TRERF1 Antibody - BSA Free

Immunoprecipitation: TRERF1 Antibody [NB100-81654] - Detection of human TRERF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRERF1 antibody NB100-81654 used for IP at 3 ug per reaction. TRERF1 was also immunoprecipitated by rabbit anti-TRERF1 antibody NB100-81655. For blotting immunoprecipitated TRERF1, NB100-81655 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TRERF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRERF1 Antibody - BSA Free (NB100-81654) is a polyclonal antibody validated for use in IHC and IP. Anti-TRERF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human transcriptional regulating protein 132 (transcriptional regulating factor 1) using the numbering given in entry NP_277037.1 (GeneID 55809).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRERF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: This antibody can be used for Co-IP application (PMID: 21258344). It is not recommended for Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRERF1 Antibody - BSA Free

• BCAR2
• Breast cancer estrogen resistance 2RAPAHSA277276
• dJ139D8.5
• FLJ21198
• rapa-1
• rapa-2
• RP1-139D8.5
• transcriptional regulating factor 1
• transcriptional regulating protein 132
• transcriptional-regulating factor 1
• Transcriptional-regulating protein 132
• TREP132
• TReP-132
• Zinc finger protein rapa
• Zinc finger transcription factor TReP-132

Background

The transcriptional regulating protein of 132 kDa (TReP-132/TRERF1) was identified as a transcription factor that interacts with and activates the promoter of the CYP11A1 gene, a gene that encodes cytochrome P450 side chain cleavage (P450scc) and catalyzes the first step in steroidgenesis. TRep-132 can function as a nuclear receptor co-activator and has been shown to interact with Sp1 and the progesterone receptor (PR) to suppress growth via activation of the gene promoters of the cyclin-dependent kinase inhibitors, p21 and p27.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRERF1 Antibody (NB100-81654)(1)

Publication using NB100-81654

• Bantscheff M, Hopf C, Savitski MM et al. Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes. Nat Biotechnol. 2011-01-23 [PMID: 21258344]

Bioinformatics

• Gene Symbol: TRERF1
• Entrez:
  • 55809(Human)
• Uniprot:
  • Q96PN7(Human)