Human TFPI Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human TFPI Quantikine ELISA Kit Summary

• Background: The Quantikine Human TFPI Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human TFPI in cell culture supernates, plasma, and urine. It contains NS0-expressed recombinant human TFPI and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFPI showed linear curves that were parallel to the standard curves obtained using the Quant...ikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFPI.
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• Specificity: Natural and recombinant human TFPI. This kit recognizes predominantly free TFPI and a very small percentage of the LDL- and HDL-bound TFPI.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TFPI

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TFPI Quantikine ELISA Kit

• convertin
• EPI
• EPITFPI1LACITFI
• Extrinsic pathway inhibitor
• LACI
• Lipoprotein-associated coagulation inhibitor
• TFPI
• tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• tissue factor pathway inhibitor

Background

Tissue Factor Pathway Inhibitor (TFPI) is a key regulator of the extrinsic coagulation pathway. It inhibits coagulation through the formation of a quaternary complex with Coagulation Factor X, Coagulation Factor III/Tissue Factor, and Coagulation Factor VII, thereby preventing the activation of Factor X. TFPI also exhibits anti-angiogenic and anti-metastatic effects. It is produced by vascular endothelial cells and circulates as a free molecule, on platelet surfaces, and in complex with LDL and HDL particles. Abnormal TFPI concentrations in the circulation are associated with thrombosis, disseminated intravascular coagulation, and sepsis. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TFPI (DTFP10)(13)

Publications using DTFP10

• Hellum, M;Sletten, M;Le, MS;Stavseth, V;Glosli, H;Backe, PH;Iversen, N;Henriksson, CE; Characterization of a novel factor X variant, p.F139L, associated with bleedings in heterozygous and compound heterozygous form Thrombosis research 2025-09-01 [PMID: 40763424] (Human)
• Li, X;Song, X;Mahmood, DFD;Sim, MMS;Bidarian, SJ;Wood, JP; Activated protein C, protein S, and tissue factor pathway inhibitor cooperate to inhibit thrombin activation Thrombosis research 2023-08-23 [PMID: 37660436] (Human)
• Ghanbari, EP;Jakobs, K;Puccini, M;Reinshagen, L;Friebel, J;Haghikia, A;Kr�nkel, N;Landmesser, U;Rauch-Kr�hnert, U; The Role of NETosis and Complement Activation in COVID-19-Associated Coagulopathies Biomedicines 2023-05-05 [PMID: 37239041] (Human)
• VE Fiestas So, NR da Costa F, CF Dos Santos, G Corrêa, MDC Cipitelli, M Dornelas R, LJ de Souza, PV Damasco, RV da Cunha, FB Dos Santos, LM de Oliveir, EL de Azeredo Different Profiles of Cytokines, Chemokines and Coagulation Mediators Associated with Severity in Brazilian Patients Infected with Dengue Virus Viruses, 2021-09-08;13(9):. 2021-09-08 [PMID: 34578370] (Human)
• LE Landgrebe, CB Juhl, V Andersen, L Moitinho-S, C Bang, EM Bladbjerg Postprandial factor VII activation does not increase plasma concentrations of prothrombin fragment 1�+�2 in patients with morbid obesity Thromb. Res., 2020-09-01;196(0):260-267. 2020-09-01 [PMID: 32920297] (Human)
• S Chang, D Biltoft, A Skakkebæk, J Fedder, A Bojesen, MV Bor, CH Gravholt, AB Münster Testosterone treatment and association with thrombin generation and coagulation inhibition in Klinefelter syndrome: A cross-sectional study Thromb. Res., 2019-08-19;182(0):175-181. 2019-08-19 [PMID: 31505312] (Human)
• H Schöchl, M van Griens, S Heitmeier, V Laux, U Kipman, J Roodt, S Bahrami, H Redl Dual inhibition of thrombin and activated factor X attenuates disseminated intravascular coagulation and protects organ function in a baboon model of severe Gram-negative sepsis Crit Care, 2017-03-13;21(1):51. 2017-03-13 [PMID: 28288667] (Primate - Papio ursinus (Chacma baboon))
• E Leal de Az, VE Solórzano, DB de Oliveir, CF Marinho, LJ de Souza, RV da Cunha, PV Damasco, CF Kubelka, LM de-Oliveir Increased circulating procoagulant and anticoagulant factors as TF and TFPI according to severity or infecting serotypes in human dengue infection Microbes Infect, 2016-08-31;19(1):62-68. 2016-08-31 [PMID: 27592310] (Human)
• Annette Eyking MiR-205 and MiR-373 Are Associated with Aggressive Human Mucinous Colorectal Cancer PLoS ONE, 2016-06-06;11(6):e0156871. 2016-06-06 [PMID: 27271572] (Human)
• Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation Sci Rep, 2016-05-04;6(0):25171. 2016-05-04 [PMID: 27142573] (Human)
• The C-terminus of tissue factor pathway inhibitor alpha is required for its interaction with factors V and Va. J. Thromb. Haemost., 2012-09-01;10(9):1944-6. 2012-09-01 [PMID: 22738072] (Human)
• Girard TJ, Tuley E, Broze GJ TFPIbeta is the GPI-anchored TFPI isoform on human endothelial cells and placental microsomes. Blood, 2011-12-05;119(5):1256-62. 2011-12-05 [PMID: 22144186] (Human)
• Kentsis A, Bradwin G, Miller DT, Trenor CC Venous thrombosis associated with gene deletion of tissue factor pathway inhibitor. Am. J. Hematol., 2009-11-01;84(11):775-6. 2009-11-01 [PMID: 19806663] (Human)

FAQs for TFPI (DTFP10). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TFPI