TREM1 Antibody [Unconjugated]

TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS (left panel) or untreated (right panel) using Goat Anti-Mouse TREM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ELISA(Cap), Func, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TREM1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
  Ala21-Ser202
  Accession # Q9JKE2
• Specificity: Detects mouse TREM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human (rh) TREM-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TREM1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Agonist Activity
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse TREM-1 Antibody (Catalog # AF1187) ELISA Detection: Mouse TREM-1 Biotinylated Antibody (Catalog # BAF1187)
  Standard: Recombinant Mouse TREM-1 Fc Chimera (Catalog # 1187-TR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREM1 Antibody [Unconjugated]

• CD354 antigen
• CD354
• TREM1
• TREM-1
• TREM-1Triggering receptor expressed on monocytes 1
• triggering receptor expressed on myeloid cells 1
• triggering-receptor TREM1

Background

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

1. Bouchon, A. (2000) J. Immunol. 164:4991.
2. Bouchon, A. (2001) Nature 410:1103.
3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TREM1 Antibody (AF1187)(9)

Publications using AF1187

• Wu, K;Liu, YY;Shao, S;Song, W;Chen, XH;Dong, YT;Zhang, YM; The microglial innate immune receptors TREM-1 and TREM-2 in the anterior cingulate cortex (ACC) drive visceral hypersensitivity and depressive-like behaviors following DSS-induced colitis Brain, behavior, and immunity 2023-06-05 [PMID: 37286175] (Western Blot, Mouse)
• Dong Hyuk Seo, Xiumei Che, Soochan Kim, Da Hye Kim, Hyun Woo Ma, Jae Hyeon Kim et al. Triggering Receptor Expressed on Myeloid Cells-1 Agonist Regulates Intestinal Inflammation via Cd177+ Neutrophils Frontiers in Immunology 3/9/2021 [PMID: 33767714]
• Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm Journal of Clinical Investigation 1/19/2021 [PMID: 33258804]
• Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm Journal of Clinical Investigation 2021-01-19 [PMID: 33258804] (Immunohistochemistry, Mouse)
• K Schlepckow, KM Monroe, G Kleinberge, L Cantuti-Ca, S Parhizkar, D Xia, M Willem, G Werner, N Pettkus, B Brunner, A Sülzen, B Nuscher, H Hampel, X Xiang, R Feederle, S Tahirovic, JI Park, R Prorok, C Mahon, CC Liang, J Shi, DJ Kim, H Sabelström, F Huang, G Di Paolo, M Simons, JW Lewcock, C Haass Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region EMBO Mol Med, 2020-03-10;0(0):e11227. 3/10/2020 [PMID: 32154671]
• NL Denning, M Aziz, A Murao, SD Gurien, M Ochani, JM Prince, P Wang Extracellular CIRP as an endogenous TREM-1 ligand to fuel inflammation in sepsis JCI Insight, 2020-03-12;0(0):. 3/12/2020 [PMID: 32027618]
• Zhihong Yuan, Mansoor Syed, Dipti Panchal, Myungsoo Joo, Chetna Bedi, Sokbee Lim et al. TREM-1-accentuated lung injury via miR-155 is inhibited by LP17 nanomedicine American Journal of Physiology-Lung Cellular and Molecular Physiology 3/1/2016 [PMID: 26684249]
• Wu M, Peng A, Sun M, Deng Q, Hazlett L, Yuan J, Liu X, Gao Q, Feng L, He J, Zhang P, Huang X TREM-1 amplifies corneal inflammation after Pseudomonas aeruginosa infection by modulating Toll-like receptor signaling and Th1/Th2-type immune responses. Infect Immun, 2011-05-09;79(7):2709-16. 2011-05-09 [PMID: 21555403] (Bioassay, Western Blot, Mouse)
• Schenk M, Bouchon A, Seibold F, Mueller C TREM-1--expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases. J. Clin. Invest., 2007-10-01;117(10):3097-106. 2007-10-01 [PMID: 17853946] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: TREM1
• Uniprot:
  • Q9JKE2()