TRAILR3/TNFRSF10C Antibody (JE58-51)

Western Blot: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Western blot analysis of TRAILR3/TNFRSF10C on different lysates with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: Raji cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27 kDa Observed band size: 27/70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32257) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Immunocytochemistry analysis of Jurkat cells labeling TRAILR3/TNFRSF10C with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Flow cytometric analysis of Jurkat cells labeling TRAILR3/TNFRSF10C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32257, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: JE58-51
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TRAILR3/TNFRSF10C Antibody (JE58-51) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human TRAILR3/TNFRSF10C aa 200-300. (Uniprot: O14798)
• Localization: Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TNFRSF10C
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TRAILR3/TNFRSF10C Antibody (JE58-51)

• Antagonist decoy receptor for TRAIL/Apo-2L
• CD263 antigen
• CD263
• cytotoxic TRAIL receptor-3
• DcR1
• DCR1MGC149502
• Decoy receptor 1
• Decoy TRAIL receptor without death domain
• LITMGC149501
• Lymphocyte inhibitor of TRAIL
• TNFRSF10C
• TRAIL R3
• TRAIL receptor 3
• TRAIL receptor without an intracellular domain
• TRAILR3
• TRAIL-R3
• TRAILR3TNF-related apoptosis-inducing ligand receptor 3
• TRIDDCR1-TNFR
• tumor necrosis factor receptor superfamily member 10C
• tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain

Background

DcR1 / TRAIL-R3 / TRID / LIT is one of the two putative decoy receptors identified for TRAIL, one of the members of TNF family of apoptosis inducing proteins. The other putative decoy receptor is called DcR2 / TRUNDD / TRAIL-R4. DcR1 is attached to the cell surface through glycophospholipid anchor. It has the extracellular TRAIL binding domain but lacks the cytoplasmic domain to induce apoptotic signal. Hence overexpression of DcR1 inhibits the TRAIL induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF10C