TRANCE/TNFSF11/RANK L Antibody (12A380)

Images

 
Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using Azide Free version of NB100-56748. Recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer ...read more
Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using Azide Free version of NB100-56748. RAW cells using 2 ug of PE conjugated antibody. Shaded histogram represents cells without ...read more
Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.
Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ...read more
Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P
Clone
12A380
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

TRANCE/TNFSF11/RANK L Antibody (12A380) Summary

Immunogen
A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFSF11
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 4-6 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:50 - 1:250
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:250
Application Notes
Flow Cytometry (Surface): please see Yoneda et al. 2004 for details. Immunohistochemistry-Frozen: please see Yoneda et al. 2004 for details.
Positive Control
TRANCE/TNFSF11/RANK L Lysate (NBL1-17162)
Publications
Read Publications using
NB100-56748 in the following applications:

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (12A380)

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • OPGLOPTB2
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKL
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • TRANCE
  • TRANCEODFhRANKL2
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11

Background

TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. TRANCE/RANKL is also important in T- and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TRANCE/TNFSF11/RANK L Antibody (NB100-56748). (Showing 1 - 1 of 1 FAQs).

  1. Has it been tested blocking/neutrazing capabilities block ligand-receptor interaction?
    • Our product NB100-56748 has unfortunately not been tested for blocking or neutralization yet, so we do not know if it will block the ligand/receptor interactions.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP2-27389
Alexa Fluor 488 Labeled NBP1-51817AF488
Alexa Fluor 647 Labeled NBP2-27390
Alexa Fluor 647 Labeled NBP1-51817AF647
Allophycocyanin Labeled NBP2-27402APC
DyLight 350 Labeled NBP2-27402UV
DyLight 405 Labeled NBP2-27402V
DyLight 488 Labeled NBP2-27402G
DyLight 550 Labeled NBP2-27402R
DyLight 650 Labeled NBP2-27402C
DyLight 680 Labeled NBP2-27402FR
DyLight 755 Labeled NBP2-27402IR
HRP Labeled NBP2-27402H
PerCP Labeled NBP2-27402PCP

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Bioinformatics Tool for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)

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Diseases for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)

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Research Areas for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)

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Blogs on TRANCE/TNFSF11/RANK L.

The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Bioinformatics

Gene Symbol TNFSF11
Entrez