TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.

Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.

Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis of FFPE human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz. Image using the standard format of this product.

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis in RAW cells using 2 ug of PE conjugated antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody.

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis using the Alexa Fluor (R) 647 conjugate. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 12A380
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free Summary

• Immunogen: A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.
• Localization: Flow Cytometry (Surface): please see Yoneda et al. 2004 for details. Immunohistochemistry-Frozen: please see Yoneda et al. 2004 for details.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF11
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 1ul/1 million cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:2000
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 18705798)
  • Western Blot 1-5ug/ml
• Control:

  TRANCE/TNFSF11/RANK L Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free

• CD254 antigen
• CD254
• ODF
• OPGL
• OPGLOPTB2
• Osteoclast differentiation factor
• Osteoprotegerin ligand
• RANK L
• RANKL
• RANKLreceptor activator of nuclear factor kappa B ligand
• Receptor activator of nuclear factor kappa-B ligand
• sOdf
• TNF-related activation-induced cytokine
• TNFSF11
• TRANCE
• TRANCEODFhRANKL2
• tumor necrosis factor (ligand) superfamily, member 11
• tumor necrosis factor ligand superfamily member 11

Background

TRANCE/OPGL/RANkL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANkL {receptor activator of NF-kB (RANk) ligand} has been implicated in interactions between T cells and dendritic cells (4-5). RANk ligand (RANkL/TRANCE) binds to RANk on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (4). TRANCE/RANkL is also important in T- and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (1,2). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRANCE/TNFSF11/RANK L Antibody (NBP2-27402)(4)

Publications using NBP2-27402

• Kragstrup Tue Wenzel, Greisen Stinne Ravn, Nielsen Morten Aagaard et al. The interleukin-20 receptor axis in early rheumatoid arthritis: novel links between disease-associated autoantibodies and radiographic progression. Arthritis Research & Therapy 2016-03-11 [PMID: 26968800] (FLOW, Human)
  
  Details:
  This citation used the Azide Free version of this antibody.
• Walker CG, Ito Y, Dangaria S et al. RANKL, osteopontin, and osteoclast homeostasis in a hyperocclusion mouse model. Eur J Oral Sci. 2008-08-01 [PMID: 18705798] (IHC-P, Human)
  
  Details:
  Trance/RANkL/OPGL/ODF (IMG-185A). IHC (paraffin): Swiss Webster mouse mandible tissue, Fig 4.
• Shetty R, Pepin A, Charest A et al. Expression of bone-regulatory proteins in human valve allografts. Heart. 2006-09-01 [PMID: 16449517] (IHC-Fr)
• Yoneda T, Ishimaru N, Arakaki R et al. Estrogen deficiency accelerates murine autoimmune arthritis associated with receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenesis. Endocrinology. 2004-05-01 [PMID: 14726437] (IHC-Fr, Flow-CS)
  
  Details:
  Flow Cytometry-cell surface staining: Figure 4A; (Immunohistochemistry-frozen sections: Figure 4E) for IMG-185A.

Bioinformatics

• Gene Symbol: TNFSF11
• Entrez:
  • 21943(Mouse)