TREM1 Antibody (193015) [Alexa Fluor® 488]

Human peripheral blood granulocytes were stained with Mouse Anti-Human TREM-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1278G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1278G, filled histogram) or isotype control antibody (IC002G). Staining was performed using our Staining Membrane-associated Proteins protocol.

TREM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1278G, filled histogram) or isotype control antibody (IC002G,open histogram). No staining in the TREM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow, KO
• Clone: 193015
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

TREM1 Antibody (193015) [Alexa Fluor® 488] Summary

• Additional Information: Clone 193015 was used by HLDA to establish CD designation
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1
  Ala21-Asn205
  Accession # Q9NP99
• Specificity: Detects human TREM-1 in ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TREM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells
  • Knockout Validated

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREM1 Antibody (193015) [Alexa Fluor® 488]

• CD354 antigen
• CD354
• TREM1
• TREM-1
• TREM-1Triggering receptor expressed on monocytes 1
• triggering receptor expressed on myeloid cells 1
• triggering-receptor TREM1

Background

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha , suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

1. Bouchon, A. (2000) J. Immunol. 164:4991.
2. Bouchon, A. (2001) Nature 410:1103.
3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TREM1
• Uniprot:
  • Q9NP99()