TRAP1 Antibody

Western Blot: TRAP1 Antibody [NBP1-32097] - Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: TRAP1 Antibody [NBP1-32097] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: TRAP1 protein stained by TRAP1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP1-32097] - Paraffin-embedded mouse intestine. TRAP1 antibody dilution: 1:500.

Western Blot: TRAP1 Antibody [NBP1-32097] - Sample(30 ug of whole cell lysate)A:Hep G2 B:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:1500.

Western Blot: TRAP1 Antibody [NBP1-32097] -Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, Func, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TRAP1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRAP1. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Rat (91%), Bovine (90%), Chimpanzee (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Proximity Ligation Assay Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (89%), Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRAP1 Antibody

• HSP 75
• HSP75TNFR-associated protein 1
• HSP90Lheat shock protein 75 kDa, mitochondrial
• TNF receptor-associated protein 1
• TRAP-1
• tumor necrosis factor type 1 receptor associated protein
• Tumor necrosis factor type 1 receptor-associated protein

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TRAP1