TRAF-6 Antibody - BSA Free

Western Blot: TRAF-6 Antibody [NBP1-77230] - PC-3 cell lysates with TRAF-6 antibody at 1 ug/ml in the presence (A) or absence (B) of 1 ug blocking peptide.

Immunocytochemistry/ Immunofluorescence: TRAF-6 Antibody - BSA Free [NBP1-77230] - Immunocytochemistry of TRAF-6 in K562 cells with TRAF-6 antibody at 0.5 u/mL.

Immunocytochemistry/ Immunofluorescence: TRAF-6 Antibody - BSA Free [NBP1-77230] - Immunofluorescence of TRAF-6 in K562 cells with TRAF-6 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRAF-6 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human TRAF6. The immunogen is located within the last 50 amino acids of TRAF6. Amino Acid Squence: RREGFQPRSTDAGV
• Predicted Species: Porcine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAF6
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/mL
  • Western Blot 1 ug/ml
• Control Peptide:

  TRAF-6 Peptide (NBP1-77230PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for TRAF-6 Antibody - BSA Free

• E3 ubiquitin-protein ligase TRAF6
• EC 6.3.2.-
• Interleukin-1 signal transducer
• RING finger protein 85
• RNF85MGC:3310
• TNF receptor-associated factor 6
• TRAF6
• TRAF-6

Background

Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-kappaB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-6 Antibody (NBP1-77230)(1)

Publication using NBP1-77230

• Doyon P, Servant MJ. Tumor necrosis factor receptor-associated factor-6 and ribosomal S6 kinase intracellular pathways link the angiotensin II AT1 receptor to the phosphorylation and activation of the IkappaB kinase complex in vascular smooth muscle cells. J Biol Chem. 2010-10-01 [PMID: 20659889]
  
  Details:
  TRAF6 (IMG-5654-2). WB: Low passage primary rat aortic VSMCs siRNA or non-specific TRAF6 transfected cell line, Fig 3B. Note: TRAF6 (IMG-5654-2) was siRNA validated by WB in siRNA TRAF6 transfected VSMC cell line, Fig 3B.

Bioinformatics

• Gene Symbol: TRAF6
• Uniprot:
  • NP_004611(Human)