TRAIL/TNFSF10 Antibody (55B709.3)

Images

 
Western Blot: TRAIL/TNFSF10 Antibody (55B709.3) [NB100-56518] - Analysis of TRAIL in Jurkat lysate
Immunohistochemistry-Paraffin: TRAIL/TNFSF10 Antibody (55B709.3) [NB100-56518] - Analysis of TRAIL in human kidney using TRAIL antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Ca, FeSpecies Glossary
Applications WB, IHC-P
Clone
55B709.3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

TRAIL/TNFSF10 Antibody (55B709.3) Summary

Immunogen
This monoclonal antibody was raised against a peptide corresponding to amino acids 17-35 of human TRAIL. This peptide sequence has been shown to be involved in TRAIL and DR5 (TRAIL-R2) interaction.
Specificity
In Jurkat, a 32 kDa band, which represents a membrane bound form of TRAIL, is observed.
Predicted Species
Feline (95%), Canine (95%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFSF10
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Positive Control
Jurkat Lysate (NB800-PC2)
Publications
Read Publications using
NB100-56518 in the following applications:

  • WB
    4 publications

Reactivity Notes

Cross reacts with Human. Predicted to react with Canine and Feline.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for TRAIL/TNFSF10 Antibody (55B709.3)

  • Apo-2 ligand
  • APO2L
  • Apo-2Ltumor necrosis factor (ligand) family, member 10
  • APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
  • CD253 antigen
  • CD253
  • Protein TRAIL
  • TL2
  • TNF-related apoptosis-inducing ligand
  • TNFSF10
  • TRAIL
  • TRAILTNF-related apoptosis inducing ligand TRAIL
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor ligand superfamily member 10

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel member in the TNF family was recently identified and designated TRAIL (for TNF-related apoptosis-inducing ligand) and Apo-2L (for Apo-2 ligand) (6,7). TRAIL is a type II membrane protein and expressed in a variety of human tissues. Two novel death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL3-6. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRAIL/TNFSF10 Antibody (NB100-56518)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
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Filter By Species
Human
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-56518 Applications Species
Iurlaro R, Puschel F, Leon-Annicchiarico CL et al. Glucose deprivation induces ATF4-mediated apoptosis through TRAIL death receptors. Mol. Cell. Biol. Feb 27 2017 [PMID: 28242652] (WB, Human) WB Human
Toriyama S, Horinaka M, Yasuda S et al. A histone deacetylase inhibitor OBP-801 and celecoxib synergistically inhibit the cell growth with apoptosis via a DR5-dependent pathway in bladder cancer cells Mol. Cancer Ther. 2016 Jul 12 [PMID: 27406983] (WB) WB
Lamas B, Goncalves-Mendes N, Nachat-Kappes R et al. Leptin modulates dose-dependently the metabolic and cytolytic activities of NK-92 cells. J Cell Physiol. 2013 Jun [PMID: 23129404]
Leonardi R, Almeida LE, Rusu M et al. Tumor necrosis factor-related apoptosis-inducing ligand expression correlates to temporomandibular joint disk degeneration. J Craniofac Surg. 2011 Mar [PMID: 21403533]
Huang SC, Tsai HF, Tzeng HT et al. Lipid raft assembly and Lck recruitment in TRAIL costimulation mediates NF-kB activation and T cell proliferation. J Immunol. 2011 Jan 15 [PMID: 21160038] (WB)

Details:
WB: Fig 6 (Jurkat).Note: The specificity of the antibody was validated with TRAIL siRNA in Jurkat by WB in Fig 6. TRAIL siRNA knocked down endogeneous TRAIL expression in Jurkat by approximately two thirds.
WB
Jin F, Liu X, Zhou Z et al. Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells. Cancer Res. 2005 Jul 15 [PMID: 16024638] (WB)

Details:
WB: 1. Caspase-3 mAb, clone 31A1067 (IMG-144A): Fig 3C, DU145 prostate adenocarcinoma cells.
WB
Muller DB, Raftery MJ, Kather A et al. Frontline: Induction of apoptosis and modulation of c-FLIPL and p53 in immature dendritic cells infected with herpes simplex virus. Eur J Immunol. 2004 Apr [PMID: 15048704]

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TRAIL/TNFSF10 Antibody (NB100-56518) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56518AF405
Alexa Fluor 488 Labeled NB100-56518AF488
Alexa Fluor 647 Labeled NB100-56518AF647
Alexa Fluor 700 Labeled NB100-56518AF700
Biotin Labeled NB100-56518B
DyLight 350 Labeled NB100-56518UV
DyLight 405 Labeled NB100-56518V
DyLight 488 Labeled NB100-56518G
DyLight 550 Labeled NB100-56518R
DyLight 650 Labeled NB100-56518C
DyLight 680 Labeled NB100-56518FR
DyLight 755 Labeled NB100-56518IR
HRP Labeled NB100-56518H

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Bioinformatics

Gene Symbol TNFSF10
Entrez
Uniprot