TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free


Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - MQDL detects EMT biomarkers in clinical prostate cancer. EMT biomarkers (EpCAM, N-Cad, and RANKL)were detected in a clinical primary prostate more
Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - Analysis using Azide Free version of NB100-56512. Human lymph node lysate (35ug per lane, RIPA buffer). Band detected at ~35kDa and ~28kDa. (Expected more
Immunocytochemistry/ Immunofluorescence: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - TRANCE was detected in immersion fixed mouse splenocytes using anti-human/mouse/rat mouse monoclonal antibody (Catalog # more
Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - MQDL detects EMT biomarkers in clinical bone tissue specimens. A representative specimen of human prostate cancer bone metastasis more
Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - Analysis of transfected cell lysate was probed with TRANCE/TNFSF11/RANK L antibody.
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - Analysis using Azide Free version of NB100-56512. FFPE human liver stained with TRANCE/TNFSF11/RANK L antibody, peroxidase-conjugate more
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) [NB100-56512] - Analysis using Azide Free version of NB100-56512. FFPE human lymph node probed with TRANCE/TNFSF11/RANK L antibody.
MQDL detects EMT biomarkers in clinical bone tissue specimens.A representative specimen of human prostate cancer bone metastasis co-expressed high levels of epithelial EpCAM, and mesenchymal RANKL and vimentin proteins. more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, ChIP
BSA Free
1.0 mg/ml

Order Details

TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free Summary

A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE/TNFSF11/RANK L was used as immunogen.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • ELISA reported in scientific literature (PMID 15935726)
  • Flow (Cell Surface)
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-2 ug/ml
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
TRANCE/TNFSF11/RANK L Overexpression Lysate
Read Publications using
NB100-56512 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (12A668) - BSA Free

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11


TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. TRANCE/RANKL is also important in T and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRANCE/TNFSF11/RANK L Antibody (NB100-56512)(38)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Guinea Pig.

We have publications tested in 8 applications: Chemotaxis, ELISA, Flow-CS, ICC/IF, IF/IHC, IHC, IHC-P, WB.

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Guinea Pig
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Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using NB100-56512 Applications Species
Odo A, Kunimatsu R, Abe T et al. Stem cells derived from human exfoliated deciduous teeth-based media in a rat root resorption model Archives of Oral Biology 2023-11-01 [PMID: 38056228] (IHC, Rat) IHC Rat
Ding Y, Yang Y, Xu F et al. Early protection against bone stress injuries by mobilization of endogenous targeted bone remodeling iScience 2023-09-15 [PMID: 37664634] (ICC/IF) ICC/IF
Leite FRM, de Aquino SG, Guimaraes MR. RANKL expression is differentially modulated by TLR2 and TLR4 signaling in fibroblasts and osteoblasts Immunology Innovation. (WB, Mouse, Rat)

RANKL antibody used for WB for detecting the protein expression of receptor activator of nuclear factor-kappa B ligand /RANKL induced by E coli lipopolysaccharide/LPS, Porphyromonas gingivalis LPS and interleukin-1 beta in Mouse periodontal ligament fibroblasts (mPDL; immortalized with simian virus 40 large T antigen) and Osteoblastic rat osteosarcoma cells ROS 17/2.8 (ROS)
WB Mouse, Rat
Hild V, Mellert K, Möller P, Barth TFE Giant Cells of Various Lesions Are Characterised by Different Expression Patterns of HLA-Molecules and Molecules Involved in the Cell Cycle, Bone Metabolism, and Lineage Affiliation: An Immunohistochemical Study with a Review of the Literature Cancers 2023-07-21 [PMID: 37509363] (IHC-P, Human)

1:400 IHC-P dilution
IHC-P Human
Wei F, Tuong ZK, Omer M et al. A novel multifunctional radioprotective strategy using P7C3 as a countermeasure against ionizing radiation-induced bone loss Bone research 2023-06-29 [PMID: 37385982] (IHC-P, Rat) IHC-P Rat
Liu J, Yue J, Wang K et al. Tertiary Lymphoid Structures Are Related to Inflammatory Progression and Bone Loss in Human Apical Periodontitis Journal of endodontics 2023-06-17 [PMID: 37331649]
Kunimatsu R, Kimura A, Sakata S et al. Effects of baicalin on the proliferation and expression of OPG and RANKL in human cementoblast-lineage cells J Dent Sci 2022-01-14 [PMID: 35028034]
Lysitska A, Galanis N, Skandalos I Et al. Histology and Immunohistochemistry of Radial Arteries Are Suggestive of an Interaction between Calcification and Early Atherosclerotic Lesions in Chronic Kidney Disease Medicina 2021-10-24 [PMID: 34833374] (IHC-P, Human) IHC-P Human
Sengun MC, Gunpinar S Effects of systemic hydroxytyrosol application in experimental periodontitis of rats International Journal of Plant Based PHARMACEUTICALS 2022-01-01 (IF/IHC, Rat) IF/IHC Rat
Liu X, Zhu R, Luo Y et al. Distinct human Langerhans cell subsets orchestrate reciprocal functions and require different developmental regulation Immunity 2021-09-08 [PMID: 34508661]
Show All 38 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRANCE/TNFSF11/RANK L Antibody (NB100-56512). (Showing 1 - 3 of 3 FAQs).

  1. We have received a customer inquiry regarding your RANKL Antibody (12A668) 0.1mg, Cat # NB100-56512 (previously from Imgenex). Specifically, they would like to know if this product is suitable for use in immunoprecipitation, also, what is the level of cross reactivity to mouse compared to the target reactivity?
    • This product has been validated in mouse, so it is 100% guaranteed for use on mouse samples. It has never been directly tested by Novus or Imgenex for IP, but it works in ICC and for surface staining in Flow Cytometry. Because of this, I would expect that it would be suitable for use in IP as well.
  2. I need a positive control in order to establish them for IHC-P. Do you have any further recommendations except the ones stated on the website. For example what kind of tissue could be used as a positive control?
    • RANK/TNFRSF11A demonstrates ubiquitous expression with high levels found in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. You may use any of these tissues as positive controls. For additional options, you may refer to the staining results posted at Human Protein atlas database and in some IHC-P related publications at online product sheet.
  3. Does your RANKL antibody (NB100-56512, Clone 12A668) block the ligand binding site? (or what the epitope range is?) Furthermore, can this antibody use as agonistic?
    • This antibody was raised against a bacterially expressed fusion protein containing amino acid residues 1-317 / whole sequence of mouse TRANCE was used as immunogen (NP_003692; uniprot site). We have not epitope mapped this antibody and accordingly, we are not sure where on RANKL does this antibody bind. We are not aware of its use as agonist or antigonist but since this antibody worked really well in Flow, ICC/IF and IHC-P (applications wherein the antibody binds the antigen in more of native state), it is quite possible that it may work for functional application under in-vitro or in-vivo conditions.

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Secondary Antibodies


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The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Gene Symbol TNFSF11