Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]

Images

 
Western blot shows lysates of WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody ...read more
Fibroblast Activation Protein alpha /FAP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen ...read more
Fibroblast Activation Protein alpha /FAP was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP, KO
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
Leu26-Asp760
Accession # Q12884
Specificity
Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
FAP
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Knockout Validated 0.5 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3715 in the following applications:

Publications
Read Publications using
AF3715 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]

  • 170 kDa melanoma membrane-bound gelatinase
  • DKFZp686G13158
  • DPPIV
  • EC 3.4.21.-
  • FAP
  • FAPA
  • Fibroblast Activation Protein alpha
  • fibroblast activation protein, alpha
  • Integral membrane serine protease
  • Seprase
  • vibronectin

Background

FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.

  1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
  2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
  3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
  4. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
  5. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibroblast Activation Protein alpha/FAP Antibody (AF3715)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: CAR-T (Flow Cytometry), Flow Cytometry, ICC, IHC, IHC-P, Immunoprecipitation, Photoimmunotherapy, Western Blot.


Filter By Application
CAR-T (Flow Cytometry)
(1)
Flow Cytometry
(1)
ICC
(2)
IHC
(3)
IHC-P
(1)
Immunoprecipitation
(1)
Photoimmunotherapy
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(11)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF3715 Applications Species
M Friedrich, M Pohin, MA Jackson, I Korsunsky, SJ Bullers, K Rue-Albrec, Z Christofor, D Sathananth, T Thomas, R Ravindran, R Tandon, RS Peres, H Sharpe, K Wei, GFM Watts, EH Mann, A Geremia, M Attar, Oxford IBD, Roche Fibr, S McCuaig, L Thomas, E Collantes, HH Uhlig, SN Sansom, A Easton, S Raychaudhu, SP Travis, FM Powrie IL-1-driven stromal-neutrophil interactions define a subset of patients with inflammatory bowel disease that does not respond to therapies Nature Medicine, 2021;0(0):. 2021 [PMID: 34675383] (IHC, Human) IHC Human
M Shimizu, YI Koma, H Sakamoto, S Tsukamoto, Y Kitamura, S Urakami, K Tanigawa, T Kodama, N Higashino, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression Cancers, 2021;13(18):. 2021 [PMID: 34572779] (Western Blot, Human) Western Blot Human
N Ganig, F Baenke, ML Thepkayson, K Lin, VS Rao, FC Wong, H Polster, M Schneider, D Helm, M Pecqueux, AM Seifert, L Seifert, J Weitz, NN Rahbari, C Kahlert Proteomic Analyses of Fibroblast- and Serum-Derived Exosomes Identify QSOX1 as a Marker for Non-invasive Detection of Colorectal Cancer Cancers, 2021;13(6):. 2021 [PMID: 33802764] (Western Blot, Human) Western Blot Human
LE Bruijn, BEWM van den Ak, CM van Rhijn, JF Hamming, JHN Lindeman Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape J Am Heart Assoc, 2020;9(23):e017094. 2020 [PMID: 33190596] (ICC, Human) ICC Human
C Cho, R Mukherjee, AR Peck, Y Sun, N McBrearty, KV Katlinski, J Gui, PK Govindaraj, E Puré, H Rui, SY Fuchs Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intratumoral stromagenesis Oncogene, 2020;0(0):. 2020 [PMID: 32807917] (ICC, Human) ICC Human
N Shirasu, H Shibaguchi, H Yamada, M Kuroki, S Yasunaga Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies Cancer Cell Int., 2019;19(0):299. 2019 [PMID: 31787847] (Photoimmunotherapy, Human) Photoimmunotherapy Human
D Correa-Gal, D Jiang, S Christ, P Ramesh, H Ye, J Wannemache, S Kalgudde G, Q Yu, M Aichler, A Walch, U Mirastschi, T Volz, Y Rinkevich Patch repair of deep wounds by mobilized fascia Nature, 2019;0(0):. 2019 [PMID: 31776510] (IHC, Human) IHC Human
N Higashino, YI Koma, M Hosono, N Takase, M Okamoto, H Kodaira, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki Fibroblast activation protein-positive fibroblasts promote tumor progression through secretion of CCL2 and interleukin-6 in esophageal squamous cell carcinoma Lab. Invest., 2019;0(0):. 2019 [PMID: 30683902] (Human) Human
MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells Nat Commun, 2018;9(1):948. 2018 [PMID: 29507342] (Flow Cytometry, Mouse) Flow Cytometry Mouse
XP Chai, GL Sun, YF Fang, LH Hu, X Liu, XW Zhang Tumor-targeting efficacy of a BF211 prodrug through hydrolysis by fibroblast activation protein-? Acta Pharmacol. Sin., 2017;0(0):. 2017 [PMID: 29119969] (Western Blot, Mouse) Western Blot Mouse
Show All 15 Publications.

Review for Fibroblast Activation Protein alpha/FAP Antibody (AF3715) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3715:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Fibroblast Activation Protein alpha/FAP AF3715
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4
reviewed by:
Verified Customer
Immunohistochemistry-Paraffin Human 04/15/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast tumor
SpeciesHuman
LotZKW0618101

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Recent Reviews

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Verified Customer
04/15/2019
Application: Immunohistochemistry-Paraffin
Species: Human

Bioinformatics

Gene Symbol FAP
Uniprot