TRAP1 Antibody

Images

 
Western Blot: TRAP1 Antibody [NBP1-32097] - Sample(30 ug of whole cell lysate)A:Hep G2 B:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:1500.
Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody [NBP1-32097] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: TRAP1 protein stained by TRAP1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. ...read more
Immunohistochemistry-Paraffin: TRAP1 Antibody [NBP1-32097] - Paraffin-embedded mouse intestine. TRAP1 antibody dilution: 1:500.
Western Blot: TRAP1 Antibody [NBP1-32097] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.66 mg/ml

Order Details

TRAP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TRAP1. The exact sequence is proprietary.
Localization
Mitochondrion
Predicted Species
Rat (91%), Primate (100%), Bovine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%), Pig (89%), Chicken (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.66 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRAP1 Antibody

  • HSP 75
  • HSP75TNFR-associated protein 1
  • HSP90Lheat shock protein 75 kDa, mitochondrial
  • TNF receptor-associated protein 1
  • TRAP-1
  • tumor necrosis factor type 1 receptor associated protein
  • Tumor necrosis factor type 1 receptor-associated protein

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRAP1