TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free

Flow Cytometry: TREM-1 Antibody (L5-B8.2A12.3A12) [NBP1-40907] - Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor 647

Flow Cytometry: TREM-1 Antibody (L5-B8.2A12.3A12) [NBP1-40907] - Staining of J774 cells with Rat anti Mouse TREM-1:RPE.

Flow Cytometry: TREM-1 Antibody (L5-B8.2A12.3A12) [NBP1-40907] - Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor 488

• Reactivity: Mu
• Applications: WB, Flow, Func, IHC, CyTOF-ready
• Clone: L5-B8.2A12.3A12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free (NBP1-40907) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Mouse TREM-1 Fc fusion protein
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TREM1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:25-1:100
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Funcional: TREM-1 mediates the septic shock pathway, and blocking TREM-1 has been reported to reduce inflammation and increase survival in certain models of bacterial infection. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Endotoxin Level <0.01EU/ug. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TREM1 Antibody (L5-B8.2A12.3A12) - Azide and BSA Free

• CD354 antigen
• CD354
• TREM1
• TREM-1
• TREM-1Triggering receptor expressed on monocytes 1
• triggering receptor expressed on myeloid cells 1
• triggering-receptor TREM1

Background

Monocyte/macrophage- and neutrophil-mediated inflammatory responses can be stimulated through a variety of receptors, including G protein-linked 7-transmembrane receptors (e.g., FPR1; MIM 136537), Fc receptors (see MIM 146790), CD14 (MIM 158120) and Toll-like receptors (e.g., TLR4; MIM 603030), and cytokine receptors (e.g., IFNGR1; MIM 107470). Engagement of these receptors can also prime myeloid cells to respond to other stimuli. Myeloid cells express receptors belonging to the Ig superfamily, such as TREM1, or to the C-type lectin superfamily. Depending on their transmembrane and cytoplasmic sequence structure, these receptors have either activating (e.g., KIR2DS1; MIM 604952) or inhibitory functions (e.g., KIR2DL1; MIM 604936).[supplied by OMIM] Mouse triggering receptor expressed on myeloid cells 1 (TREM-1), a 30kD member of the immunoglobulin superfamily expressed on neutrophils and a subset of monocytes. TREM-1 is involved in the microbial inflammatory response. It acts in conjunction with the adaptor protein DAP12 to activate neutrophils and monocytes, leading to the secretion of pro-inflammatory mediators and enhancement of the inflammatory response. Microbial infection also induces the release of soluble form of TREM-1 from monocytes, which is reported to act as an inhibitor of TREM-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TREM1
• Entrez:
  • 58217(Mouse)
• Uniprot:
  • Q9JKE2()
  • Q9JKE2()