TREM-1 Antibody (L5-B8.2A12.3A12)

Images

 
Flow Cytometry: TREM-1 Antibody (L5-B8.2A12.3A12) [NBP1-40907] - Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor 647
Flow Cytometry: TREM-1 Antibody (L5-B8.2A12.3A12) [NBP1-40907] - Staining of J774 cells with Rat anti Mouse TREM-1:RPE.
Flow Cytometry: TREM-1 Antibody (L5-B8.2A12.3A12) [NBP1-40907] - Staining of J774 cells with Rat anti Mouse TREM-1:Alexa Fluor 488

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC, IHC-Fr
Clone
L5-B8.2A12.3A12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TREM-1 Antibody (L5-B8.2A12.3A12) Summary

Immunogen
Mouse TREM-1 Fc fusion protein.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TREM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:25-1:100
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Funcional: TREM-1 mediates the septic shock pathway, and blocking TREM-1 has been reported to reduce inflammation and increase survival in certain models of bacterial infection. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Endotoxin Level <0.01EU/ug. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TREM-1 Antibody (L5-B8.2A12.3A12)

  • CD354 antigen
  • CD354
  • TREM1
  • TREM-1
  • TREM-1Triggering receptor expressed on monocytes 1
  • triggering receptor expressed on myeloid cells 1
  • triggering-receptor TREM1

Background

Monocyte/macrophage- and neutrophil-mediated inflammatory responses can be stimulated through a variety of receptors, including G protein-linked 7-transmembrane receptors (e.g., FPR1; MIM 136537), Fc receptors (see MIM 146790), CD14 (MIM 158120) and Toll-like receptors (e.g., TLR4; MIM 603030), and cytokine receptors (e.g., IFNGR1; MIM 107470). Engagement of these receptors can also prime myeloid cells to respond to other stimuli. Myeloid cells express receptors belonging to the Ig superfamily, such as TREM1, or to the C-type lectin superfamily. Depending on their transmembrane and cytoplasmic sequence structure, these receptors have either activating (e.g., KIR2DS1; MIM 604952) or inhibitory functions (e.g., KIR2DL1; MIM 604936).[supplied by OMIM] Mouse triggering receptor expressed on myeloid cells 1 (TREM-1), a 30kD member of the immunoglobulin superfamily expressed on neutrophils and a subset of monocytes. TREM-1 is involved in the microbial inflammatory response. It acts in conjunction with the adaptor protein DAP12 to activate neutrophils and monocytes, leading to the secretion of pro-inflammatory mediators and enhancement of the inflammatory response. Microbial infection also induces the release of soluble form of TREM-1 from monocytes, which is reported to act as an inhibitor of TREM-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-40907AF405
Alexa Fluor 488 Labeled NBP1-40907AF488
Alexa Fluor 647 Labeled NBP1-40907AF647
Alexa Fluor 700 Labeled NBP1-40907AF700
Allophycocyanin Labeled NBP1-40907APC
DyLight 350 Labeled NBP1-40907UV
DyLight 405 Labeled NBP1-40907V
DyLight 488 Labeled NBP1-40907G
DyLight 680 Labeled NBP1-40907FR
DyLight 755 Labeled NBP1-40907IR
PE Labeled NBP1-40907PE
PerCP Labeled NBP1-40907PCP

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Blogs on TREM-1.

Caspase 11: A novel non-canonical inflammasomes
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands. This regulated process then transmits downstream signals through adaptor molecules ending with the caspase cysteine proteases. Caspas...  Read full blog post.

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Bioinformatics

Gene Symbol TREM1
Entrez
Uniprot