TRAIL/TNFSF10 Antibody [Biotin]

TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse TRAIL/TNFSF10 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1121) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Streptavidin-HRP (Catalog # DY998). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

TRAIL/TNFSF10 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse TRAIL/TNFSF10
  Pro118-Asn291
  Accession # P50592
• Specificity: Detects mouse TRAIL/TNFSF10 in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human TRAIL is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFSF10
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse TRAIL/TNFSF10 Antibody (Catalog # MAB1121)
  ELISA Detection: Mouse TRAIL/TNFSF10 Biotinylated Antibody (Catalog # BAF1121)
  Standard:Recombinant Mouse TRAIL/TNFSF10 (Catalog # 1121-TL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAIL/TNFSF10 Antibody [Biotin]

• Apo-2 ligand
• APO2L
• Apo-2Ltumor necrosis factor (ligand) family, member 10
• APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
• CD253 antigen
• CD253
• Protein TRAIL
• TL2
• TNF-related apoptosis-inducing ligand
• TNFSF10
• TRAIL
• TRAILTNF-related apoptosis inducing ligand TRAIL
• tumor necrosis factor (ligand) superfamily, member 10
• tumor necrosis factor ligand superfamily member 10

Background

TNF-related apoptosis-inducing ligand (TRAIL), also called apoptosis 2 ligand (Apo2L) for its similarity in sequence, structure, and function to Fas Ligand/Apo1L, is a 33‑35 kDa type II transmembrane glycoprotein of the tumor necrosis factor superfamily, designated TNFSF10 (1‑3). Mouse TRAIL cDNA encodes a 17 amino acid (aa) N-terminal intracellular domain, a 20 aa transmembrane domain and a 253 aa extracellular domain. Like most TNF family members, TRAIL is bioactive as a homotrimer (1). Unlike other TNF family members, a zinc ion complexed by human Cys 230 (mouse Cys 240) of each of the three monomers is critical for structural stability (4, 5). Either transmembrane or cysteine protease-released soluble sTRAIL induce apoptosis of many transformed cell lines, but rarely of normal cells (3, 6). Accordingly, TRAIL is suggested to have a role in tumor surveillance (1). Mice with genetically disrupted TRAIL have defective thymocyte apoptosis, creating faulty negative selection and some increased susceptibility to induced autoimmune diseases (7). In humans, TRAIL controls apoptosis of erythrocyte precursors and sTRAIL is inversely correlated with hemoglobin (1, 8). TRAIL transcripts are constitutively expressed in a variety of human (and presumably mouse) tissues and mononuclear cells (2, 3). Only one of two receptors that transduce apoptotic signals in humans is found in the mouse (TRAIL R2/DR5 but not TRAIL R1/DR4) (1). Mice express TRAIL receptors DcTRAIL R1/TNFRSF23 and DcTRAIL R2/TNFRSF22. These receptors lack death domains, but differ in structure from human regulatory receptors TRAIL R3 and TRAIL R4 (9). Osteoprotegerin has been identified in humans as a TRAIL receptor, but binding in mouse has not yet been demonstrated (1, 10). Mouse TRAIL shows 85% aa identity with rat TRAIL and 70% aa identity with human, bovine, and porcine TRAIL within the TNF homology domain (aa 118‑291).

1. Zauli, G. and P. Secchiero (2006) Cytokine Growth Factor Rev. 17:245.
2. Wiley, S.R. et al. (1995) Immunity 3:673.
3. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
4. Bodmer, J.L. et al. (2000) J. Biol. Chem. 275:20632.
5. Hymowitz, S.G. et al. (2000) Biochemistry 39:633.
6. Sedger, L. M. et al. (2002) Eur. J. Immunol. 32:2246.
7. Lamhamedi-Cherradi, S.E. et al. (2003) Nat. Immunol. 4:255.
8. Choi, J.W. (2005) Ann. Hematol. 84:728.
9. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
10. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAIL/TNFSF10 Antibody (BAF1121)(3)

Publications using BAF1121

• Starkey M, Nguyen D, Essilfie A, Kim R, Hatchwell L, Collison A, Yagita H, Foster P, Horvat J, Mattes J, Hansbro P Tumor necrosis factor-related apoptosis-inducing ligand translates neonatal respiratory infection into chronic lung disease. Mucosal Immunol, 2013-09-18;7(3):478-88. 2013-09-18 [PMID: 24045576] (ELISA Development (Detection), Mouse)
• Leu SW, Shi L, Xu C, Zhao Y, Liu B, Li Y, Shiedlin A, Xiang C, Shen H, Quinn DA, Hales CA, Zhao H TLR4 through IFN-beta promotes low molecular mass hyaluronan-induced neutrophil apoptosis. J. Immunol., 2010-11-22;186(1):556-62. 11/22/2010 [PMID: 21098223]
• Kim SM, Lim JY, Park SI, Jeong CH, Oh JH, Jeong M, Oh W, Park SH, Sung YC, Jeun SS Gene therapy using TRAIL-secreting human umbilical cord blood-derived mesenchymal stem cells against intracranial glioma. Cancer Res., 2008-12-01;68(23):9614-23. 2008-12-01 [PMID: 19047138] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: TNFSF10
• Uniprot:
  • P50592()