TRANCE/TNFSF11/RANK L Antibody (12A380) - BSA Free

Images

 
Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of FFPE human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved ...read more
Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ...read more
Immunohistochemistry-Frozen: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis in RAW cells using 2 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control ...read more
Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
12A380
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

TRANCE/TNFSF11/RANK L Antibody (12A380) - BSA Free Summary

Immunogen
A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFSF11
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Cell Surface)
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:250
  • Western Blot 4-6 ug/ml
Application Notes
Flow Cytometry (Surface) and Immunohistochemistry-Frozen: see Yoneda et al. 2004 for details. Use in Flow-cell surface reported in scientific literature (PMID: 14726437). Use in ICC/IF was reported in scientific literature (PMID: 14726437).
Control
TRANCE/TNFSF11/RANK L Overexpression Lysate
Publications
Read Publications using
NB100-56748 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (12A380) - BSA Free

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • OPGLOPTB2
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKL
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • TRANCE
  • TRANCEODFhRANKL2
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11

Background

TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. TRANCE/RANKL is also important in T- and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56508
Species: Av, Hu, Mu, Rt
Applications: CyTOF-ready, Flow-CS, Flow, IHC, IHC-P, WB
DY805
Species: Hu
Applications: ELISA
210-TA
Species: Hu
Applications: BA
D6050
Species: Hu
Applications: ELISA
DCDL40
Species: Hu
Applications: ELISA
NB100-56173
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
MAB1419
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
H00000054-D01P
Species: Hu, Mu
Applications: IHC, IHC-P, WB
H00057410-M02
Species: Hu, Mu, Rt
Applications: ELISA, S-ELISA, WB
NBP1-87315
Species: Hu
Applications: IHC, IHC-P
MAB7665
Species: Hu
Applications: IHC, WB
H00001513-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
NB100-56732
Species: Hu, Mu
Applications: ICC/IF, IHC, Simple Western, WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
NB300-164
Species: Ca, Hu, Mu, Po, Pm, Rt(-)
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
NB100-56748
Species: Hu, Mu
Applications: WB, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IHC-P
Supplier Logo

Publications for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow, Flow-CS, IHC-Fr, IHC-P.


Filter By Application
Flow
(1)
Flow-CS
(1)
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-56748 Applications Species
Lei L, Huang Z, Feng J et Al. Loss of receptor tyrosine kinase-like orphan receptor 2 impairs the osteogenesis of mBMSCs by inhibiting signal transducer and activator of transcription 3 Stem Cell Res Ther Mar 26 2020 [PMID: 32216811]
Delgado-Calle J, Hancock B, Likine EF et al. MMP14 is a novel target of PTH signaling in osteocytes that controls resorption by regulating soluble RANKL production. FASEB J 2018 May [PMID: 29401593]
Kragstrup Tue Wenzel, Greisen Stinne Ravn, Nielsen Morten Aagaard et al. The interleukin-20 receptor axis in early rheumatoid arthritis: novel links between disease-associated autoantibodies and radiographic progression. Arthritis Research & Therapy 2016 Mar 11 [PMID: 26968800] (Flow, Human)

Details:
This citation used the Azide Free version of this antibody.
Flow Human
Walker CG, Ito Y, Dangaria S et al. RANKL, osteopontin, and osteoclast homeostasis in a hyperocclusion mouse model. Eur J Oral Sci. 2008 Aug [PMID: 18705798] (IHC-P, Mouse)

Details:
Trance/RANkL/OPGL/ODF (IMG-185A). IHC (paraffin): Swiss Webster mouse mandible tissue, Fig 4.
IHC-P Mouse
Shetty R, Pepin A, Charest A et al. Expression of bone-regulatory proteins in human valve allografts. Heart. 2006 Sep [PMID: 16449517] (IHC-Fr) IHC-Fr
Yoneda T, Ishimaru N, Arakaki R et al. Estrogen deficiency accelerates murine autoimmune arthritis associated with receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenesis. Endocrinology. 2004 May [PMID: 14726437] (Flow-CS, IHC-Fr)

Details:
(Flow Cytometry-cell surface staining: Figure 4A); (Immunohistochemistry-frozen sections: Figure 4E).
Flow-CS, IHC-Fr

Reviews for TRANCE/TNFSF11/RANK L Antibody (NB100-56748) (0)

There are no reviews for TRANCE/TNFSF11/RANK L Antibody (NB100-56748). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRANCE/TNFSF11/RANK L Antibody (NB100-56748). (Showing 1 - 1 of 1 FAQs).

  1. Has it been tested blocking/neutrazing capabilities block ligand-receptor interaction?
    • Our product NB100-56748 has unfortunately not been tested for blocking or neutralization yet, so we do not know if it will block the ligand/receptor interactions.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional TRANCE/TNFSF11/RANK L Products

Bioinformatics Tool for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)

Discover related pathways, diseases and genes to TRANCE/TNFSF11/RANK L Antibody (NB100-56748). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)

Discover more about diseases related to TRANCE/TNFSF11/RANK L Antibody (NB100-56748).
 

Pathways for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)

View related products by pathway.

PTMs for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)

Learn more about PTMs related to TRANCE/TNFSF11/RANK L Antibody (NB100-56748).
 

Research Areas for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)

Find related products by research area.

Blogs on TRANCE/TNFSF11/RANK L.

The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

Download our IHC Handbook

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TRANCE/TNFSF11/RANK L Antibody (12A380) - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFSF11
Entrez