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TRF-1 Antibody (TRF1 3H11) - BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
TRF1 3H11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-14397AF350
Alexa Fluor 405
NB120-14397AF405
Alexa Fluor 488
NB120-14397AF488
Alexa Fluor 532
NB120-14397AF532
Alexa Fluor 594
NB120-14397AF594
Alexa Fluor 647
NB120-14397AF647
Alexa Fluor 700
NB120-14397AF700
Alexa Fluor 750
NB120-14397AF750
Biotin
NB120-14397B
DyLight 350
NB120-14397UV
DyLight 405
NB120-14397V
DyLight 488
NB120-14397G
DyLight 550
NB120-14397R
DyLight 594
NB120-14397DL594
DyLight 650
NB120-14397C
DyLight 680
NB120-14397FR
DyLight 755
NB120-14397IR
FITC
NB120-14397F
HRP
NB120-14397H
Janelia Fluor 549
NB120-14397JF549
Janelia Fluor 646
NB120-14397JF646
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NB120-14397-0.025mg
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TRF-1 Antibody (TRF1 3H11) - BSA Free Summary

Immunogen
Human TRF1
Localization
Nuclear
Marker
Telomere Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TERF1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Control
TRF-1 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Notes

Positive control(s): Hela cell line.

Alternate Names for TRF-1 Antibody (TRF1 3H11) - BSA Free

  • NIMA-interacting protein 2
  • PIN-2
  • PIN2hTRF1-AS
  • Telomeric protein Pin2/TRF1
  • telomeric repeat binding factor (NIMA-interacting) 1
  • telomeric repeat binding factor 1
  • telomeric repeat binding protein 1
  • telomeric repeat-binding factor 1
  • TERF1
  • TRBF1
  • TRF1
  • TRF-1
  • TRF1FLJ41416
  • TRFt-TRF1
  • TTAGGG repeat-binding factor 1

Background

The TRF1 gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. It is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-TRF-2 (4A794.15) conjugated to FITC [NB100-56506F] at 5 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: TRF-2 Antibody (4A794.15) [NB100-56506] - Localization of candidate RNAs at telomeres. Localization of SNORD17 at telomeres in human U-2 OS cells. Cells were fixed and sequentially incubated with Abs against TRF-2 and AlexaFluor 488-conjugated anti-mouse IgG. Subsequently, the cells were hybridized with the RNA probes and subjected to fluorescence microscopy. Three different cells are shown. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0123387), licensed under a CC-BY licence.
Flow Cytometry: TRF-2 Antibody (4A794.15) [NB100-56506] - An intracellular stain was performed on A431 cells with TRF2 Antibody [4A794.15] NB100-56506 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).
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NBP1-31133
Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP1-31133] - Paraffin-embedded hepatoma, using antibody at 1:500 dilution.
Western Blot: TBP like protein TLP Antibody [NBP1-31133] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:500.
Western Blot: TBP like protein TLP Antibody [NBP1-31133] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:2000.
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Holo-Transferrin
2914-HT
Holo-Transferrin
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NBP2-55709
Western Blot: TIN2 Antibody [NBP2-55709] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
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Thyrotropin
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NBP2-94348
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Immunocytochemistry/Immunofluorescence: Thyrotropin Releasing Hormone Antibody [NBP2-94348] - Analysis of U2OS cells using TRH Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: Thyrotropin Releasing Hormone Antibody [NBP2-94348] - Analysis of L929 cells using TRH Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
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IL-2 [Unconjugated]
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NB100-292
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Immunoprecipitation: TERF2IP Antibody [NB100-292] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-RAP1 antibody used for IP at 6 ug per reaction. RAP1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-RAP1 antibody. For blotting immunoprecipitated RAP1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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TERT Antibody (2C4)
NB100-317
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MAB1455
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Prolactin was detected in immersion fixed paraffin-embedded sections of human testis using Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of sperm cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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RAP1A Antibody
(1D4-1A3)
NBP2-22527
Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).
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Rabex5 Antibody
NBP1-49938
Western Blot: Rabex5 Antibody [NBP1-49938] - RABGEF1 is important for mitochondrial clearance. YFP-Parkin stably expressing WT and RABGEF1-mAID HCT116 cells pre-treated with IAA were treated with valinomycin for the indicated times. Total cell lysates were analyzed by immunoblotting. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/31326), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Rabex5 Antibody [NBP1-49938] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- RABGEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Rabex5 Antibody [NBP1-49938] - Detection of Human and Mouse RABGEF1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RABGEF1 was also immunoprecipitated by rabbit anti-RABGEF1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RABGEF1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
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Applications: IHC, IHC-P, IP, WB
C-Reactive
Protein/CRP [HRP]
DCRP00
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Applications: ELISA
IL-4 [Unconjugated]
6507-IL/CF
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL.
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Applications: BA

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Product General Protocols

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Control Lysate(s)

TRF-1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TERF1
Entrez