Transthyretin/Prealbumin Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Transthyretin/Prealbumin Antibody [NBP1-89649] - Staining of human cell line CACO-2 shows localization to the Golgi apparatus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Transthyretin/Prealbumin Antibody [NBP1-89649] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: Transthyretin/Prealbumin Antibody [NBP1-89649] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.

Analysis in human plasma.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Transthyretin/Prealbumin Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TTR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Transthyretin/Prealbumin Protein (NBP1-89649PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Transthyretin/Prealbumin Antibody - BSA Free

• ATTR
• HsT2651
• PALB
• PALBTBPA
• Prealbumin
• prealbumin, amyloidosis type I
• TBPA
• thyroxine-binding prealbumin
• Transthyretin
• TTR

Background

Prealbumin is a serum protein usually occurring at 200-400mg/L serum. Prealbumin migrates faster than albumin in acidic starch gels and include alpha 1 antitrypsin, thyroxine binding prealbumin, and orosomucoid, an alpha 1 acid glycoprotein. The functions of prealbumin are to transport thyroxine, a task it shares with thyroxine binding globulin, retinol binding protein and vitamin A. Polymorphism of prealbumin is known in the mouse and pig.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transthyretin/Prealbumin Antibody (NBP1-89649)(3)

Publications using NBP1-89649

• Liu T, Zhang B, Jin X et al. Ophthalmic manifestations in a Chinese family with familial amyloid polyneuropathy due to a TTR Gly83Arg mutation. Eye (Lond) 2014-01-01 [PMID: 24113303] (IF/IHC, Human)
• Kato BS, Nicholson G, Neiman M et al. Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model. Proteome Sci 2011-11-17 [PMID: 22093360]
• Noborn F, O'Callaghan P, Hermansson E et al. Heparan sulfate/heparin promotes transthyretin fibrillization through selective binding to a basic motif in the protein. Proc Natl Acad Sci U S A 2011-04-01 [PMID: 21422279]

Bioinformatics

• Gene Symbol: TTR