Transthyretin/Prealbumin Antibody (HL1823) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Transthyretin/Prealbumin Antibody (HL1823) - Azide and BSA Free [NBP3-25613] - Prealbumin antibody [HL1823] detects Prealbumin protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Prealbumin stained by Prealbumin antibody [HL1823] (NBP3-25613) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.

Immunohistochemistry-Paraffin: Transthyretin/Prealbumin Antibody (HL1823) - Azide and BSA Free [NBP3-25613] - Prealbumin antibody [HL1823] detects secreted Prealbumin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Prealbumin stained by Prealbumin antibody [HL1823] (NBP3-25613) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/Immunofluorescence: Transthyretin/Prealbumin Antibody (HL1823) - Azide and BSA Free [NBP3-25613] - Prealbumin antibody [HL1823] detects Prealbumin protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Prealbumin stained by Prealbumin antibody [HL1823] (NBP3-25613) diluted at 1:500. Blue: Fluoroshield with DAPI .

Western Blot: Transthyretin/Prealbumin Antibody (HL1823) - Azide and BSA Free [NBP3-25613] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Prealbumin antibody [HL1823] (NBP3-25613) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Transthyretin/Prealbumin Antibody (HL1823) - Azide and BSA Free [NBP3-25613] - Prealbumin antibody [HL1823] detects secreted Prealbumin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Prealbumin stained by Prealbumin antibody [HL1823] (NBP3-25613) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Human plasma (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Transthyretin/Prealbumin antibody [HL1823] (NBP3-25613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: HL1823
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Transthyretin/Prealbumin Antibody (HL1823) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Transthyretin/Prealbumin Antibody (HL1823) - Azide and BSA Free (NBP3-25613) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full lenngth human Transthyretin/Prealbumin
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TTR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:1000-1:10000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Transthyretin/Prealbumin Antibody (HL1823) - Azide and BSA Free

• ATTR
• HsT2651
• PALB
• PALBTBPA
• Prealbumin
• prealbumin, amyloidosis type I
• TBPA
• thyroxine-binding prealbumin
• Transthyretin
• TTR

Background

Prealbumin is a serum protein usually occurring at 200-400mg/L serum. Prealbumin migrates faster than albumin in acidic starch gels and include alpha 1 antitrypsin, thyroxine binding prealbumin, and orosomucoid, an alpha 1 acid glycoprotein. The functions of prealbumin are to transport thyroxine, a task it shares with thyroxine binding globulin, retinol binding protein and vitamin A. Polymorphism of prealbumin is known in the mouse and pig.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TTR