TRAIL/TNFSF10 Antibody (55B709.3) - BSA Free

Western Blot: TRAIL/TNFSF10 Antibody (55B709.3) [NB100-56518] - Analysis of TRAIL in Jurkat lysate

Immunohistochemistry-Paraffin: TRAIL/TNFSF10 Antibody (55B709.3) [NB100-56518] - Analysis of TRAIL in human kidney using TRAIL antibody at 5 ug/ml.

• Reactivity: Hu, Ca, Fe
• Applications: WB, IHC
• Clone: 55B709.3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TRAIL/TNFSF10 Antibody (55B709.3) - BSA Free Summary

• Description: Novus Biologicals Mouse TRAIL/TNFSF10 Antibody (55B709.3) - BSA Free (NB100-56518) is a monoclonal antibody validated for use in IHC and WB. Anti-TRAIL/TNFSF10 Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This monoclonal antibody was raised against a peptide corresponding to amino acids 17-35 of human TRAIL. This peptide sequence has been shown to be involved in TRAIL and DR5 (TRAIL-R2) interaction.
• Predicted Species: Canine (95%), Feline (95%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF10
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: In Jurkat, a 32 kDa band, which represents a membrane bound form of TRAIL, is observed.
• Control:

  Jurkat Whole Cell Lysate

Reactivity Notes

Predicted to react with Canine and Feline.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TRAIL/TNFSF10 Antibody (55B709.3) - BSA Free

• Apo-2 ligand
• APO2L
• Apo-2Ltumor necrosis factor (ligand) family, member 10
• APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
• CD253 antigen
• CD253
• Protein TRAIL
• TL2
• TNF-related apoptosis-inducing ligand
• TNFSF10
• TRAIL
• TRAILTNF-related apoptosis inducing ligand TRAIL
• tumor necrosis factor (ligand) superfamily, member 10
• tumor necrosis factor ligand superfamily member 10

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel member in the TNF family was recently identified and designated TRAIL (for TNF-related apoptosis-inducing ligand) and Apo-2L (for Apo-2 ligand) (6,7). TRAIL is a type II membrane protein and expressed in a variety of human tissues. Two novel death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL3-6. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAIL/TNFSF10 Antibody (NB100-56518)(9)

Publications using NB100-56518

• Jung BK, An YH, Jang SH et al. The tumor suppressive effect and apoptotic mechanism of TRAIL gene-containing recombinant NDV in TRAIL-resistant colorectal cancer HT-29 cells and TRAIL-nonresistant HCT116 cells, with each cell bearing a mouse model Cancer medicine 2023-10-16 [PMID: 37843231] (WB)
  
  Details:
  Dilution 1:1000
• Sullivan Gp, O'Connor H, Henry Cm Et Al. TRAIL Receptors Serve as Stress-Associated Molecular Patterns to Promote ER-Stress-Induced Inflammation Dev. Cell 2020-03-23 [PMID: 32109381] (KD, WB, Human)
• Iurlaro R, Puschel F, Leon-Annicchiarico CL et al. Glucose deprivation induces ATF4-mediated apoptosis through TRAIL death receptors. Mol. Cell. Biol. 2017-02-27 [PMID: 28242652] (WB, Human)
• Toriyama S, Horinaka M, Yasuda S et al. A histone deacetylase inhibitor OBP-801 and celecoxib synergistically inhibit the cell growth with apoptosis via a DR5-dependent pathway in bladder cancer cells Mol. Cancer Ther. 2016-07-12 [PMID: 27406983] (WB)
• Lamas B, Goncalves-Mendes N, Nachat-Kappes R et al. Leptin modulates dose-dependently the metabolic and cytolytic activities of NK-92 cells. J Cell Physiol. 2013-06-01 [PMID: 23129404]
• Leonardi R, Almeida LE, Rusu M et al. Tumor necrosis factor-related apoptosis-inducing ligand expression correlates to temporomandibular joint disk degeneration. J Craniofac Surg. 2011-03-01 [PMID: 21403533]
• Huang SC, Tsai HF, Tzeng HT et al. Lipid raft assembly and Lck recruitment in TRAIL costimulation mediates NF-kB activation and T cell proliferation. J Immunol. 2011-01-15 [PMID: 21160038] (WB)
  
  Details:
  WB: Fig 6 (Jurkat).Note: The specificity of the antibody was validated with TRAIL siRNA in Jurkat by WB in Fig 6. TRAIL siRNA knocked down endogeneous TRAIL expression in Jurkat by approximately two thirds.
• Jin F, Liu X, Zhou Z et al. Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells. Cancer Res. 2005-07-15 [PMID: 16024638] (WB)
  
  Details:
  IMGENEX antibodies cited for WB: 1. Caspase-3 mAb, clone 31A1067 (IMG-144A): Fig 3C, DU145 prostate adenocarcinoma cells. 2. DcR1 pAb (IMG-245-1/IMG-245-2): Fig 6C, DcR1 overexpressing DU145 cells. Note: The specificity of the DcR1 pAb was validated in Dc
• Muller DB, Raftery MJ, Kather A et al. Frontline: Induction of apoptosis and modulation of c-FLIPL and p53 in immature dendritic cells infected with herpes simplex virus. Eur J Immunol. 2004-04-01 [PMID: 15048704]

Bioinformatics

• Gene Symbol: TNFSF10
• Entrez:
  • 8743(Human)
• Uniprot:
  • P50592()
  • NP_003801.1(Human)