Human TFPI Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human TFPI Quantikine ELISA Kit Summary

• Background: The Quantikine Human TFPI Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human TFPI in cell culture supernates, plasma, and urine. It contains NS0-expressed recombinant human TFPI and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFPI showed linear curves that were parallel to the standard curves obtained using the Quant...ikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFPI.
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• Specificity: Natural and recombinant human TFPI. This kit recognizes predominantly free TFPI and a very small percentage of the LDL- and HDL-bound TFPI.
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TFPI

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TFPI Quantikine ELISA Kit

• convertin
• EPI
• EPITFPI1LACITFI
• Extrinsic pathway inhibitor
• LACI
• Lipoprotein-associated coagulation inhibitor
• TFPI
• tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• tissue factor pathway inhibitor

Background

Tissue Factor Pathway Inhibitor (TFPI) is a key regulator of the extrinsic coagulation pathway. It inhibits coagulation through the formation of a quaternary complex with Coagulation Factor X, Coagulation Factor III/Tissue Factor, and Coagulation Factor VII, thereby preventing the activation of Factor X. TFPI also exhibits anti-angiogenic and anti-metastatic effects. It is produced by vascular endothelial cells and circulates as a free molecule, on platelet surfaces, and in complex with LDL and HDL particles. Abnormal TFPI concentrations in the circulation are associated with thrombosis, disseminated intravascular coagulation, and sepsis. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TFPI (DTFP10)(11)

Publications using DTFP10

• Ghanbari, EP;Jakobs, K;Puccini, M;Reinshagen, L;Friebel, J;Haghikia, A;Kr�nkel, N;Landmesser, U;Rauch-Kr�hnert, U; The Role of NETosis and Complement Activation in COVID-19-Associated Coagulopathies Biomedicines 2023-05-05 [PMID: 37239041] (Human)
• VE Fiestas So, NR da Costa F, CF Dos Santos, G Corrêa, MDC Cipitelli, M Dornelas R, LJ de Souza, PV Damasco, RV da Cunha, FB Dos Santos, LM de Oliveir, EL de Azeredo Different Profiles of Cytokines, Chemokines and Coagulation Mediators Associated with Severity in Brazilian Patients Infected with Dengue Virus Viruses, 2021-09-08;13(9):. 2021-09-08 [PMID: 34578370] (Human)
• LE Landgrebe, CB Juhl, V Andersen, L Moitinho-S, C Bang, EM Bladbjerg Postprandial factor VII activation does not increase plasma concentrations of prothrombin fragment 1�+�2 in patients with morbid obesity Thromb. Res., 2020-09-01;196(0):260-267. 2020-09-01 [PMID: 32920297] (Human)
• S Chang, D Biltoft, A Skakkebæk, J Fedder, A Bojesen, MV Bor, CH Gravholt, AB Münster Testosterone treatment and association with thrombin generation and coagulation inhibition in Klinefelter syndrome: A cross-sectional study Thromb. Res., 2019-08-19;182(0):175-181. 2019-08-19 [PMID: 31505312] (Human)
• H Schöchl, M van Griens, S Heitmeier, V Laux, U Kipman, J Roodt, S Bahrami, H Redl Dual inhibition of thrombin and activated factor X attenuates disseminated intravascular coagulation and protects organ function in a baboon model of severe Gram-negative sepsis Crit Care, 2017-03-13;21(1):51. 2017-03-13 [PMID: 28288667] (Primate - Papio ursinus (Chacma baboon))
• E Leal de Az, VE Solórzano, DB de Oliveir, CF Marinho, LJ de Souza, RV da Cunha, PV Damasco, CF Kubelka, LM de-Oliveir Increased circulating procoagulant and anticoagulant factors as TF and TFPI according to severity or infecting serotypes in human dengue infection Microbes Infect, 2016-08-31;19(1):62-68. 2016-08-31 [PMID: 27592310] (Human)
• Annette Eyking MiR-205 and MiR-373 Are Associated with Aggressive Human Mucinous Colorectal Cancer PLoS ONE, 2016-06-06;11(6):e0156871. 2016-06-06 [PMID: 27271572] (Human)
• Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation Sci Rep, 2016-05-04;6(0):25171. 2016-05-04 [PMID: 27142573] (Human)
• The C-terminus of tissue factor pathway inhibitor alpha is required for its interaction with factors V and Va. J. Thromb. Haemost., 2012-09-01;10(9):1944-6. 2012-09-01 [PMID: 22738072] (Human)
• Girard TJ, Tuley E, Broze GJ TFPIbeta is the GPI-anchored TFPI isoform on human endothelial cells and placental microsomes. Blood, 2011-12-05;119(5):1256-62. 2011-12-05 [PMID: 22144186] (Human)
• Kentsis A, Bradwin G, Miller DT, Trenor CC Venous thrombosis associated with gene deletion of tissue factor pathway inhibitor. Am. J. Hematol., 2009-11-01;84(11):775-6. 2009-11-01 [PMID: 19806663] (Human)

Bioinformatics

• Gene Symbol: TFPI