TRERF1 Antibody

Western Blot: TRERF1 Antibody [NB100-81655] - Detection of Human TReP-132/TRERF1 on HeLa whole cell lysate using NB100-81655. TReP-132/TRERF1 was also immunoprecipitated by rabbit anti-TReP-132/TRERF1 antibody NB100-81654.

Immunohistochemistry: TRERF1 Antibody [NB100-81655] - Detection of human TReP-132/TRERF1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-TReP-132/TRERF1 (NB100-81655). Detection: DAB

Immunohistochemistry: TRERF1 Antibody [NB100-81655] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- TReP-132/TRERF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TRERF1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1150 and 1200 of human transcriptional regulating protein 132 (transcriptional regulating factor 1) using the numbering given in entry NP_277037.1 (GeneID 55809).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRERF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000 to 1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: NB100-81655 can be used in (Co-IP application PMID: 21258344).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRERF1 Antibody

• BCAR2
• Breast cancer estrogen resistance 2RAPAHSA277276
• dJ139D8.5
• FLJ21198
• rapa-1
• rapa-2
• RP1-139D8.5
• transcriptional regulating factor 1
• transcriptional regulating protein 132
• transcriptional-regulating factor 1
• Transcriptional-regulating protein 132
• TREP132
• TReP-132
• Zinc finger protein rapa
• Zinc finger transcription factor TReP-132

Background

The transcriptional regulating protein of 132 kDa (TReP-132/TRERF1) was identified as a transcription factor that interacts with and activates the promoter of the CYP11A1 gene, a gene that encodes cytochrome P450 side chain cleavage (P450scc) and catalyzes the first step in steroidgenesis. TRep-132 can function as a nuclear receptor co-activator and has been shown to interact with Sp1 and the progesterone receptor (PR) to suppress growth via activation of the gene promoters of the cyclin-dependent kinase inhibitors, p21 and p27.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRERF1 Antibody (NB100-81655)(1)

Publication using NB100-81655

• Bantscheff M, Hopf C, Savitski MM et al. Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes. Nat Biotechnol. 2011-01-23 [PMID: 21258344]

Bioinformatics

• Gene Symbol: TRERF1
• Entrez:
  • 55809(Human)
• Uniprot:
  • Q96PN7(Human)