TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free

Western Blot: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Analysis using TNFSF11 mAb against HEK293 (1) and TNFSF11 (AA: 74-308)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Analysis of Hela cells using TNFSF11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Analysis of bladder cancer tissues using TNFSF11 mouse mAb with DAB staining.

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Analysis of Hela cells using TNFSF11 mouse mAb (green) and negative control (red).

Western Blot: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Analysis using TNFSF11 mAb against human TNFSF11 (AA: 74-308) recombinant protein. (Expected MW is 52.6 kDa)

Western Blot: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Analysis using TNFSF11 mouse mAb against COS7 (1), Hela (2), U937 (3), HL-60 (4), Raji (5), Ramos (6), Jurkat (7), and SW480 (8) cell lysate.

ELISA: TRANCE/TNFSF11/RANK L Antibody (8A7B9) [NBP2-61813] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 8A7B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free Summary

• Description: Novus Biologicals Mouse TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free (NBP2-61813) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-TRANCE/TNFSF11/RANK L Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human TRANCE/TNFSF11/RANK L (AA: 74-308) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF11
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 35.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (8A7B9) - BSA Free

• CD254 antigen
• CD254
• ODF
• OPGL
• OPGLOPTB2
• Osteoclast differentiation factor
• Osteoprotegerin ligand
• RANK L
• RANKL
• RANKLreceptor activator of nuclear factor kappa B ligand
• Receptor activator of nuclear factor kappa-B ligand
• sOdf
• TNF-related activation-induced cytokine
• TNFSF11
• TRANCE
• TRANCEODFhRANKL2
• tumor necrosis factor (ligand) superfamily, member 11
• tumor necrosis factor ligand superfamily member 11

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRANCE/TNFSF11/RANK L Antibody (NBP2-61813)(1)

Publication using NBP2-61813

• Marcadet L, Juracic ES, Khan N et al. RANKL Inhibition Reduces Cardiac Hypertrophy in mdx Mice and Possibly in Children with Duchenne Muscular Dystrophy Cells 2023-06-03 [PMID: 37296659] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: TNFSF11