TREM1 Antibody [Unconjugated]

Western blot shows lysates of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TREM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TREM-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ELISA(Cap), Func
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TREM1 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1
  Met1-Arg200
  Accession # Q9NP99
• Specificity: Detects human TREM-1 in ELISAs and Western blots. In ELISAs, less than 0.2% cross-reactivity with recombinant mouse (rm) TREM‑1 and rmTREM‑2b is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TREM1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Agonist Activity
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human TREM-1 Antibody (Catalog # AF1278)
  ELISA Detection: Human TREM-1 Biotinylated Antibody (Catalog # BAF1278)
  Standard: Recombinant Human TREM-1 Fc Chimera (Catalog # 1278-TR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREM1 Antibody [Unconjugated]

• CD354 antigen
• CD354
• TREM1
• TREM-1
• TREM-1Triggering receptor expressed on monocytes 1
• triggering receptor expressed on myeloid cells 1
• triggering-receptor TREM1

Background

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha , suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with
LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

1. Bouchon, A. (2000) J. Immunol. 164:4991.
2. Bouchon, A. (2001) Nature 410:1103.
3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TREM1 Antibody (AF1278)(8)

Publications using AF1278

• Christophe Boudesco, Annelies Nonneman, Alessandro Cinti, Paola Picardi, Loredana Redaelli, Sofie Swijsen et al. Novel potent liposome agonists of triggering receptor expressed on myeloid cells 2 phenocopy antibody treatment in cells Glia 2022-12-01 [PMID: 35912412]
• X Liu, W Chen, G Zhu, H Yang, W Li, M Luo, C Shu, Z Zhou Single-cell RNA sequencing identifies an Il1rn+/Trem1+ macrophage subpopulation as a cellular target for mitigating the progression of thoracic aortic aneurysm and dissection Cell Discovery, 2022-02-08;8(1):11. 2022-02-08 [PMID: 35132073] (IHC, Mouse)
• Ashok K. Pullikuth, Eric D. Routh, Kip D. Zimmerman, Julia Chifman, Jeff W. Chou, Michael H. Soike et al. Bulk and Single-Cell Profiling of Breast Tumors Identifies TREM-1 as a Dominant Immune Suppressive Marker Associated With Poor Outcomes Frontiers in Oncology 2021-12-08 [PMID: 34956864]
• Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm Journal of Clinical Investigation 2021-01-19 [PMID: 33258804] (Immunohistochemistry, Human)
• Shenzong Rao, Jingsong Huang, Zhijun Shen, Changgang Xiang, Min Zhang, Xueliang Lu Inhibition of TREM‐1 attenuates inflammation and lipid accumulation in diet‐induced nonalcoholic fatty liver disease Journal of Cellular Biochemistry 2019-07-01 [PMID: 30805986]
• Sankar Baruah, Kathy Keck, Michelle Vrenios, Marshall R. Pope, Merideth Pearl, Kevin Doerschug et al. Identification of a novel splice variant isoform of TREM-1 in human neutrophil granules1 The Journal of Immunology 2015-12-15 [PMID: 26561551]
• Amir Boufenzer, Jérémie Lemarié, Tabassome Simon, Marc Derive, Youcef Bouazza, Nguyen Tran et al. TREM-1 Mediates Inflammatory Injury and Cardiac Remodeling Following Myocardial Infarction Circulation Research 2015-05-22 [PMID: 25840803]
• Gomez-Pina V, Soares-Schanoski A, Rodriguez-Rojas A, Del Fresno C, Garcia F, Vallejo-Cremades MT, Fernandez-Ruiz I, Arnalich F, Fuentes-Prior P, Lopez-Collazo E Metalloproteinases shed TREM-1 ectodomain from lipopolysaccharide-stimulated human monocytes. J. Immunol., 2007-09-15;179(6):4065-73. 2007-09-15 [PMID: 17785845] (Flow Cytometry, ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: TREM1
• Uniprot:
  • Q9NP99()