Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]

Images

 
Western blot shows lysates of WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody ...read more
Fibroblast Activation Protein alpha /FAP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen ...read more
Fibroblast Activation Protein alpha /FAP was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
Leu26-Asp760
Accession # Q12884
Specificity
Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
FAP
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3715 in the following applications:

Publications
Read Publications using
AF3715 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]

  • 170 kDa melanoma membrane-bound gelatinase
  • DKFZp686G13158
  • DPPIV
  • EC 3.4.21.-
  • FAP
  • FAPA
  • Fibroblast Activation Protein alpha
  • fibroblast activation protein, alpha
  • Integral membrane serine protease
  • Seprase
  • vibronectin

Background

FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.

  1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
  2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
  3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
  4. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
  5. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibroblast Activation Protein alpha/FAP Antibody (AF3715)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: CAR-T (Flow Cytometry), Flow Cytometry, IHC, IHC-P, Immunoprecipitation, Photoimmunotherapy, Western Blot.


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CAR-T (Flow Cytometry)
(1)
Flow Cytometry
(1)
IHC
(2)
IHC-P
(1)
Immunoprecipitation
(1)
Photoimmunotherapy
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF3715 Applications Species
N Shirasu, H Shibaguchi, H Yamada, M Kuroki, S Yasunaga Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies Cancer Cell Int., 2019;19(0):299. 2019 [PMID: 31787847] (Photoimmunotherapy, Human) Photoimmunotherapy Human
D Correa-Gal, D Jiang, S Christ, P Ramesh, H Ye, J Wannemache, S Kalgudde G, Q Yu, M Aichler, A Walch, U Mirastschi, T Volz, Y Rinkevich Patch repair of deep wounds by mobilized fascia Nature, 2019;0(0):. 2019 [PMID: 31776510] (IHC, Human) IHC Human
N Higashino, YI Koma, M Hosono, N Takase, M Okamoto, H Kodaira, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki Fibroblast activation protein-positive fibroblasts promote tumor progression through secretion of CCL2 and interleukin-6 in esophageal squamous cell carcinoma Lab. Invest., 2019;0(0):. 2019 [PMID: 30683902] (Human) Human
MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells Nat Commun, 2018;9(1):948. 2018 [PMID: 29507342] (Flow Cytometry, Mouse) Flow Cytometry Mouse
XP Chai, GL Sun, YF Fang, LH Hu, X Liu, XW Zhang Tumor-targeting efficacy of a BF211 prodrug through hydrolysis by fibroblast activation protein-? Acta Pharmacol. Sin., 2017;0(0):. 2017 [PMID: 29119969] (Western Blot, Mouse) Western Blot Mouse
A Lo, CP Li, EL Buza, R Blomberg, P Govindaraj, D Avery, J Monslow, M Hsiao, E Puré Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma JCI Insight, 2017;2(19):. 2017 [PMID: 28978805] (IHC-P, Human) IHC-P Human
AS Nagaraja, RL Dood, G Armaiz-Pen, Y Kang, SY Wu, JK Allen, NB Jennings, LS Mangala, S Pradeep, Y Lyons, M Haemmerle, KM Gharpure, NC Sadaoui, C Rodriguez-, C Ivan, Y Wang, K Baggerly, P Ram, G Lopez-Bere, J Liu, SC Mok, L Cohen, SK Lutgendorf, SW Cole, AK Sood Adrenergic-mediated increases in INHBA drive CAF phenotype and collagens JCI Insight, 2017;2(16):. 2017 [PMID: 28814667] (IHC, Mouse) IHC Mouse
X Feng, Q Wang, Y Liao, X Zhou, Y Wang, W Liu, G Zhang A synthetic urinary probe-coated nanoparticles sensitive to fibroblast activation protein ? for solid tumor diagnosis Int J Nanomedicine, 2017;12(0):5359-5372. 2017 [PMID: 28794628] (Western Blot, Human) Western Blot Human
MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice J. Biol. Chem., 2016;291(15):8070-89. 2016 [PMID: 26663085] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Kakarla S, Chow K, Mata M, Shaffer D, Song X, Wu M, Liu H, Wang L, Rowley D, Pfizenmaier K, Gottschalk S Antitumor effects of chimeric receptor engineered human T cells directed to tumor stroma. Mol Ther, 0;21(8):1611-20. 0 [PMID: 23732988] (CAR-T (Flow Cytometry), Human) CAR-T (Flow Cytometry) Human

Review for Fibroblast Activation Protein alpha/FAP Antibody (AF3715) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3715:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Fibroblast Activation Protein alpha/FAP AF3715
Enlarge
4
reviewed by:
Santhosh Sivajothi
IHC-P Human 04/15/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast tumor
SpeciesHuman
LotZKW0618101

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Biotin BAF3715

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Santhosh Sivajothi
04/15/2019
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol FAP
Uniprot