulos DJ, Bradshaw PS, Li X et al. The Bloom syndrome helicase BLM interacts with TRF2 in ALT cells and promotes telomeric DNA synthesis. Hum Mol Genet. 2002-12-01 [PMID: 12444098] (WB, Human)
• Opresko PL, von Kobbe C, Laine JP et al. Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases. J Biol Chem. 2002-10-25 [PMID: 12181313] (WB, ICC/IF)
• Deng Z, Lezina L, Chen CJ et al. Telomeric proteins regulate episomal maintenance of Epstein-Barr virus origin of plasmid replication. Mol Cell. 2002-03-01 [PMID: 11931758]
• Cook BD, Dynek JN, Chang W et al. Role for the related poly(ADP-Ribose) polymerases tankyrase 1 and 2 at human telomeres. Mol Cell Biol. 2002-01-01 [PMID: 11739745] (WB, ICC/IF, Human)
• Johnson FB, Marciniak RA, McVey M et al. The Saccharomyces cerevisiae WRN homolog Sgs1p participates in telomere maintenance in cells lacking telomerase. EMBO J. 2001-02-15 [PMID: 11179234] (ICC/IF, Human)
• Scherthan H, Jerratsch M, Li B et al. Mammalian meiotic telomeres: protein composition and redistribution in relation to nuclear pores. Mol Biol Cell. 2000-12-01 [PMID: 11102517] (IHC-P, Human)
• Okabe J, Eguchi A, Masago A et al. TRF1 is a critical trans-acting factor required for de novo telomere formation in human cells. Hum Mol Genet. 2000-11-01 [PMID: 11063723] (WB)
  
  Details:
  WB: Fig 4B (human cell lines [HeLa, 293, Raji, Saos-2 and others), Fig 4C (human primary fibroblasts and PBMC). Endogenous TRF2 was detected as a single or doublet band with apparent sizes of 65 and 69 kDa. The differences in these bands were not characte

Review for TRF-2 Antibody (NB100-56506) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Fernanda gutierrez rodrigues WB Human 09/08/2017

Summary

• Application: Western Blot
• Sample Tested: 293T lysate
• Species: Human
• Lot: AB070705A-4

FAQs for TRF-2 Antibody (NB100-56506). (Showing 1 - 4 of 4 FAQs).

1. Are the TRF-2 antibodies validated in Simple Western?
   • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
2. Does TRF-2 antibodies comes in lyophilized form?
   • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
3. What the theoretical molecular weight for TRF-2 antibodies?
   • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.
4. What is the immunogen sequence of this TRF-2 antibody?
   • The whole length of Baculovirus expressed TRF-2 protein.

Bioinformatics

• Gene Symbol: TERF2
• Entrez:
  • 7014(Human)
  • 21750(Mouse)
  • 361403(Rat)
• Uniprot:
  • Q15554()