Transferrin Antibody - BSA Free

Western Blot: Transferrin Antibody [NB600-931] - Lane 1: molecular weight. Lane 2: Rabbit Antibody was diluted to 1:1000 for 2 hrs at RT. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight (TM) 649 conjugated (green), Goat IgG (H&L) Antibody DyLight (TM) 549 conjugated (red), Mouse IgG (H&L) Antibody DyLight (TM) 488 conjugated (blue)secondary antibody at 1:10,000 for 45 min at RT. Block: IRdye blocking buffer for 1hr at 4C.

Western Blot: Transferrin Antibody [NB600-931] - Lane 1: molecular weight. Lane 2: anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane.

Western Blot: Transferrin Antibody [NB600-931] - Lane 1: Human Transferrin. Lane 2: None.

Immunohistochemistry of Transferrin Antibody. Tissue: Proliferant, cycling human thymus. Antigen Retrieval: HIER pH6.2. Staining: diffuse cytoplasmic and membrane staining. Right: Transferrin antibody 209-4134 at 1:100. Left: Normal Rabbit IgG Neg Ctrl.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

Transferrin Antibody - BSA Free Summary

• Description: This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Transferrin [Human Serum]
  
  Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Transferrin Antibody was produced by repeated immunizations with human serum Transferrin protein. (Uniprot: P02787)
• Localization: Secreted
• Specificity: Cross reactivity against Transferrin from other tissues and species may occur but have not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TF
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Western Blot 1:2000-1:10000
• Application Notes: This product has been tested in western blotting, ELISA and IHC. Researchers should determine optimal titers for applications that are not stated below.

Reactivity Notes

Cross reactivity against Transferrin from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Transferrin Antibody - BSA Free

• Beta-1 metal-binding globulin
• DKFZp781D0156
• EC 3.4.21
• PRO1557
• PRO2086
• Serotransferrin
• Siderophilin
• TF
• Transferrin

Background

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TF
• Entrez:
  • 7018(Human)
• Uniprot:
  • P02787(Human)