TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free


Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Analysis of human lymph node lysate (35ug per lane, RIPA buffer) at 0.5ug/ml. Band detected at ~35kDa and ~28kDa. (Expected MW of more
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Formalin-fixed, paraffin-embedded human lymph node probed with Trance antibody at 5 ug/ml. Staining of more
Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Analysis of transfected cell lysate was probed with 2 ug of Trance antibody.
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Formalin-fixed, paraffin-embedded human liver stained with Trance antibody (1:500, 1 ug/ml), peroxidase-conjugate and more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Azide Free
1.0 mg/ml

TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free Summary

A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen (NP_003692).
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1-2 ug/ml
  • Chromatin Immunoprecipitation 1:10-1:500
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 5 ug/ml
  • CyTOF-ready
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Use in chromatin immunoprecipitation reported in scientific literature (PMID 25859979) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
TRANCE/TNFSF11/RANK L Lysate (NBL1-17162)
Read Publications using
NB100-56593 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Sterile - filtered PBS
No Preservative
1.0 mg/ml
Protein G purified

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11


TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells (1-2). RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). TRANCE/RANKL is also important in T and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (4). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
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Applications: Flow, IHC, IHC-P, PAGE, IF
Species: Hu, Mu, Rt
Applications: WB, ELISA
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Applications: IHC, IHC-P
Species: Hu
Species: Hu, Mu, Rt, Po, Ch, Rb
Applications: WB, IHC, IP
Species: Hu, Rb
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Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
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Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP
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Publications for TRANCE/TNFSF11/RANK L Antibody (NB100-56593)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-56593 Applications Species
Tanaka T, Kelly M, Takei Y, Yamanouchi D. RANKL-mediated osteoclastogenic differentiation of macrophages in the abdominal aorta of angiotensin II-infused apolipoprotein E knockout mice. J Vasc Surg Apr 20 2018 [PMID: 29685509] (ICC/IF, Mouse)

This reference was from the PE version of this antibody.
ICC/IF Mouse
Hu P, Chu GC, Zhu G et al. Multiplexed quantum dot labeling of activated c-Met signaling in castration-resistant human prostate cancer. PLoS One. 2011 [PMID: 22205960]
Shin M, Matsuo K, Tada T et al. The inhibition of RANKL/RANK signaling by osteoprotegerin suppresses bone invasion by oral squamous cell carcinoma cells. Carcinogenesis. 2011 Nov [PMID: 21890459]
Parrula C, Zimmerman B, Nadella P et al. Expression of tumor invasion factors determines systemic engraftment and induction of humoral hypercalcemia in a mouse model of adult T-cell leukemia. Vet Pathol. 2009 Sep [PMID: 19429977]
Ghoreishi M, Bach P, Obst J et al. Expansion of antigen-specific regulatory T cells with the topical vitamin d analog calcipotriol. J Immunol. 2009 May 15 [PMID: 19414758]
Sandra F, Hendarmin L, Kukita T et al. Ameloblastoma induces osteoclastogenesis: a possible role of ameloblastoma in expanding in the bone. Oral Oncol. 2005 Jul [PMID: 15935726]
Rubin J, Chung LW, Fan X et al. Prostate carcinoma cells that have resided in bone have an upregulated IGF-I axis. Prostate. 2004 Jan 1 [PMID: 14673951]
Fiumara P, Snell V, Li Y et al. Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines. Blood. 2001 Nov 1 [PMID: 11675352]

Antibodies cited: 1. OPG (IMG-103), WB: Fig 1A [human Hodgkin/Reed-Sternberg (H/RS) cell lines}, Fig 1B (human lymphoid tumors), Table 1 (multiple human hematopoietic cell lines) 2. Rank/TNF11aR/ODFR (IMG-128), WB: Fig 1A (human H/RS cell lines), Fig 1B (
Pearse RN, Sordillo EM, Yaccoby S et al. Multiple myeloma disrupts the TRANCE/ osteoprotegerin cytokine axis to trigger bone destruction and promote tumor progression. Proc Natl Acad Sci U S A. 2001 Sep 25 [PMID: 11562486]

Antibodies cited [IHC (paraffin)], Figs 1 and 4 (human plasmocytoma and decalified bone marrow): 1. OPG (IMG-103) 2. Trance/RankL/OPGL/ODF (IMG-133).
Kikuchi T, Matsuguchi T, Tsuboi N et al. Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via Toll-like receptors. J Immunol. 2001 Mar 1 [PMID: 11207318]
Show All 11 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TRANCE/TNFSF11/RANK L Antibody (NB100-56593). (Showing 1 - 1 of 1 FAQs).

  1. I would appreciate if you are able to provide the immunogen sequence for this antibody.
    • Our RANKL antibody with catalogue number NB100-56593 was raised against a bacterially expressed fusion protein containing amino acid residues 1-317 of mouse RANKL

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56593AF405
Alexa Fluor 488 Labeled NB100-56593AF488
Alexa Fluor 647 Labeled NB100-56593AF647
Alexa Fluor 700 Labeled NB100-56593AF700
Allophycocyanin Labeled NB100-56593APC
DyLight 350 Labeled NB100-56593UV
DyLight 405 Labeled NB100-56593V
DyLight 488 Labeled NB100-56593G
DyLight 550 Labeled NB100-56593R
DyLight 650 Labeled NB100-56593C
DyLight 680 Labeled NB100-56593FR
DyLight 755 Labeled NB100-56593IR
HRP Labeled NB100-56593H
PE Labeled NB100-56593PE
PerCP Labeled NB100-56593PCP

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The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Gene Symbol TNFSF11