TRAP1 Antibody

Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP2-20700] - Ovarian carcinoma. TRAP1 antibody [N1N3] dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: TRAP1 Antibody [NBP2-20700] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:3000.

Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [N1N3] (NBP2-20700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

TRAP1 antibody [N1N3] detects TRAP1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and permeabilized with 100% MeOH for 30 sec.Green: TRAP1 protein stained by TRAP1 antibody [N1N3] (NBP2-20700) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt, Po, Pm
• Applications: WB, ICC/IF, IHC, KO, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRAP1 Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit TRAP1 Antibody (NBP2-20700) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-TRAP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human TRAP1. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Mouse (91%), Rat (90%), Porcine (91%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Application Notes: Use in KD reported in scientific publication (PMID: 32664214).
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (89%), Zebrafish (81%), Chicken (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRAP1 Antibody

• HSP 75
• HSP75TNFR-associated protein 1
• HSP90Lheat shock protein 75 kDa, mitochondrial
• TNF receptor-associated protein 1
• TRAP-1
• tumor necrosis factor type 1 receptor associated protein
• Tumor necrosis factor type 1 receptor-associated protein

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TRAP1 Antibody (NBP2-20700)(1)

Publication using NBP2-20700

• Nguyen TTT, Zhang Y, Shang E et al. Inhibition of HDAC1/2 Along with TRAP1 Causes Synthetic Lethality in Glioblastoma Model Systems Cells 2020-07-10 [PMID: 32664214] (WB, KD, Human)

Bioinformatics

• Gene Symbol: TRAP1
• Uniprot:
  • Q12931(Human)