TREM1 Antibody (174031) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM‑1 Monoclonal Antibody (Catalog # MAB1187) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse TREM-1 Monoclonal Antibody (Catalog # MAB1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, Func, ICC/IF
• Clone: 174031
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

TREM1 Antibody (174031) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
  Ala21-Ser202
  Accession # Q9JKE2
• Specificity: Detects mouse TREM-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse TREM-2B, recombinant human (rh) TREM-1, or rhTREM-2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TREM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Agonist Activity
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREM1 Antibody (174031) [Unconjugated]

• CD354 antigen
• CD354
• TREM1
• TREM-1
• TREM-1Triggering receptor expressed on monocytes 1
• triggering receptor expressed on myeloid cells 1
• triggering-receptor TREM1

Background

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

1. Bouchon, A. (2000) J. Immunol. 164:4991.
2. Bouchon, A. (2001) Nature 410:1103.
3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TREM1 Antibody (MAB1187)(16)

Publications using MAB1187

• Li, Q;Zhao, Y;Guo, H;Li, Q;Yan, C;Li, Y;He, S;Wang, N;Wang, Q; Impaired lipophagy induced-microglial lipid droplets accumulation contributes to the buildup of TREM1 in diabetes-associated cognitive impairment Autophagy 2023-05-19 [PMID: 37204119] (IHC, Mouse)
• WJ Zhong, J Zhang, JX Duan, CY Zhang, SC Ma, YS Li, NS Yang, HH Yang, JB Xiong, CX Guan, ZX Jiang, ZJ You, Y Zhou TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury Journal of Translational Medicine, 2023-03-06;21(1):179. 2023-03-06 [PMID: 36879273] (ELISA Capture, Mouse)
• Zhong WJ, Liu T, Yang HH et al. TREM-1 governs NLRP3 inflammasome activation of macrophages by firing up glycolysis in acute lung injury International journal of biological sciences 1/1/2023 [PMID: 36594089]
  
  Details:
  Dilution used in ICC 1:100
• Sawaya AP, Stone RC, Mehdizadeh S et al. FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers EMBO reports 8/3/2022 [PMID: 35856334]
• Dong Hyuk Seo, Xiumei Che, Soochan Kim, Da Hye Kim, Hyun Woo Ma, Jae Hyeon Kim et al. Triggering Receptor Expressed on Myeloid Cells-1 Agonist Regulates Intestinal Inflammation via Cd177+ Neutrophils Frontiers in Immunology 3/9/2021 [PMID: 33767714]
• Deng Q, Yang S, Sun L, et al. Salmonella effector SpvB aggravates dysregulation of systemic iron metabolism via modulating the hepcidin-ferroportin axis Gut microbes 1/21/2021 [PMID: 33475464]
• Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm Journal of Clinical Investigation 1/19/2021 [PMID: 33258804]
• Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm Journal of Clinical Investigation 2021-01-19 [PMID: 33258804] (In vivo assay, Mouse)
• N Mukhamedov, A Hoang, D Dragoljevi, L Dubrovsky, T Pushkarsky, H Low, M Ditiatkovs, Y Fu, R Ohkawa, PJ Meikle, A Horvath, B Brichacek, YI Miller, A Murphy, M Bukrinsky, D Sviridov Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells PLoS Pathog., 2019-07-25;15(7):e1007907. 2019-07-25 [PMID: 31344124] (ICC, Mouse)
• Yong Sun Lee, In Jun Yeo, Ki Cheon Kim, Sang-Bae Han, Jin Tae Hong Inhibition of Lung Tumor Development in ApoE Knockout Mice via Enhancement of TREM-1 Dependent NK Cell Cytotoxicity Frontiers in Immunology 6/18/2019 [PMID: 31275318]
• S Baruah, S Murthy, K Keck, I Galvan, A Prichard, LH Allen, M Farrelly, J Klesney-Ta TREM-1 regulates neutrophil chemotaxis by promoting NOX-dependent superoxide production J. Leukoc. Biol., 2019-01-22;0(0):. 2019-01-22 [PMID: 30667543] (Flow Cytometry, Mouse)
• Z Yuan, X Fan, B Staitieh, C Bedi, P Spearman, DM Guidot, RT Sadikot HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1 Sci Rep, 2017-02-09;7(0):42028. 2017-02-09 [PMID: 28181540]
• Wu K, Byers D, Jin X, Agapov E, Alexander-Brett J, Patel A, Cella M, Gilfilan S, Colonna M, Kober D, Brett T, Holtzman M TREM-2 promotes macrophage survival and lung disease after respiratory viral infection. J Exp Med, 2015-04-20;212(5):681-97. 2015-04-20 [PMID: 25897174] (Flow Cytometry, Mouse)
• Weber B, Schuster S, Zysset D, Rihs S, Dickgreber N, Schurch C, Riether C, Siegrist M, Schneider C, Pawelski H, Gurzeler U, Ziltener P, Genitsch V, Tacchini-Cottier F, Ochsenbein A, Hofstetter W, Kopf M, Kaufmann T, Oxenius A, Reith W, Saurer L, Mueller C TREM-1 deficiency can attenuate disease severity without affecting pathogen clearance. PLoS Pathog, 2014-01-16;10(1):e1003900. 2014-01-16 [PMID: 24453980] (Functional Assay, Mouse)
• Ingersoll MA, Spanbroek R, Lottaz C Comparison of gene expression profiles between human and mouse monocyte subsets. Blood, 2009-11-12;115(3):e10-9. 2009-11-12 [PMID: 19965649] (Flow Cytometry, Mouse)
• Lagler H, Sharif O, Haslinger I, Matt U, Stich K, Furtner T, Doninger B, Schmid K, Gattringer R, de Vos AF, Knapp S TREM-1 activation alters the dynamics of pulmonary IRAK-M expression in vivo and improves host defense during pneumococcal pneumonia. J. Immunol., 2009-07-13;183(3):2027-36. 2009-07-13 [PMID: 19596984] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: TREM1
• Uniprot:
  • Q9JKE2()